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The map label for this gene is nifU [H]

Identifier: 194334798

GI number: 194334798

Start: 2198662

End: 2199273

Strand: Reverse

Name: nifU [H]

Synonym: Paes_2001

Alternate gene names: 194334798

Gene position: 2199273-2198662 (Counterclockwise)

Preceding gene: 194334799

Following gene: 194334797

Centisome position: 87.52

GC content: 49.51

Gene sequence:

>612_bases
ATGTTGCAGCAAAATGACTGGGTATATACCGAGACACTCAAAGAGCATTTCATGAACCCGAAAAATATTCTCCAGGGAGA
GGATACCGACGCATTTGACGGTGTCGGTATGGAGGGCAATCTCTCCTGCGGAGACCAGATGATGGTTGTCATCAAGGTTG
ACCGGGATAAGGAGATCATTACGGACTGTCAGTGGAAAACCTATGGTTGTGCAAGTGCAATTGCCAGTACATCGGTTCTT
TCGGAAATGGTCAAGGGCATGACGCTCGATCAGGCGTTCGATGTCTCACCCAAAGATGTCACCAAAGAACTCGGCGGGCT
TCCTGAAAACAAGATCCATTGTTCGGTTCTTGGCGATAAGGCTCTTCGGGCAGCGATCAACAACTATTTCGAGCGCAACG
GTATGGCTGACAGGATGCGAAAGGAGCAGGCAAAAATTATCTGTCAGTGTATGAACGTGACCGATGCCGAAATCGAGGAA
GCCGTTCTCGAGGGGGCAAGGAGTTTCTATGAACTGCAGGAGCATACCAAGATCGGTACGGTCTGCGGTCAGTGCAAGGA
GAGTGCAGAAGGACTGATCGAACAGTACTGTCATCTGCATTTCGACCATTGA

Upstream 100 bases:

>100_bases
GAGACGACCATGGAGGAGATCGAGTATGTGCTCGATGTTCTGCCAGGGGTCATATCACGAATAAGAAGTATGTCAACAGC
TTATATAAAAGGAGAAGAAC

Downstream 100 bases:

>100_bases
CAGCGTCCGGCGCCACCAGCCAGAGGTAAACCTTCGGATACAGACAGAAAGAGCCGGCATAGCACGCTATGCCGGCTCTT
TCTGTCTGTATCACGACACT

Product: nitrogen-fixing NifU domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLQQNDWVYTETLKEHFMNPKNILQGEDTDAFDGVGMEGNLSCGDQMMVVIKVDRDKEIITDCQWKTYGCASAIASTSVL
SEMVKGMTLDQAFDVSPKDVTKELGGLPENKIHCSVLGDKALRAAINNYFERNGMADRMRKEQAKIICQCMNVTDAEIEE
AVLEGARSFYELQEHTKIGTVCGQCKESAEGLIEQYCHLHFDH

Sequences:

>Translated_203_residues
MLQQNDWVYTETLKEHFMNPKNILQGEDTDAFDGVGMEGNLSCGDQMMVVIKVDRDKEIITDCQWKTYGCASAIASTSVL
SEMVKGMTLDQAFDVSPKDVTKELGGLPENKIHCSVLGDKALRAAINNYFERNGMADRMRKEQAKIICQCMNVTDAEIEE
AVLEGARSFYELQEHTKIGTVCGQCKESAEGLIEQYCHLHFDH
>Mature_203_residues
MLQQNDWVYTETLKEHFMNPKNILQGEDTDAFDGVGMEGNLSCGDQMMVVIKVDRDKEIITDCQWKTYGCASAIASTSVL
SEMVKGMTLDQAFDVSPKDVTKELGGLPENKIHCSVLGDKALRAAINNYFERNGMADRMRKEQAKIICQCMNVTDAEIEE
AVLEGARSFYELQEHTKIGTVCGQCKESAEGLIEQYCHLHFDH

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=128, Percent_Identity=39.84375, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI24307953, Length=115, Percent_Identity=41.7391304347826, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1788878, Length=121, Percent_Identity=46.2809917355372, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17543474, Length=124, Percent_Identity=38.7096774193548, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325122, Length=122, Percent_Identity=40.9836065573771, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=39.344262295082, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21355597, Length=122, Percent_Identity=42.6229508196721, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 22769; Mature: 22769

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQQNDWVYTETLKEHFMNPKNILQGEDTDAFDGVGMEGNLSCGDQMMVVIKVDRDKEII
CCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
TDCQWKTYGCASAIASTSVLSEMVKGMTLDQAFDVSPKDVTKELGGLPENKIHCSVLGDK
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHCCCCCCCEEEEEHHHH
ALRAAINNYFERNGMADRMRKEQAKIICQCMNVTDAEIEEAVLEGARSFYELQEHTKIGT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VCGQCKESAEGLIEQYCHLHFDH
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQQNDWVYTETLKEHFMNPKNILQGEDTDAFDGVGMEGNLSCGDQMMVVIKVDRDKEII
CCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
TDCQWKTYGCASAIASTSVLSEMVKGMTLDQAFDVSPKDVTKELGGLPENKIHCSVLGDK
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHCCCCCCCEEEEEHHHH
ALRAAINNYFERNGMADRMRKEQAKIICQCMNVTDAEIEEAVLEGARSFYELQEHTKIGT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VCGQCKESAEGLIEQYCHLHFDH
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]