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The map label for this gene is miaB

Identifier: 194334797

GI number: 194334797

Start: 2196929

End: 2198254

Strand: Reverse

Name: miaB

Synonym: Paes_1999

Alternate gene names: 194334797

Gene position: 2198254-2196929 (Counterclockwise)

Preceding gene: 194334798

Following gene: 194334796

Centisome position: 87.48

GC content: 54.45

Gene sequence:

>1326_bases
ATGACAAAAAAGGTTTCTATCCGTACGTTCGGCTGTCAAATGAACCAGGCGGATACAGAAATTATTTCCTCTCTTCTTAC
GGCAGAAGGGTATTGCCTTGTTGCGGAGGAAGACGATGCCGATCTGGTGATGCTCAATACCTGCGCGGTACGGGAGAATG
CGGTCGAGAAAATTATGCACCATCTCGACAGCCTGAAGGGGCTTCGGCGTCGTCGCCGCGGACTTGTGGTGGGCGTGATC
GGATGCGTTCCCCAGTACTATCGTGAAGAGATGTTTCAGAAGTCCGGTGTGATCGATTTCATTGCCGGGCCTGACAGCTA
CCGTAAACTGCCGGGACTTATCCGTAACGCTTTTCAGGGGATCAGAGGGGCTGAACTCTTTTTGACGCAGAGTGAAACCT
ACGGCGATATCGAACCAATGCGCTCTGGTTCGATCAGCGCGTTTATTCCTGTGATGCGGGGGTGCAACAACATGTGCGCT
TTTTGTGTGGTTCCCTTTACCCGCGGGCGGGAGCGGAGCCGCTCGTTGCAGTCTGTTCTCGATGAGGTCCGGCGGCTTGA
AGGGCAGGGGTATCGTGAACTGACCCTTCTTGGACAGAATGTCAATTCCTACCGCGACGACGAAGCCGGGGCGGATTTTG
CTCTTCTTCTTGATGAGGTGAGCAAGGCGGCAGGGGAGATGAGGGTCCGCTTCACTACATCGCATCCGAAAGATATATCA
GAGGATCTGGTGAACGTGGTCGCTCAGCGGGAGAATGTCTGCAATGCCATTCATCTGCCGGTTCAGAGCGGTTCTTCACG
AATGCTTGAGGTCATGCATCGCGGCCATACACGCCGGGAGTACCTCGACAAGATTGCCATGATCCGCAGTGCCGTTCCCG
GTGTTGCGCTCTCCACCGACCTTATTGCCGGATTCTGCGGCGAAACAGAGGAGGATCATCTCCAGACGCTGAGCCTTATG
GAGGAGGTTCGTTTTGATTACGCCTATATGTTTTATTATTCCGTGCGTCCCGGAACGTATGCCGCCCGCCATCTTACAGA
CGATGTGGCGCTGGAAGAGAAAAAAAGGCGGCTGCAGGAGATTATCGATCTGCAGAGCGGCATTTCAGGGGAGATCTTCG
GCAATGATGTAGGAAGCGTTCAGGAGGTTCTTGCGGAATCAGAAAGTCGTCGTTCATCCGATATGCTGATGGGCCGTTCC
GATACCAACAGGGTTGTCGTTTTCGATCGACAGCACTATCAGCCGGGGGATCTTGTCCGTGTCGTTATCGAATCATCGAC
CCAGGCGACGCTCATCGGGCGCTGTCAGGATCCCGGGGAGGGGTGA

Upstream 100 bases:

>100_bases
TAGAGTACAGAGAAAGAGGTTAATGCTTTGAAAACTTTTGGCGTGCGGCTATACTACGATAGTTTGAATGCGTGAATGGG
TATAAAAATATGAATTACTG

Downstream 100 bases:

>100_bases
TTGTTGACTGCTTCACGTGTAACTTGAAGAATGAACCTTTTTTATCTTGAAGGGGATTTGTAAATTCAACTTTTTATGAA
GTAACCCTACAATGACATGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMHHLDSLKGLRRRRRGLVVGVI
GCVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQGIRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCA
FCVVPFTRGRERSRSLQSVLDEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDIS
EDLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTDLIAGFCGETEEDHLQTLSLM
EEVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQEIIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRS
DTNRVVVFDRQHYQPGDLVRVVIESSTQATLIGRCQDPGEG

Sequences:

>Translated_441_residues
MTKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMHHLDSLKGLRRRRRGLVVGVI
GCVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQGIRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCA
FCVVPFTRGRERSRSLQSVLDEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDIS
EDLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTDLIAGFCGETEEDHLQTLSLM
EEVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQEIIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRS
DTNRVVVFDRQHYQPGDLVRVVIESSTQATLIGRCQDPGEG
>Mature_440_residues
TKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMHHLDSLKGLRRRRRGLVVGVIG
CVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQGIRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCAF
CVVPFTRGRERSRSLQSVLDEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDISE
DLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTDLIAGFCGETEEDHLQTLSLME
EVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQEIIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRSD
TNRVVVFDRQHYQPGDLVRVVIESSTQATLIGRCQDPGEG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=43.5517970401691, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=43.5517970401691, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI93277076, Length=445, Percent_Identity=26.2921348314607, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=40.5405405405405, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=27.5784753363229, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=454, Percent_Identity=40.7488986784141, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI71996771, Length=303, Percent_Identity=24.0924092409241, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=39.873417721519, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI19922432, Length=447, Percent_Identity=27.7404921700224, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROA2 (B4S564)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016657.1
- ProteinModelPortal:   B4S564
- SMR:   B4S564
- GeneID:   6459858
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1999
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14340
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49396; Mature: 49265

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMH
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHH
HLDSLKGLRRRRRGLVVGVIGCVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQG
HHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCC
IRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCAFCVVPFTRGRERSRSLQSVL
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHH
DEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDIS
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHH
EDLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTD
HHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
LIAGFCGETEEDHLQTLSLMEEVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQE
HHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRSDTNRVVVFDRQHYQPGDLVR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEECCCCCCEEEEEECCCCCCCHHEE
VVIESSTQATLIGRCQDPGEG
EEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TKKVSIRTFGCQMNQADTEIISSLLTAEGYCLVAEEDDADLVMLNTCAVRENAVEKIMH
CCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHH
HLDSLKGLRRRRRGLVVGVIGCVPQYYREEMFQKSGVIDFIAGPDSYRKLPGLIRNAFQG
HHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCC
IRGAELFLTQSETYGDIEPMRSGSISAFIPVMRGCNNMCAFCVVPFTRGRERSRSLQSVL
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHH
DEVRRLEGQGYRELTLLGQNVNSYRDDEAGADFALLLDEVSKAAGEMRVRFTTSHPKDIS
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHH
EDLVNVVAQRENVCNAIHLPVQSGSSRMLEVMHRGHTRREYLDKIAMIRSAVPGVALSTD
HHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
LIAGFCGETEEDHLQTLSLMEEVRFDYAYMFYYSVRPGTYAARHLTDDVALEEKKRRLQE
HHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IIDLQSGISGEIFGNDVGSVQEVLAESESRRSSDMLMGRSDTNRVVVFDRQHYQPGDLVR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEECCCCCCEEEEEECCCCCCCHHEE
VVIESSTQATLIGRCQDPGEG
EEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA