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The map label for this gene is poxB [H]

Identifier: 194737868

GI number: 194737868

Start: 1020509

End: 1022227

Strand: Reverse

Name: poxB [H]

Synonym: SeSA_A1052

Alternate gene names: 194737868

Gene position: 1022227-1020509 (Counterclockwise)

Preceding gene: 194738339

Following gene: 194737433

Centisome position: 21.71

GC content: 57.01

Gene sequence:

>1719_bases
ATGAAACAAACCGTTGCTGCTTTTATCGCCAAAACGCTGGAACAGGCTGGCGTAAAACGAATCTGGGGCGTCACTGGCGA
CTCCCTGAACGGCCTGAGCGATAGTCTTAATCGTATGGGAACCATTGAATGGATGCCAACCCGCCACGAAGAGGTCGCCG
CTTTCGCCGCCGGCGCACAAGCGCAGCTTACCGGCGAGCTGGCAGTGTGCGCCGGTTCATGTGGACCGGGCAACCTGCAC
CTGATCAATGGCCTGTTTGATTGCCACCGTAACCACGTCCCTGTTCTGGCGATCGCCGCCCATATTCCGTCCAGTGAAAT
CGGTAGCGGTTATTTCCAGGAGACCCACCCGCAGGAGTTGTTCCGTGAATGCAGCCACTATTGCGAGTTGGTTTCCAGCC
CGGAGCAGATCCCGCAGGTACTCGCCATCGCCATGCGCAAAGCGGTGCTAAATCGCGGCGTCTCCGTGGTCGTACTTCCG
GGTGACGTAGCGCTGAAACCCGCGCCTGAAAACGCCGTCACTCATTGGTACCATGCCCCGCACCCCGTCGTCACGCCGGC
GGAGGAAGAGCTGAAAAAACTGGCGCAACTGCTGCGCTACTCCAGCAATATCGCATTGATGTGCGGTAGCGGCTGCGCGG
GCGCGCATGAGGAGTTGGTCGCGTTGGCGGCAAAACTAAAAGCGCCAATCGTTCATGCCCTGCGCGGCAAAGAGCACGTT
GAGTACGATAACCCTTATGATGTGGGAATGACCGGCCTGATTGGCTTTTCTTCTGGCTTCCACACCATGATGAACGCCGA
TACGCTGATCCTGCTCGGCACCCAGTTCCCCTACCGCGCCTTTTATCCGAGCGATGCCAAAATCATTCAGATTGACATCA
ACCCGGGTAGTATTGGCGCACACAGCAAAGTCGACATGGCGCTGGTGGGCGATATTAAAGCCACGCTTCGCGCCCTGCTG
CCGCTGGTGGAAGAAAAAAGCAATCGTAAATTCCTCGATAAAGCTCTGGAGCACTATCGGGACGCCCGTAAGGGACTTGA
TGACTTAGCCAAACTCAGCGACAAAGCCATCCATCCGCAATATCTGGCGCAACAAATTAGTCATTTCGCCGCTGACGACG
CTATTTTTACCTGCGATGTCGGCACGCCGACCGTCTGGGCGGCCCGCTATCTAAAAATGAACGGCAAGCGCCGCTTGCTG
GGCTCGTTTAACCACGGGTCAATGGCCAACGCGATGCCGCAGGCGCTCGGCGCTCAGGCCACCGCGCCTGGTCGTCAGGT
TATCGCGATGTGCGGTGATGGCGGCTTCAGTATGCTGATGGGCGATTTTCTCTCGGTGGTACAGATGAAGCTGCCGATAA
AAATCGTCGTCTTTAATAACAGCGTGCTGGGCTTTGTGGCGATGGAAATGAAAGCCGGCGGCTACCTTACCGACGGTACC
GAACTGCACGACACCAATTTCGCGCGGATCGCCGAAGCCTGCGGCATTACCGGTATTTGCGTGGAAAAAGCCGCAGACGT
GGACGGAGCGCTACAGCGCGCGTTTTCCATTGACGGCCCGGTACTGGTTGACGTGGTCGTGGCAAAAGAAGAGCTGGCGA
TCCCGCCGCAGATCAAACTGGAACAAGCGAAAGGCTTTAGCCTGTACATGCTACGCGCCATTATTAGCGGCCGCGGCGAT
GAAGTGATCGAACTGGCGAAAACGAACTGGCTAAGGTAA

Upstream 100 bases:

>100_bases
CTCACTTCATCCTTTCGCCCCTCTCTTCGCCAAAGAAAAACGTCATCGTCATCTTCAACTAAACTTGTAACCGTTATCAC
ATTTAAGGAGATGGAGAACC

Downstream 100 bases:

>100_bases
AACAGTGCACATCACCTTTTAAAATCCAGGGATGTGCCATGATTGATTTACGCAGTGATACCGTTACCCGACCGGGCCGC
GCCATGCTCGAGGCGATGAT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKQTVAAFIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGICVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Sequences:

>Translated_572_residues
MKQTVAAFIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGICVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR
>Mature_572_residues
MKQTVAAFIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGICVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=24.5080500894454, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=94.2307692307692, Blast_Score=1098, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=29.6428571428571, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=28.3121597096189, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=24.8175182481752, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788716, Length=543, Percent_Identity=22.8360957642726, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=26.4653641207815, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=26.4653641207815, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=25.0447227191413, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.6102941176471, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=551, Percent_Identity=25.5898366606171, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61683; Mature: 61683

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVAAFIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQ
CCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPENAVTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCEEECCC
HPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HSKVDMALVGDIKATLRALLPLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQ
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGICVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVAAFIAKTLEQAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQ
CCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPENAVTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCEEECCC
HPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HSKVDMALVGDIKATLRALLPLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQ
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGICVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]