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The map label for this gene is bioD [H]

Identifier: 194736665

GI number: 194736665

Start: 919227

End: 919913

Strand: Direct

Name: bioD [H]

Synonym: SeSA_A0947

Alternate gene names: 194736665

Gene position: 919227-919913 (Clockwise)

Preceding gene: 194737115

Following gene: 194735904

Centisome position: 19.52

GC content: 58.81

Gene sequence:

>687_bases
GTGACTAAACGCTATTTTGTGACCGGTACTGATACTGAAGTCGGTAAAACGGTTGCCAGTTGCGCCTTATTGCAGGCCGC
AACGCAGTTAGGTTATCAAACCGTAGGCTATAAGCCCGTCGCTTCCGGCAGCGAAATGACAACCGACGGGCTACGCAATA
GCGATGCCCTGGCCTTGCAGCGTAACAGCAGCCTCCCGCAACCTTATTCCGCTATCAATCCTTATACTTTTGCCGAGCCT
ACCTCTCCCCACATCGTTAGCGCCGACGAAGGCCGGGCGATAGACGCGGCGGTATTATCACGCGGATTGCGAACGCTGGA
GGCGCAGGCAGACTGGGTGTTGACAGAAGGGGCGGGCGGCTGGTTTACACCGCTCTCTGCCACGCTGACGTTTGCCGACT
GGGTACAGACGGAGCAGCTGCCGGTGATTCTGGTTGTTGGCGTCAAGCTGGGCTGTATTAATCACGCCATGCTGACGGCA
CTGGCGGTAGAACAGGCCGGATTACCACTTGTCGGATGGATAGCCAACGACATACAGCCGCCGGGCGCGCGTCATGGTGA
ATATCTCGCGACGCTTCGCCGCGTCATACCGGCCCCGCTGTTAGGGGAAATCCCGTGGCTGGGCGTTTCTCCATCGCAGG
CGGCGACAGGACAGTATCTGGACCTTAGCCCCCTGGAACGCGCCTGA

Upstream 100 bases:

>100_bases
GTATTAACCCGCGCCCGGCTTAGACAGATTCAACTGGCATGGCCGCAGCGGCAGGGGAAATATCCGCTGACGTACCATCT
TTTTATGGGAGTGATTGAAC

Downstream 100 bases:

>100_bases
CCGCGCTTAAAAACCGCCTTCTTGCAGGATCTGACGATCCTGCTGTTCTTTCGCCAGAACCTCCTGACGTCTCCATGCTA
AAAATGTGCAAAATGTGAGC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEP
TSPHIVSADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTA
LAVEQAGLPLVGWIANDIQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA

Sequences:

>Translated_228_residues
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEP
TSPHIVSADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTA
LAVEQAGLPLVGWIANDIQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
>Mature_227_residues
TKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQRNSSLPQPYSAINPYTFAEPT
SPHIVSADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGGWFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTAL
AVEQAGLPLVGWIANDIQPPGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=75.1111111111111, Blast_Score=354, Evalue=2e-99,
Organism=Escherichia coli, GI87081944, Length=225, Percent_Identity=47.5555555555556, Blast_Score=205, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24200; Mature: 24069

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCEEEE
RNSSLPQPYSAINPYTFAEPTSPHIVSADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGG
CCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
WFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTALAVEQAGLPLVGWIANDIQP
EECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCC
PGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
CCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
TKRYFVTGTDTEVGKTVASCALLQAATQLGYQTVGYKPVASGSEMTTDGLRNSDALALQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCEEEE
RNSSLPQPYSAINPYTFAEPTSPHIVSADEGRAIDAAVLSRGLRTLEAQADWVLTEGAGG
CCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
WFTPLSATLTFADWVQTEQLPVILVVGVKLGCINHAMLTALAVEQAGLPLVGWIANDIQP
EECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCC
PGARHGEYLATLRRVIPAPLLGEIPWLGVSPSQAATGQYLDLSPLERA
CCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]