Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 194334528

GI number: 194334528

Start: 1880573

End: 1881598

Strand: Reverse

Name: rfbB [H]

Synonym: Paes_1723

Alternate gene names: 194334528

Gene position: 1881598-1880573 (Counterclockwise)

Preceding gene: 194334529

Following gene: 194334527

Centisome position: 74.88

GC content: 54.78

Gene sequence:

>1026_bases
ATGCATATACTTATTACGGGCGGGGCGGGGTTTATCGGGTCGCATGTGGTGCGGCGGTTTGTCAATGCGTATCCGGAGTA
CCGGATAACGAACCTTGATGCGTTGACCTATGCGGGTAATCTGGAGAACCTTCGAGATGTGGAGGATCGGGAGAATTACC
GGTTTGTGAAGGGTGATATTACGGATGGGGACGCGATGATGGCGCTGTTCCGTGAGGAGCAGTTCGACGGGGTGATTCAT
CTGGCGGCGGAGTCGCATGTGGATCGTTCGATTGCCAATCCGACGGCGTTTGTGATGACGAATGTGCTGGGGACGGTGAA
TCTGCTGAATGCGGCGCGTACAGCCTGGGCGGGAGCGTTTGAGGGGAAGCTGTTTTACCATATTTCGACGGATGAGGTGT
ATGGGACGCTGGGTTCTGCCGGGATGTTTACGGAGGAGACGGCGTATGATCCGCACAGTCCGTATTCAGCGTCGAAGGCG
TCGTCGGATCATTTTGTTCGTGCCTATCACGATACCTACGGGCTGCCGGTGGTGGTGAGCAACTGTTCGAATAATTACGG
AGCGAACCAGTTTCCGGAGAAGCTGATTCCGTTGTTCATCAATAATATCAGAAACCGCAAACCGCTGCCGGTCTATGGGA
AGGGCGAGAATGTGCGGGACTGGCTGTGGGTGGTTGATCATGCTGAGGCAATTGATGTGATTTTCCATAGTGGCAAGCAT
GGCGAGACCTATAATATCGGAGGGCATAACGAGTGGACGAATATTGATCTGATCCGTCTGCTGTGCGGGATTATGGATCG
CAAGCTCGCCCGGAGAGAGGGGGAGTCGGCAGAGCTGATTACCTATGTGACGGATCGCGCTGGTCATGATCTGCGTTATG
CGATTGATTCGGGGAAGCTGCAGCGAGAGCTCGGGTGGTCGCCATCGATTCGTTTCGAGGAGGGATTGGAGAAGACGGTG
GACTGGTACCTGGAAAACGGCCAGTGGCTGGAGCGTGTGACCTCGGGTGAGTATCAGCGCTATTGA

Upstream 100 bases:

>100_bases
TGTGGGCAAGCAGAAATATTTTCTTTACTGGTCACTGGTCACTGGTCACTGGTCACTGGTCACTGGTCACTGGTCACGAA
TAACGAATCAATACTCTTTG

Downstream 100 bases:

>100_bases
ATGGTGATTGGTGATTGGTGATTGGTGATTGGTGGGCTACCGGGATGTAGGTGCTTGATCTGTGCTCTTTGTGGGTAGCA
CTGTTTTTTTCGATTTGTGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MHILITGGAGFIGSHVVRRFVNAYPEYRITNLDALTYAGNLENLRDVEDRENYRFVKGDITDGDAMMALFREEQFDGVIH
LAAESHVDRSIANPTAFVMTNVLGTVNLLNAARTAWAGAFEGKLFYHISTDEVYGTLGSAGMFTEETAYDPHSPYSASKA
SSDHFVRAYHDTYGLPVVVSNCSNNYGANQFPEKLIPLFINNIRNRKPLPVYGKGENVRDWLWVVDHAEAIDVIFHSGKH
GETYNIGGHNEWTNIDLIRLLCGIMDRKLARREGESAELITYVTDRAGHDLRYAIDSGKLQRELGWSPSIRFEEGLEKTV
DWYLENGQWLERVTSGEYQRY

Sequences:

>Translated_341_residues
MHILITGGAGFIGSHVVRRFVNAYPEYRITNLDALTYAGNLENLRDVEDRENYRFVKGDITDGDAMMALFREEQFDGVIH
LAAESHVDRSIANPTAFVMTNVLGTVNLLNAARTAWAGAFEGKLFYHISTDEVYGTLGSAGMFTEETAYDPHSPYSASKA
SSDHFVRAYHDTYGLPVVVSNCSNNYGANQFPEKLIPLFINNIRNRKPLPVYGKGENVRDWLWVVDHAEAIDVIFHSGKH
GETYNIGGHNEWTNIDLIRLLCGIMDRKLARREGESAELITYVTDRAGHDLRYAIDSGKLQRELGWSPSIRFEEGLEKTV
DWYLENGQWLERVTSGEYQRY
>Mature_341_residues
MHILITGGAGFIGSHVVRRFVNAYPEYRITNLDALTYAGNLENLRDVEDRENYRFVKGDITDGDAMMALFREEQFDGVIH
LAAESHVDRSIANPTAFVMTNVLGTVNLLNAARTAWAGAFEGKLFYHISTDEVYGTLGSAGMFTEETAYDPHSPYSASKA
SSDHFVRAYHDTYGLPVVVSNCSNNYGANQFPEKLIPLFINNIRNRKPLPVYGKGENVRDWLWVVDHAEAIDVIFHSGKH
GETYNIGGHNEWTNIDLIRLLCGIMDRKLARREGESAELITYVTDRAGHDLRYAIDSGKLQRELGWSPSIRFEEGLEKTV
DWYLENGQWLERVTSGEYQRY

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=325, Percent_Identity=40, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI42516563, Length=350, Percent_Identity=24.5714285714286, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56237023, Length=355, Percent_Identity=26.4788732394366, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=355, Percent_Identity=26.4788732394366, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=355, Percent_Identity=26.4788732394366, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=55.0847457627119, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=54.4927536231884, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=25.6267409470752, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788366, Length=298, Percent_Identity=25.503355704698, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=35.474006116208, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=33.0246913580247, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=359, Percent_Identity=27.0194986072423, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=361, Percent_Identity=26.8698060941828, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.9393939393939, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=359, Percent_Identity=25.9052924791086, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=26.6106442577031, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=23.8670694864048, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38583; Mature: 38583

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILITGGAGFIGSHVVRRFVNAYPEYRITNLDALTYAGNLENLRDVEDRENYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHCCCCCCCEEEEECCC
TDGDAMMALFREEQFDGVIHLAAESHVDRSIANPTAFVMTNVLGTVNLLNAARTAWAGAF
CCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
EGKLFYHISTDEVYGTLGSAGMFTEETAYDPHSPYSASKASSDHFVRAYHDTYGLPVVVS
CCEEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
NCSNNYGANQFPEKLIPLFINNIRNRKPLPVYGKGENVRDWLWVVDHAEAIDVIFHSGKH
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEECCHHHEEHHEECCCC
GETYNIGGHNEWTNIDLIRLLCGIMDRKLARREGESAELITYVTDRAGHDLRYAIDSGKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCH
QRELGWSPSIRFEEGLEKTVDWYLENGQWLERVTSGEYQRY
HHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MHILITGGAGFIGSHVVRRFVNAYPEYRITNLDALTYAGNLENLRDVEDRENYRFVKGDI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHCCCCCCCEEEEECCC
TDGDAMMALFREEQFDGVIHLAAESHVDRSIANPTAFVMTNVLGTVNLLNAARTAWAGAF
CCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
EGKLFYHISTDEVYGTLGSAGMFTEETAYDPHSPYSASKASSDHFVRAYHDTYGLPVVVS
CCEEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
NCSNNYGANQFPEKLIPLFINNIRNRKPLPVYGKGENVRDWLWVVDHAEAIDVIFHSGKH
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEECCHHHEEHHEECCCC
GETYNIGGHNEWTNIDLIRLLCGIMDRKLARREGESAELITYVTDRAGHDLRYAIDSGKL
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCH
QRELGWSPSIRFEEGLEKTVDWYLENGQWLERVTSGEYQRY
HHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]