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The map label for this gene is dagA [H]

Identifier: 194334670

GI number: 194334670

Start: 2043712

End: 2045286

Strand: Reverse

Name: dagA [H]

Synonym: Paes_1871

Alternate gene names: 194334670

Gene position: 2045286-2043712 (Counterclockwise)

Preceding gene: 194334671

Following gene: 194334669

Centisome position: 81.39

GC content: 49.97

Gene sequence:

>1575_bases
ATGGCTTTTTTCGGTCATATCAGCAGTAAAACAGCACACTTTGTTATGAAAAAAATTCTGTTAACTCTTGCCGGTCTGAT
CGGTATACTCTCTTTTCCTCTTCCGCTTCTGGCGGAAAAAGCTGTCGCTTCGGGTGTCGATCAGAGGATTAACGAAGCAA
TGGCACCGCTTACTGCTTTCATGTTCCGGATTGTTTTCTTTTCGGTACCGCTTGGCGGCGTGATGGTGCCGTTTGTCCTC
GTCTGGCTCATTGTTGCCGCCCTGGTTTTTACGGTGTACATGAAGTTCATCAATATCAGGGGCTTTGGCCATGCGATCGA
TCTTGTTCGGGGAGTGTATGATGCTCCCGACCAACAGGGGGGTGAAGTTTCGCATTTCCAGGCATTGACGGCAGCTCTTT
CGGCTACGGTCGGTTTAGGCAATATCGCCGGTGTCGCTGTGGCGATTACGCTTGGCGGGCCGGGGGCGACTTTCTGGATG
ATTATGGGAGGCCTATTCGGGATGTCATCGAAATTCGTAGAGTGCACTCTTGGTGTGAAGTATCGGAAGATCAATAGCAA
TGGCAGCGTTTCAGGCGGACCTATGTACTATCTCAGCAGGGGTTTTGCTGAAAAAGGGTTTCCGGGAGCTGGCAAGGTTC
TCGCTTTTTTCTTCGCTTTGATGTGTGTCGGAGGGTCTTTTGGGGGCGGCAATATGTTTCAGGCCAATCAGGCGTTCAGG
CAGACCGTCATCGCGACAGGAGGAGAGCAGAGCCTGCTTTTTGAGCAGGGGTGGGTTTTCGGACTGGTTATGGCTCTTCT
GGTCGGCGTTGTCATTATCGGGGGAATCAAGGGAATCGTGAGAGTCACCTCGAAGCTTGTTCCCGTGATGGTGGCTATTT
ATGTTTCTGCCTCCCTGTTCATTCTTTTTTCTTCTTTTGATCAGCTTCCAGCTGCCTTTTCGGCGATCGTAAACGGTGCG
TTTTCTCCTGAAGCGATCTACGGGGGTGTCATCGGTGTGCTTATACAGGGGTTCAAGCGGGCGGCATTTTCCAATGAAGC
GGGTATCGGTTCAGCACCGATAGCACACTCGGCAGTAAAAACAGATGAGCCGGTGACCGAAGGGCTTGTAGCTCTTCTTG
AACCGTTTCTCGATACCGTCGTGATCTGTACCATGACCGCTCTTGTTATTATTATCTCTGGGCTCTACATCGAGCAGGGA
AGTGACGGAATCGCCCTGACCTCGAGTGCTTTTGGACAGGCTATTTCGTGGTTCCCTTATATCCTGAGCGTTGCCGTACT
GCTCTTTGCTTTTTCTACCATGATATCCTGGTCATATTACGGTCTGAAAGCCTGGACCTATATTTTTGGTGAAAGCCGTC
TGGCCGATGTAAGTTACAAGCTTCTCTTCTGTATGTTCATCGTGATCGGTTCGGCGATGAACCTCGGGACGGTGATTGAT
TTTACCGATGCCATGATTTTTGCCATGTCGTTTCCCAATATTATTGGTTTGTATATTCTTGCTCCCACTGTCAGACGTGA
GCTTGACGACTATTTCCTCCGTCTTAACGAGGGGGAGATCAGGCGTTTCAGGTAA

Upstream 100 bases:

>100_bases
TACAAATGTAAGTACCGCGATCAAAGAATCCAAAAACTATTTTCTGTTTTTTTCTTTTTATATTAAAAAATTTGATTTTA
GCCGGCGGGCTCTGTCCCGT

Downstream 100 bases:

>100_bases
TGGGAAAATTGATTTGCGGATCGATATGTCATGAATTCCGAACTAAAAAGCCGGTTTTGTACTTGTAATTACTGTCTGTG
CTGTAAGGAGCTGAGGCTTG

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAFFGHISSKTAHFVMKKILLTLAGLIGILSFPLPLLAEKAVASGVDQRINEAMAPLTAFMFRIVFFSVPLGGVMVPFVL
VWLIVAALVFTVYMKFINIRGFGHAIDLVRGVYDAPDQQGGEVSHFQALTAALSATVGLGNIAGVAVAITLGGPGATFWM
IMGGLFGMSSKFVECTLGVKYRKINSNGSVSGGPMYYLSRGFAEKGFPGAGKVLAFFFALMCVGGSFGGGNMFQANQAFR
QTVIATGGEQSLLFEQGWVFGLVMALLVGVVIIGGIKGIVRVTSKLVPVMVAIYVSASLFILFSSFDQLPAAFSAIVNGA
FSPEAIYGGVIGVLIQGFKRAAFSNEAGIGSAPIAHSAVKTDEPVTEGLVALLEPFLDTVVICTMTALVIIISGLYIEQG
SDGIALTSSAFGQAISWFPYILSVAVLLFAFSTMISWSYYGLKAWTYIFGESRLADVSYKLLFCMFIVIGSAMNLGTVID
FTDAMIFAMSFPNIIGLYILAPTVRRELDDYFLRLNEGEIRRFR

Sequences:

>Translated_524_residues
MAFFGHISSKTAHFVMKKILLTLAGLIGILSFPLPLLAEKAVASGVDQRINEAMAPLTAFMFRIVFFSVPLGGVMVPFVL
VWLIVAALVFTVYMKFINIRGFGHAIDLVRGVYDAPDQQGGEVSHFQALTAALSATVGLGNIAGVAVAITLGGPGATFWM
IMGGLFGMSSKFVECTLGVKYRKINSNGSVSGGPMYYLSRGFAEKGFPGAGKVLAFFFALMCVGGSFGGGNMFQANQAFR
QTVIATGGEQSLLFEQGWVFGLVMALLVGVVIIGGIKGIVRVTSKLVPVMVAIYVSASLFILFSSFDQLPAAFSAIVNGA
FSPEAIYGGVIGVLIQGFKRAAFSNEAGIGSAPIAHSAVKTDEPVTEGLVALLEPFLDTVVICTMTALVIIISGLYIEQG
SDGIALTSSAFGQAISWFPYILSVAVLLFAFSTMISWSYYGLKAWTYIFGESRLADVSYKLLFCMFIVIGSAMNLGTVID
FTDAMIFAMSFPNIIGLYILAPTVRRELDDYFLRLNEGEIRRFR
>Mature_523_residues
AFFGHISSKTAHFVMKKILLTLAGLIGILSFPLPLLAEKAVASGVDQRINEAMAPLTAFMFRIVFFSVPLGGVMVPFVLV
WLIVAALVFTVYMKFINIRGFGHAIDLVRGVYDAPDQQGGEVSHFQALTAALSATVGLGNIAGVAVAITLGGPGATFWMI
MGGLFGMSSKFVECTLGVKYRKINSNGSVSGGPMYYLSRGFAEKGFPGAGKVLAFFFALMCVGGSFGGGNMFQANQAFRQ
TVIATGGEQSLLFEQGWVFGLVMALLVGVVIIGGIKGIVRVTSKLVPVMVAIYVSASLFILFSSFDQLPAAFSAIVNGAF
SPEAIYGGVIGVLIQGFKRAAFSNEAGIGSAPIAHSAVKTDEPVTEGLVALLEPFLDTVVICTMTALVIIISGLYIEQGS
DGIALTSSAFGQAISWFPYILSVAVLLFAFSTMISWSYYGLKAWTYIFGESRLADVSYKLLFCMFIVIGSAMNLGTVIDF
TDAMIFAMSFPNIIGLYILAPTVRRELDDYFLRLNEGEIRRFR

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=452, Percent_Identity=31.6371681415929, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 56056; Mature: 55925

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFFGHISSKTAHFVMKKILLTLAGLIGILSFPLPLLAEKAVASGVDQRINEAMAPLTAF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRIVFFSVPLGGVMVPFVLVWLIVAALVFTVYMKFINIRGFGHAIDLVRGVYDAPDQQG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
GEVSHFQALTAALSATVGLGNIAGVAVAITLGGPGATFWMIMGGLFGMSSKFVECTLGVK
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCE
YRKINSNGSVSGGPMYYLSRGFAEKGFPGAGKVLAFFFALMCVGGSFGGGNMFQANQAFR
EEEECCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
QTVIATGGEQSLLFEQGWVFGLVMALLVGVVIIGGIKGIVRVTSKLVPVMVAIYVSASLF
HHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILFSSFDQLPAAFSAIVNGAFSPEAIYGGVIGVLIQGFKRAAFSNEAGIGSAPIAHSAVK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHC
TDEPVTEGLVALLEPFLDTVVICTMTALVIIISGLYIEQGSDGIALTSSAFGQAISWFPY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHHH
ILSVAVLLFAFSTMISWSYYGLKAWTYIFGESRLADVSYKLLFCMFIVIGSAMNLGTVID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FTDAMIFAMSFPNIIGLYILAPTVRRELDDYFLRLNEGEIRRFR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure 
AFFGHISSKTAHFVMKKILLTLAGLIGILSFPLPLLAEKAVASGVDQRINEAMAPLTAF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]