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The map label for this gene is mltD [H]

Identifier: 194734347

GI number: 194734347

Start: 301930

End: 303150

Strand: Reverse

Name: mltD [H]

Synonym: SeSA_A0290

Alternate gene names: 194734347

Gene position: 303150-301930 (Counterclockwise)

Preceding gene: 194736047

Following gene: 194737606

Centisome position: 6.44

GC content: 51.92

Gene sequence:

>1221_bases
ATGGACGACGGAACGTCTATCGCGCCAGATCAGGACTTGTGGGCTTTCATTGGCGACGAGCTAAAGATGGGAATTCCGGA
AAACAGCCGGATTCGCGAACAGAAACAGAAGTATTTACGCAATAAGAGCTATCTCCACGATGTAACTTTACGGGCAGAGC
CGTATATGTACTGGATAGCGGGGCAGGTTAAGAAACGTAACATGCCTATGGAGCTGGTACTACTACCCATAGTGGAGAGC
GCTTTTGATCCTCACGCAACGTCTGGCGCCAATGCCGCAGGCATCTGGCAGATCATACCGAGCACAGGGCGCAATTATGG
TTTAAAGCAGACCCGTAATTATGACGCGCGTCGCGATGTTGTGGCCTCTACGACCGCCGCGTTAGACATGATGCAGCGTC
TGAACAAAATGTTTGACGGCGACTGGCTGTTAACCGTTGCAGCGTATAACAGCGGCGAAGGCCGTGTTATGCGGGCAATT
AAAGCAAACAAAGCGCGTGGAAAACCAACGGATTTCTGGTCGCTTTCTTTGCCCAGGGAAACAAAACTCTACGTACCTAA
GATGCTGGCATTGAGTGAAATTCTCAAAAACAGCAAACGCTACGGCGTACGTCTGCCAACAACAGATGAAAGCCGAGCGC
TGGCTCGAGTTCGTTTAAACAGTCCAGTTGAAATGGCCCAGGTTGCGGATATGGCGGGCATGTCTATCAGCAAGCTGAAG
ACGTTTAATGCCGGTGTAAAAGGGTCTACCTTAGGCGTAAGCGGTCCGCAATATGTTATGGTGCCCAAAAAGCATGCCGA
GCAGTTGCGTGAGTCGCTGGCTTCCGGTGAGATTGACGCCGTTCAGTCCCAACTGATCGCCGACAATACGCCGTTGAATA
GCCGTAGCTATAAGGTGCGCTCTGGCGATACGCTTTCCGGTATTGCTTCGCGGCTTGGCGTAAGCACGAAGGACTTGCAG
CAGTGGAACAATCTGCGTGGTTCGCGCCTGAAAATCGGGCAAAGCCTGACCGTGGGCGCTGGCAGCAGCGCGCAACGACT
GGCAAACAATAGCGATAGCATTACCTATCGTGTCCGTAAAGGCGATTCGCTTTCCAGTATCGCCAGACGTCACGGGGTGA
ATATCAAAGATGTGATGCGCTGGAACCATGATACGGATAATCTACAACCGGGCGATCAGCTTACGTTGTTTGTGAAGGAC
AATAGCACGCCAGACTCCTGA

Upstream 100 bases:

>100_bases
CCAGACGTCTCAGAACACTGGCAACGTCCAACAGCACGCACAGAGCCTTTCTGCAGCTGGTCAAGGGGAAGCAGGAAAGT
TTACGAGTCAGGCACGATGG

Downstream 100 bases:

>100_bases
TATTGTCCGACGAAAAAGCCAAAAGGCGCCGCTTCCCCCGGCGCCTTTTAAATTGCTATACCGCTTTATGCGCTTCCAGC
ATGATAGTATCGCTGCTAAA

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MDDGTSIAPDQDLWAFIGDELKMGIPENSRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVES
AFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMRAI
KANKARGKPTDFWSLSLPRETKLYVPKMLALSEILKNSKRYGVRLPTTDESRALARVRLNSPVEMAQVADMAGMSISKLK
TFNAGVKGSTLGVSGPQYVMVPKKHAEQLRESLASGEIDAVQSQLIADNTPLNSRSYKVRSGDTLSGIASRLGVSTKDLQ
QWNNLRGSRLKIGQSLTVGAGSSAQRLANNSDSITYRVRKGDSLSSIARRHGVNIKDVMRWNHDTDNLQPGDQLTLFVKD
NSTPDS

Sequences:

>Translated_406_residues
MDDGTSIAPDQDLWAFIGDELKMGIPENSRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVES
AFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMRAI
KANKARGKPTDFWSLSLPRETKLYVPKMLALSEILKNSKRYGVRLPTTDESRALARVRLNSPVEMAQVADMAGMSISKLK
TFNAGVKGSTLGVSGPQYVMVPKKHAEQLRESLASGEIDAVQSQLIADNTPLNSRSYKVRSGDTLSGIASRLGVSTKDLQ
QWNNLRGSRLKIGQSLTVGAGSSAQRLANNSDSITYRVRKGDSLSSIARRHGVNIKDVMRWNHDTDNLQPGDQLTLFVKD
NSTPDS
>Mature_406_residues
MDDGTSIAPDQDLWAFIGDELKMGIPENSRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVES
AFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMRAI
KANKARGKPTDFWSLSLPRETKLYVPKMLALSEILKNSKRYGVRLPTTDESRALARVRLNSPVEMAQVADMAGMSISKLK
TFNAGVKGSTLGVSGPQYVMVPKKHAEQLRESLASGEIDAVQSQLIADNTPLNSRSYKVRSGDTLSGIASRLGVSTKDLQ
QWNNLRGSRLKIGQSLTVGAGSSAQRLANNSDSITYRVRKGDSLSSIARRHGVNIKDVMRWNHDTDNLQPGDQLTLFVKD
NSTPDS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=406, Percent_Identity=91.6256157635468, Blast_Score=770, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45020; Mature: 45020

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDGTSIAPDQDLWAFIGDELKMGIPENSRIREQKQKYLRNKSYLHDVTLRAEPYMYWIA
CCCCCCCCCCCHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEE
GQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDV
CHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
VASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMRAIKANKARGKPTDFWSLSLPRE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEECCCCC
TKLYVPKMLALSEILKNSKRYGVRLPTTDESRALARVRLNSPVEMAQVADMAGMSISKLK
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHH
TFNAGVKGSTLGVSGPQYVMVPKKHAEQLRESLASGEIDAVQSQLIADNTPLNSRSYKVR
HHCCCCCCCEECCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEC
SGDTLSGIASRLGVSTKDLQQWNNLRGSRLKIGQSLTVGAGSSAQRLANNSDSITYRVRK
CCCHHHHHHHHHCCCHHHHHHHHCCCCCEEECCCEEEECCCCHHHHHCCCCCCEEEEEEC
GDSLSSIARRHGVNIKDVMRWNHDTDNLQPGDQLTLFVKDNSTPDS
CCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MDDGTSIAPDQDLWAFIGDELKMGIPENSRIREQKQKYLRNKSYLHDVTLRAEPYMYWIA
CCCCCCCCCCCHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEE
GQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDV
CHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
VASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMRAIKANKARGKPTDFWSLSLPRE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEECCCCC
TKLYVPKMLALSEILKNSKRYGVRLPTTDESRALARVRLNSPVEMAQVADMAGMSISKLK
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHH
TFNAGVKGSTLGVSGPQYVMVPKKHAEQLRESLASGEIDAVQSQLIADNTPLNSRSYKVR
HHCCCCCCCEECCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEC
SGDTLSGIASRLGVSTKDLQQWNNLRGSRLKIGQSLTVGAGSSAQRLANNSDSITYRVRK
CCCHHHHHHHHHCCCHHHHHHHHCCCCCEEECCCEEEECCCCHHHHHCCCCCCEEEEEEC
GDSLSSIARRHGVNIKDVMRWNHDTDNLQPGDQLTLFVKDNSTPDS
CCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]