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The map label for this gene is spsK [H]

Identifier: 194334529

GI number: 194334529

Start: 1882291

End: 1883181

Strand: Reverse

Name: spsK [H]

Synonym: Paes_1724

Alternate gene names: 194334529

Gene position: 1883181-1882291 (Counterclockwise)

Preceding gene: 194334530

Following gene: 194334528

Centisome position: 74.94

GC content: 55.78

Gene sequence:

>891_bases
ATGAATATTCTTGTTACCGGGTCGAAGGGGCAGTTGGGGTCGGAGATTCGTGATGCTGCTATGCTTGACGGGGGTTTGCG
GTTTTTTTTCTGCGATTTGGCGGAGCTTGATATCACTGATCGTGATGCGGTTGTGAGGATGTGTGAGCGTGACGGGATTG
ATGTGATTATCAATTGTGCGGCATATACGGCGGTTGACAGGGCTGAGGATGATGCGGATACTGCGATGAGGGTGAACCGG
GATGGGCCGGGTGTGCTGGCGGAGTGTGCGCGTGAGCGAGGTGCGCTCTTGTTGCAGGTGTCGACGGATTATGTGTTTAA
TGGTGAGTCTTCGGTTCCGTATCGTGAATGTGACGAGGTTTCTCCCCTTGGTGTGTATGGTCAGTCGAAATGGGAGGGCG
AGGAGCTGGTGCGGCGGTCGGGGGCGTCATATATGATTTTCAGGACGTCCTGGTTGTATTCGGCGCATGGTAATAATTTT
GTGAAGACGATGCTTCGGCTGGGGGCTGAGCGCGATGAATTGCGGGTGGTATTTGACCAGGTGGGTACGCCATGTTACGC
GGCTGATTTGGCTCGCGCGCTGATGCATGTTCTCGAGCGTGTGGAGCCGGGGGTGAACTATGCGGCAACCTATCATTTTT
CGAATGAAGGGGTGTGTTCGTGGTATGATTTTGCGGTGATGATCATGCGGCTGGGCGGGCTCGGGTGCCGTGTCGTTCCG
ATTGAGAGCAGCGAGTTTCCTGCGAAGGTGACACGTCCTCATTTCAGCGTGCTGAACAAAGGGAAGATCAAAGCGGATTG
GGGGGTGGATGTTCCGCACTGGATGGATGGGTTGGAGAGGATGATGCGAGCGCCTTTGGCGCGGGGAGGCGCTTCGCGCG
GTGACGCGTGA

Upstream 100 bases:

>100_bases
GAGTGAATGGTGGCGCCTTTGGCGCAGTGACGAGTGACAAGTGACGAATAACGGTTAACGGATAACAAATAACGAATTAA
CAACTGAATGATTTATTTCT

Downstream 100 bases:

>100_bases
CGCGTGACAAGGGGCAAGAGACGAGAGACGGGAGGCCGGGGCGCCTGCGGCGCGGTGACGAGTTGCGCTGTTGGTGCAAA
CGGTTTACCATTCACGGATA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNILVTGSKGQLGSEIRDAAMLDGGLRFFFCDLAELDITDRDAVVRMCERDGIDVIINCAAYTAVDRAEDDADTAMRVNR
DGPGVLAECARERGALLLQVSTDYVFNGESSVPYRECDEVSPLGVYGQSKWEGEELVRRSGASYMIFRTSWLYSAHGNNF
VKTMLRLGAERDELRVVFDQVGTPCYAADLARALMHVLERVEPGVNYAATYHFSNEGVCSWYDFAVMIMRLGGLGCRVVP
IESSEFPAKVTRPHFSVLNKGKIKADWGVDVPHWMDGLERMMRAPLARGGASRGDA

Sequences:

>Translated_296_residues
MNILVTGSKGQLGSEIRDAAMLDGGLRFFFCDLAELDITDRDAVVRMCERDGIDVIINCAAYTAVDRAEDDADTAMRVNR
DGPGVLAECARERGALLLQVSTDYVFNGESSVPYRECDEVSPLGVYGQSKWEGEELVRRSGASYMIFRTSWLYSAHGNNF
VKTMLRLGAERDELRVVFDQVGTPCYAADLARALMHVLERVEPGVNYAATYHFSNEGVCSWYDFAVMIMRLGGLGCRVVP
IESSEFPAKVTRPHFSVLNKGKIKADWGVDVPHWMDGLERMMRAPLARGGASRGDA
>Mature_296_residues
MNILVTGSKGQLGSEIRDAAMLDGGLRFFFCDLAELDITDRDAVVRMCERDGIDVIINCAAYTAVDRAEDDADTAMRVNR
DGPGVLAECARERGALLLQVSTDYVFNGESSVPYRECDEVSPLGVYGQSKWEGEELVRRSGASYMIFRTSWLYSAHGNNF
VKTMLRLGAERDELRVVFDQVGTPCYAADLARALMHVLERVEPGVNYAATYHFSNEGVCSWYDFAVMIMRLGGLGCRVVP
IESSEFPAKVTRPHFSVLNKGKIKADWGVDVPHWMDGLERMMRAPLARGGASRGDA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=246, Percent_Identity=27.6422764227642, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI33519455, Length=246, Percent_Identity=27.6422764227642, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=36.0824742268041, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32757; Mature: 32757

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGSKGQLGSEIRDAAMLDGGLRFFFCDLAELDITDRDAVVRMCERDGIDVIINCA
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHCCCCEEEEEEH
AYTAVDRAEDDADTAMRVNRDGPGVLAECARERGALLLQVSTDYVFNGESSVPYRECDEV
HHHHHHCCCCCCCHHEEECCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCHHHCCC
SPLGVYGQSKWEGEELVRRSGASYMIFRTSWLYSAHGNNFVKTMLRLGAERDELRVVFDQ
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
VGTPCYAADLARALMHVLERVEPGVNYAATYHFSNEGVCSWYDFAVMIMRLGGLGCRVVP
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
IESSEFPAKVTRPHFSVLNKGKIKADWGVDVPHWMDGLERMMRAPLARGGASRGDA
ECCCCCCCHHCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCHHHCCCCCCCCC
>Mature Secondary Structure
MNILVTGSKGQLGSEIRDAAMLDGGLRFFFCDLAELDITDRDAVVRMCERDGIDVIINCA
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHCCCCEEEEEEH
AYTAVDRAEDDADTAMRVNRDGPGVLAECARERGALLLQVSTDYVFNGESSVPYRECDEV
HHHHHHCCCCCCCHHEEECCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCHHHCCC
SPLGVYGQSKWEGEELVRRSGASYMIFRTSWLYSAHGNNFVKTMLRLGAERDELRVVFDQ
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
VGTPCYAADLARALMHVLERVEPGVNYAATYHFSNEGVCSWYDFAVMIMRLGGLGCRVVP
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
IESSEFPAKVTRPHFSVLNKGKIKADWGVDVPHWMDGLERMMRAPLARGGASRGDA
ECCCCCCCHHCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]