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The map label for this gene is gloB

Identifier: 194736047

GI number: 194736047

Start: 303369

End: 304124

Strand: Reverse

Name: gloB

Synonym: SeSA_A0291

Alternate gene names: 194736047

Gene position: 304124-303369 (Counterclockwise)

Preceding gene: 194736767

Following gene: 194734347

Centisome position: 6.46

GC content: 43.78

Gene sequence:

>756_bases
ATGAATCTTAACAGTATTCCCGCGTTTCAGGACAATTACATCTGGGTGCTGACCAATGATGAAGGCCGCTGTGTGATTGT
TGATCCCGGCGAAGCGGCACCCGTCTTAAAGGCCATTGCTGAACATAAGTGGATGCCGGAGGCGATTTTTCTGACGCATC
ACCATCACGACCATGTTGGCGGAGTCAAGGAGCTGTTGCAACACTTCCCGCAAATGACGGTTTATGGACCGGCGGAAACA
CAAGACAAGGGAGCAACCCATCTTGTTGGCGATGGCGATACTATTCGCGTTTTAGGCGAGAAATTTACTCTTTTTGCCAC
GCCGGGCCACACGTTAGGACACGTCTGTTACTTTAGCCATCCTTACTTATTCTGCGGCGACACGCTGTTCTCTGGCGGCT
GTGGTCGACTGTTTGAAGGCACGCCATCACAGATGTATCAGTCACTTATGAAAATTAATTCTCTGCCTGACGACACGCTC
ATTTGCTGCGCTCACGAATACACTTTAGCTAACATTAAGTTCGCATTGAGCATACTTCCGCACGATTCGTTCATAAATGA
ATATTATCGTAAAGTTAAAGAGTTACGTGTAAAAAAACAAATGACATTACCCGTTATTCTTAAAAATGAGCGTAAGATTA
ATCTTTTTTTAAGAACTGAAGATATTGATTTAATTAACGAAATAAACAAAGAAACAATATTGCAACAACCAGAAGCGCGT
TTTGCATGGTTAAGGTCAAAGAAAGACACGTTCTGA

Upstream 100 bases:

>100_bases
GCCCCAGGGCAACTCACCCCAACATCCGGGCGCAACGACAGTTTGAGAGAGCCTTGCCGGTTTCATCGCAACCTTCCGCT
AATTATTTGAGAGGTAATAT

Downstream 100 bases:

>100_bases
TAATTCTTACTTGTCATTCGCTAACTTCGCCGTTATGATCGGTCGTCTTTTAAGCAACTATTGACACACACATGAAGGCA
AAAGCGATATTACTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICCAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF

Sequences:

>Translated_251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICCAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF
>Mature_251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICCAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=33.0769230769231, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=33.0769230769231, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI14150041, Length=229, Percent_Identity=33.1877729257642, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=35.2422907488987, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21703352, Length=227, Percent_Identity=35.2422907488987, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=34.020618556701, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=78.4860557768924, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787158, Length=192, Percent_Identity=29.6875, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=253, Percent_Identity=30.4347826086957, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538952, Length=158, Percent_Identity=31.0126582278481, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=232, Percent_Identity=30.6034482758621, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=231, Percent_Identity=29.004329004329, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=34.8659003831418, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=34.8659003831418, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=34.8659003831418, Blast_Score=161, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SALA4 (B5F8X0)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002145261.1
- ProteinModelPortal:   B5F8X0
- SMR:   B5F8X0
- GeneID:   6795846
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B0305
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   PRK10241
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28614; Mature: 28614

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHH
GVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSH
HHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECEEEEEECCCCHHHEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEECC
HDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCHHHHHHCCHHHHHCCCCHH
FAWLRSKKDTF
HHHHHCCCCCC
>Mature Secondary Structure
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHH
GVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSH
HHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECEEEEEECCCCHHHEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEECC
HDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCHHHHHHCCHHHHHCCCCHH
FAWLRSKKDTF
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA