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The map label for this gene is rstB [H]

Identifier: 194738016

GI number: 194738016

Start: 1513857

End: 1515158

Strand: Reverse

Name: rstB [H]

Synonym: SeSA_A1570

Alternate gene names: 194738016

Gene position: 1515158-1513857 (Counterclockwise)

Preceding gene: 194737712

Following gene: 194734192

Centisome position: 32.18

GC content: 54.07

Gene sequence:

>1302_bases
ATGAAAAAGCTGTTTGTGCAGTTTTACCTTTTGCTGTTCGTCTGTTTTCTGGTCATGACCTTATTGGTCGGACTGGTGTA
CAAATTCACTGCCGAACGCGCGGGCAGGCAATCGCTCGACGATCTGATGAAAAGTTCGCTCTATCTGATGCGCAGCGAAC
TGCGGGAAATTCCGCCGCGTGAATGGGGGAAAACGTTAAAAGAGATGGACCTGAACCTCTCTTTCGATTTACGCGTTGAA
CCGTTAAATCACTATAAGCTTGATGCCGCCACCACGCAGCGACTGCGTGAAGGCGATATTGTCGCGCTTGACGATCAATA
CACCTTTATTCAGCGTATCCCGCGCAGCCATTACGTCCTGGCGGTTGGCCCGGTTCCCTACCTCTATTTCCTGCATCAAA
TGCGACTGCTGGATATCGCCTTAATGGCGCTCATTGCCTTTTCTCTCGCCTTTCCTGTGTTTATCTGGATGCGCCCGCAC
TGGCAAGAAATGCTCAGGCTCGAATCCGCCGCGCAGCGCTTTGGCGAGGGTCATTTGACTGAACGCCTGCATTTCGATAA
TGGTTCCAGTTTCGAACGTCTGGGCGTCGCGTTCAACCAGATGGCGGATAATATTAATGCTCTGATCGCCAGTAAAAAAC
AGCTGATTGACGGTATCGCCCATGAGCTTCGCACGCCGCTGGTACGTTTACGCTATCGGCTTGAAATGAGTGAAAACCTT
ACACCGCCGGAATCACAGGCGCTCAATCGCGATATTGGTCAACTGGAAGCGCTGATTGAAGAACTGCTCACCTATGCGCG
CCTTGACCGACCACAAAATGAACTGATGTTGACCGAACCCGACCTCCCCGCCTGGTTGCTCGCGCATTTGCAGGATGTGC
AGAGCGTGACCCCGGAACGCGCCGTAAACCTTCTCACATCTGTAATCGGCGATTATGGCGCGCTTGATATGCGGTTAATG
TCGCGAGTGCTGGATAACCTGCTGAATAATGCGTTGCGCTACAGTCGTACCACCGTTCAGGTCAGTCTGTTACTCGACGG
CAGTCAGGCAGCGTTGATAGTCGAAGATGACGGCCCCGGCATTGAAGCGGATGCCCGAGAGCGCGTATTTGAACCTTTTG
TCAGACTCGACCCCAGTAGAGACCGGGCGACAGGCGGCTGCGGGCTTGGACTCGCCATCGTACACTCTATCGCCCTGGCG
ATGGGCGGTAGCGTCGTCTGTGACGAGAGCGAACTGGGCGGCGCAAAATTTAGCTTTCACTGGCCGGTCTGGCACGCGAT
GCCCGACATGACCGCTGCCTGA

Upstream 100 bases:

>100_bases
GACGCAAAGCCTGCTTTTATAACCCACCAGCGGCGCTGACGGGAAATCTTTTCCGCCAGCGCCGCGATAGAGTTAATGTA
TTGAGATCCGGTGGGCGTTG

Downstream 100 bases:

>100_bases
CGTATTTTGCACGTTGGTTGAGCGGCGGGAACTGTGCTATACAGATAGTATGTTGTAACTAAAGTGAGTAATACCATGTC
CCGCTACGATCTCGTTGAAC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLLTSVIGDYGALDMRLM
SRVLDNLLNNALRYSRTTVQVSLLLDGSQAALIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFHWPVWHAMPDMTAA

Sequences:

>Translated_433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLLTSVIGDYGALDMRLM
SRVLDNLLNNALRYSRTTVQVSLLLDGSQAALIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFHWPVWHAMPDMTAA
>Mature_433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLLTSVIGDYGALDMRLM
SRVLDNLLNNALRYSRTTVQVSLLLDGSQAALIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFHWPVWHAMPDMTAA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=81.7551963048499, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=31.8840579710145, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=30.6569343065693, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=29.8892988929889, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786783, Length=248, Percent_Identity=32.258064516129, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=28.4444444444444, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789403, Length=241, Percent_Identity=29.045643153527, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=26.953125, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082128, Length=259, Percent_Identity=30.1158301158301, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=27.9475982532751, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=232, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788279, Length=291, Percent_Identity=27.1477663230241, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787374, Length=243, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=26.2222222222222, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=27.7533039647577, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.8181818181818, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49144; Mature: 49144

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
ERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPER
CCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHH
AVNLLTSVIGDYGALDMRLMSRVLDNLLNNALRYSRTTVQVSLLLDGSQAALIVEDDGPG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDESELGGAKFSFH
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
WPVWHAMPDMTAA
CCHHHCCCCCCCC
>Mature Secondary Structure
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
ERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPER
CCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHH
AVNLLTSVIGDYGALDMRLMSRVLDNLLNNALRYSRTTVQVSLLLDGSQAALIVEDDGPG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDESELGGAKFSFH
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
WPVWHAMPDMTAA
CCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]