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The map label for this gene is nfdA [H]

Identifier: 194737712

GI number: 194737712

Start: 1515270

End: 1516970

Strand: Reverse

Name: nfdA [H]

Synonym: SeSA_A1571

Alternate gene names: 194737712

Gene position: 1516970-1515270 (Counterclockwise)

Preceding gene: 194737249

Following gene: 194738016

Centisome position: 32.21

GC content: 56.97

Gene sequence:

>1701_bases
ATGATCAGTCTCGTCCCGTCTCTGATTTCCAGAACCGCGCTTCTCTTTCTGCTCACGGCCACAGGCGCAGCCACCGCCGC
CCGCCCGGCAGCGGATATTATTTTGCATAACGGTAATATCATCACTCTGAATGATGCCCAGCCGCAGGCCAGCGCGCTGG
CGATTTCCGGCTCGCGGATTGTGGCGATTGGCGGTGATACGGCGACAAATGAATGGCGCGGCGACCATACCCGTACCATC
GATTTACAGGGTAAAACCGTGATACCCGGCCTGACCGATACCCACATTCACGCCATTCGCGGCGGACAAACCTGGACATT
CGAAACCTACTGGTACAACAGCCCTTCGCTTAAAGACGCGCTGGATAAATTACGCGCCGACGCTAACCGTCGTCCCCGCG
ATCAATGGGTAGCCGTAGTGGGATCGTGGATACCGGCACAATTTGCAGAAAACCGGGCGCCGACGGTAGCCGAACTGAGC
CACGCCCTTCCCGATCATCCGGCTTATATTCAGTATCTTTACGACTATGCGTTAGTGAATCAGCGCGGTATAGACGTACT
TGGCCTTAACGACACCCCTCCTCCTGATTTAGCGGGAATCCGCGTAGAGCGCGACACAAAAGGTAGCGCCACGGGGAAAT
TATTTGGTGACATCGCCGCGTTTAACCAGCTTTTTGCCAGCATAAGTAGTAACGCCGATCGCGAGGGCGGTCTGCGACAA
TTTTTCGCTGATATGAACGCTCGCGGCGTGACCGGCATCATTGACCCCTCTGCCGGGCCTGCCGCCGCTTATGAGCCTTT
ATTTGCAATGCGAAACCAGGGGGATTTACCGCTGCGCGTGGGGTATCGCATTCCGGTACAGCCGGAAGCGAAAGGTCATG
AAGCGCAGTGGTTCAGCAACCTGATGGCCTTTCGCCCGGCGCGTGCCGATGACGGACAACTGGCTTTTCTTGGCCTGGGG
GAAAGCCTGGTGGCCGGAATGAATGACGGCGTGCGGATGGCCCCAGGATTTTCTTCCTCAGAGCAGGATAAAACCGCGCT
TCGCCAGGTCGCGACGTTTGCGGCAAAACGGGGAATACCGCTTGAGATCCACGCCTATACCGATGACAGCGCCGACGCGA
TATTGACGATTTTTGAGCAGGTAGCGCAGCAGTACGATCTGCGTCCTCTCCGCTGGTCTATTGCGCATCTGAATACCGGT
TCGCCACAGACGCTTGAGCGAATGCGTAAGCTGGGCCTGGCATACACTGTGCAAATGGGGCCTTACTTTGAGGGGCTTGC
CATCCGTGACGCCAATCCACCCGGCGCGACGGACAATTCGCCGCCGGTTCGACTGGCGCTGGATAAAGGGCTTGTCGTAG
CTGGCGGTACCGATTCGACGCGTATTGGCATTGCCGGTGTCTGGCACGCTATCGAATATCATATCACCGGTATAGCGTCA
GGCGGTTCCGTACGTAAACCCGCCAGCGAGCGGCTCACGCGTCTGGAAGCGCTAGCGTTATATACACGTCATGCCGCCTG
GCTCGCCTTTGCCGAACAACACCGGGGCCAGCTTAGCGTCGGAAAACAGGCCGATCTGGCGGTACTCAATCAGCCATTTA
TGACGATGCCCGAAGACAGAATTGATACCATTCGCGCTGTTTTGACGCTTGTCGATGGACGCATTGTTCACGAAAGTCCG
GACCTTAACGCCGGACAATGA

Upstream 100 bases:

>100_bases
ACCGTTCTAAAAACAGAACGCACTGTTCCCTGGGCACAGATTGAACAATTCTTGTTCGCTCTCTATGGTTTGCTTATCGC
CCGACAAAAGGAACATGCTA

Downstream 100 bases:

>100_bases
GGTTGTCAGTTGACGCAAAGCCTGCTTTTATAACCCACCAGCGGCGCTGACGGGAAATCTTTTCCGCCAGCGCCGCGATA
GAGTTAATGTATTGAGATCC

Product: putative amidohydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MISLVPSLISRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGGDTATNEWRGDHTRTI
DLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYNSPSLKDALDKLRADANRRPRDQWVAVVGSWIPAQFAENRAPTVAELS
HALPDHPAYIQYLYDYALVNQRGIDVLGLNDTPPPDLAGIRVERDTKGSATGKLFGDIAAFNQLFASISSNADREGGLRQ
FFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLGLG
ESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTG
SPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGIAS
GGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLSVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHESP
DLNAGQ

Sequences:

>Translated_566_residues
MISLVPSLISRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGGDTATNEWRGDHTRTI
DLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYNSPSLKDALDKLRADANRRPRDQWVAVVGSWIPAQFAENRAPTVAELS
HALPDHPAYIQYLYDYALVNQRGIDVLGLNDTPPPDLAGIRVERDTKGSATGKLFGDIAAFNQLFASISSNADREGGLRQ
FFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLGLG
ESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTG
SPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGIAS
GGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLSVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHESP
DLNAGQ
>Mature_566_residues
MISLVPSLISRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGGDTATNEWRGDHTRTI
DLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYNSPSLKDALDKLRADANRRPRDQWVAVVGSWIPAQFAENRAPTVAELS
HALPDHPAYIQYLYDYALVNQRGIDVLGLNDTPPPDLAGIRVERDTKGSATGKLFGDIAAFNQLFASISSNADREGGLRQ
FFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLGLG
ESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTG
SPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGIAS
GGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLSVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHESP
DLNAGQ

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 61185; Mature: 61185

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLVPSLISRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCEE
VAIGGDTATNEWRGDHTRTIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYNSPSLKDA
EEECCCCCCCCCCCCCEEEEEECCCEECCCCCCCEEEEEECCCEEEEEEEEECCCCHHHH
LDKLRADANRRPRDQWVAVVGSWIPAQFAENRAPTVAELSHALPDHPAYIQYLYDYALVN
HHHHHHCCCCCCHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
QRGIDVLGLNDTPPPDLAGIRVERDTKGSATGKLFGDIAAFNQLFASISSNADREGGLRQ
CCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
FFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSN
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHHHH
LMAFRPARADDGQLAFLGLGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIP
HHHHCCCCCCCCCEEEEECCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
LEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTGSPQTLERMRKLGLAYTVQMG
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
PYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGIAS
CCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHEEEEEEEEEEC
GGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLSVGKQADLAVLNQPFMTMPEDR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHH
IDTIRAVLTLVDGRIVHESPDLNAGQ
HHHHHHHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure
MISLVPSLISRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCEE
VAIGGDTATNEWRGDHTRTIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYNSPSLKDA
EEECCCCCCCCCCCCCEEEEEECCCEECCCCCCCEEEEEECCCEEEEEEEEECCCCHHHH
LDKLRADANRRPRDQWVAVVGSWIPAQFAENRAPTVAELSHALPDHPAYIQYLYDYALVN
HHHHHHCCCCCCHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
QRGIDVLGLNDTPPPDLAGIRVERDTKGSATGKLFGDIAAFNQLFASISSNADREGGLRQ
CCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
FFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSN
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHHHH
LMAFRPARADDGQLAFLGLGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIP
HHHHCCCCCCCCCEEEEECCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
LEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTGSPQTLERMRKLGLAYTVQMG
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
PYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGIAS
CCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHEEEEEEEEEEC
GGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLSVGKQADLAVLNQPFMTMPEDR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHH
IDTIRAVLTLVDGRIVHESPDLNAGQ
HHHHHHHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA