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The map label for this gene is tgt [H]

Identifier: 195952598

GI number: 195952598

Start: 198514

End: 199644

Strand: Direct

Name: tgt [H]

Synonym: HY04AAS1_0218

Alternate gene names: 195952598

Gene position: 198514-199644 (Clockwise)

Preceding gene: 195952597

Following gene: 195952599

Centisome position: 12.73

GC content: 34.84

Gene sequence:

>1131_bases
ATGTTTAAGATAGAAAAAACCAATGGTTTAGCAAGGGCTGGTATATTATCAACTTTACATGGTGATATATTAACACCTGT
TTTTATGCCGGTGGGTACAAAGGGTACTGTAAAAGCCATGACTCACCGTTTGTTAGAAGAGCTTGGTACTCAAATCATCC
TTGGAAATACCTATCATCTTTACTTAAGGCCAGGACCTGAGCTTATAGAAAAGTTTGGTGGTCTTCATAAATTTATCAAC
TGGAATAAGCCTATTCTTACAGATAGCGGTGGTTTTCAAGTATTTTCTTTGTCTAAGAAAAATAGCTCAAACGTCAAGAT
AACAGATGATGGAGTTTATTTTAAAGACCACCTTCAAGGGGATAAACATCTTTTTAGCCCTGAGAAAGTGATAGAAATTC
AAGAGATATTTGGCTCAGATATCATGATGCCAATAGATGAGTGTATCTCTTTACCGGCTTCTTACGAATATACCAAAGAA
GCTCTTTATAGGACTATAAGATGGCTAGAGCGTTCCAAAAAAGCCAAAAAACGAAAAGACCAAATGCTTTTTGGCATTGT
TCAAGGCGGTGAATATAAAGATTTAAGAAAGCTTTCTGCAAGACTTACTGTAGAATTTGATATGGATGGTTATTCTATCG
GTGGCTTGTCTGTGGGGGAGTCAAAAGATATCATGTATTCTATGACCTATTACGTTGTAGAAGAACTGCCGTTTGATAAA
CCCCATTATCTTATGGGCGTTGGAAAACCAGAGGATATATTGGAAGCCATTTCTCTTGGAATAGATATGTTTGATTGTGT
GATTCCTACAAGAAACGCCCGTACTGGGCTTTTATACACATCAAAAGGTGAACTTGTTTTAAGACATGAGCGTTATAAAG
AAGATCCAAGACCTGTGGATGAAGAATGTGAATGTTATACCTGTAAAAACTTTTCAAGGGCTTATTTGAGACATCTTTTT
ATGATGGAGGAAATATCTTCTTATATTTTAAATACTATTCACAATCTTCACTTTTTCTTAAATCTTACCAAAAAAGCCAG
AGAAGCTATAATCCAAGGTAGGTTTTTGGATTTTAAAAGCCAAATGTTAGAAAAGTTTAATATGAAAAATCATAGCATAA
ACTTAAACTAA

Upstream 100 bases:

>100_bases
AAGGAAAAACTTATTAAAAGCTCATGGCTCAAGCGTTATAGGAAATTTGTAAAAGATGCTTCAGAGGGTGCTATTTTATC
TGCGGATTGACATTTAGTTG

Downstream 100 bases:

>100_bases
ATCTAAATTTTAAACTATGAATTTAAAATCTTGGAGTTATTATATACAGCTAAGAGCTTACGATGAATCTGGAAATGTAA
AAGAGGATTCTGCCATAGTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFKIEKTNGLARAGILSTLHGDILTPVFMPVGTKGTVKAMTHRLLEELGTQIILGNTYHLYLRPGPELIEKFGGLHKFIN
WNKPILTDSGGFQVFSLSKKNSSNVKITDDGVYFKDHLQGDKHLFSPEKVIEIQEIFGSDIMMPIDECISLPASYEYTKE
ALYRTIRWLERSKKAKKRKDQMLFGIVQGGEYKDLRKLSARLTVEFDMDGYSIGGLSVGESKDIMYSMTYYVVEELPFDK
PHYLMGVGKPEDILEAISLGIDMFDCVIPTRNARTGLLYTSKGELVLRHERYKEDPRPVDEECECYTCKNFSRAYLRHLF
MMEEISSYILNTIHNLHFFLNLTKKAREAIIQGRFLDFKSQMLEKFNMKNHSINLN

Sequences:

>Translated_376_residues
MFKIEKTNGLARAGILSTLHGDILTPVFMPVGTKGTVKAMTHRLLEELGTQIILGNTYHLYLRPGPELIEKFGGLHKFIN
WNKPILTDSGGFQVFSLSKKNSSNVKITDDGVYFKDHLQGDKHLFSPEKVIEIQEIFGSDIMMPIDECISLPASYEYTKE
ALYRTIRWLERSKKAKKRKDQMLFGIVQGGEYKDLRKLSARLTVEFDMDGYSIGGLSVGESKDIMYSMTYYVVEELPFDK
PHYLMGVGKPEDILEAISLGIDMFDCVIPTRNARTGLLYTSKGELVLRHERYKEDPRPVDEECECYTCKNFSRAYLRHLF
MMEEISSYILNTIHNLHFFLNLTKKAREAIIQGRFLDFKSQMLEKFNMKNHSINLN
>Mature_376_residues
MFKIEKTNGLARAGILSTLHGDILTPVFMPVGTKGTVKAMTHRLLEELGTQIILGNTYHLYLRPGPELIEKFGGLHKFIN
WNKPILTDSGGFQVFSLSKKNSSNVKITDDGVYFKDHLQGDKHLFSPEKVIEIQEIFGSDIMMPIDECISLPASYEYTKE
ALYRTIRWLERSKKAKKRKDQMLFGIVQGGEYKDLRKLSARLTVEFDMDGYSIGGLSVGESKDIMYSMTYYVVEELPFDK
PHYLMGVGKPEDILEAISLGIDMFDCVIPTRNARTGLLYTSKGELVLRHERYKEDPRPVDEECECYTCKNFSRAYLRHLF
MMEEISSYILNTIHNLHFFLNLTKKAREAIIQGRFLDFKSQMLEKFNMKNHSINLN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=46.1971830985916, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1786607, Length=380, Percent_Identity=47.3684210526316, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=356, Percent_Identity=44.1011235955056, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=168, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=44.6629213483146, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21355311, Length=250, Percent_Identity=26, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43276; Mature: 43276

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIEKTNGLARAGILSTLHGDILTPVFMPVGTKGTVKAMTHRLLEELGTQIILGNTYHL
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEE
YLRPGPELIEKFGGLHKFINWNKPILTDSGGFQVFSLSKKNSSNVKITDDGVYFKDHLQG
EECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCEEEECCCEEEHHHCCC
DKHLFSPEKVIEIQEIFGSDIMMPIDECISLPASYEYTKEALYRTIRWLERSKKAKKRKD
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QMLFGIVQGGEYKDLRKLSARLTVEFDMDGYSIGGLSVGESKDIMYSMTYYVVEELPFDK
HEEEEEECCCCHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
PHYLMGVGKPEDILEAISLGIDMFDCVIPTRNARTGLLYTSKGELVLRHERYKEDPRPVD
CCEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCEEEEEHHCCCCCCCCC
EECECYTCKNFSRAYLRHLFMMEEISSYILNTIHNLHFFLNLTKKAREAIIQGRFLDFKS
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCHHHHHHH
QMLEKFNMKNHSINLN
HHHHHHCCCCCEEECC
>Mature Secondary Structure
MFKIEKTNGLARAGILSTLHGDILTPVFMPVGTKGTVKAMTHRLLEELGTQIILGNTYHL
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEE
YLRPGPELIEKFGGLHKFINWNKPILTDSGGFQVFSLSKKNSSNVKITDDGVYFKDHLQG
EECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCEEEECCCEEEHHHCCC
DKHLFSPEKVIEIQEIFGSDIMMPIDECISLPASYEYTKEALYRTIRWLERSKKAKKRKD
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QMLFGIVQGGEYKDLRKLSARLTVEFDMDGYSIGGLSVGESKDIMYSMTYYVVEELPFDK
HEEEEEECCCCHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
PHYLMGVGKPEDILEAISLGIDMFDCVIPTRNARTGLLYTSKGELVLRHERYKEDPRPVD
CCEEEECCCHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCEEEEEHHCCCCCCCCC
EECECYTCKNFSRAYLRHLFMMEEISSYILNTIHNLHFFLNLTKKAREAIIQGRFLDFKS
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCHHHHHHH
QMLEKFNMKNHSINLN
HHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]