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The map label for this gene is ywkE [H]

Identifier: 194334390

GI number: 194334390

Start: 1726609

End: 1727502

Strand: Reverse

Name: ywkE [H]

Synonym: Paes_1585

Alternate gene names: 194334390

Gene position: 1727502-1726609 (Counterclockwise)

Preceding gene: 194334393

Following gene: 194334389

Centisome position: 68.74

GC content: 55.15

Gene sequence:

>894_bases
ATGCAGCAAGCAGACAAAGAGTGGAAGAGTATAGAGCTTCTTCAGACCACGACAGCCTTTTTTGAGGAGAAGCAGGTCAG
CGAGGCACGCTTAAGTGCTGAGCTGCTGCTGTGCCATGTGCTCAAGGCGAACCGTCTGCAGCTCTATCTTCAGCACAGTC
GTCCGGTCTATCCGGATGAGCTTGATGCATACCGGGCGTTGTGCAGGAAGCGCCTTCAGGGCTGGCCTGTGCAGTACCTT
ACCGGAGAACAGTACTTTTACGGCCGAGTGTTCAAGGTTGATCCCCGTGTGCTTATTCCAAGACCCGAAACGGAGCTGGT
GGTGGAGCATGCCATCGAGCGTCTTCGGGGTTGCGGCGGAGAGGGCAGCCAGCTGTCAATTCTCGATATAGGAACCGGCA
GCGGCTGTATCGCTGTGACGGCGGCGCTTCAATTGCCCGGTGCCAGGATTACGGCGATTGACTGTTCAGCCGAGGCCCTT
GATGTTGCCAGAGAGAACGCCAGAAGCTACGGGGTTGAGACGAGGATCCGGTTTCTTCAGGCAGATATGCTTGCTCCGGA
ATTTCTTGAGGACGATGAGGCGGCCTATGATCTGATCATTGCAAATCCGCCCTATATTCCTGATTCGGAGTGGGATGATC
TGCAGGCAGAGGTTCGGGAACATGAGCCAAGGGTGGCCTTGACCACTGCCAGCGGTCTGGAGTGCTATCGAGCAGTTGCC
GCCAGAGCACCGAGCCTTCTCTGCCAAAGCGGTATCTTGTGTTTTGAACTGCATGCCGAGGGCGCCGGGGCAGTTTCTGT
TCTTATGAAAGAGAACGGGTTTGGCGATATTCTGCTGCACAAAGATTACGGTGGTTATGACAGAATACTCTCGGCAATTC
GGGGAGGGTTATAG

Upstream 100 bases:

>100_bases
TGTACATCTACGGGATTTGAAGGTGGAAAACGGTCCTGGGGGGGCGTAGGGCAACGATGGGTAATGAACGGGTGATCCAC
CTCAAACTGATTATTATATC

Downstream 100 bases:

>100_bases
AAAGGAGAGCTGTGATAAAGAACGGATTGCAATTTATTGTTCTGCGTATGTGTTTACAATGGAAAAATAATGGTTCCCCA
CTGCTGCAACAGGTTCTTTT

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQQADKEWKSIELLQTTTAFFEEKQVSEARLSAELLLCHVLKANRLQLYLQHSRPVYPDELDAYRALCRKRLQGWPVQYL
TGEQYFYGRVFKVDPRVLIPRPETELVVEHAIERLRGCGGEGSQLSILDIGTGSGCIAVTAALQLPGARITAIDCSAEAL
DVARENARSYGVETRIRFLQADMLAPEFLEDDEAAYDLIIANPPYIPDSEWDDLQAEVREHEPRVALTTASGLECYRAVA
ARAPSLLCQSGILCFELHAEGAGAVSVLMKENGFGDILLHKDYGGYDRILSAIRGGL

Sequences:

>Translated_297_residues
MQQADKEWKSIELLQTTTAFFEEKQVSEARLSAELLLCHVLKANRLQLYLQHSRPVYPDELDAYRALCRKRLQGWPVQYL
TGEQYFYGRVFKVDPRVLIPRPETELVVEHAIERLRGCGGEGSQLSILDIGTGSGCIAVTAALQLPGARITAIDCSAEAL
DVARENARSYGVETRIRFLQADMLAPEFLEDDEAAYDLIIANPPYIPDSEWDDLQAEVREHEPRVALTTASGLECYRAVA
ARAPSLLCQSGILCFELHAEGAGAVSVLMKENGFGDILLHKDYGGYDRILSAIRGGL
>Mature_297_residues
MQQADKEWKSIELLQTTTAFFEEKQVSEARLSAELLLCHVLKANRLQLYLQHSRPVYPDELDAYRALCRKRLQGWPVQYL
TGEQYFYGRVFKVDPRVLIPRPETELVVEHAIERLRGCGGEGSQLSILDIGTGSGCIAVTAALQLPGARITAIDCSAEAL
DVARENARSYGVETRIRFLQADMLAPEFLEDDEAAYDLIIANPPYIPDSEWDDLQAEVREHEPRVALTTASGLECYRAVA
ARAPSLLCQSGILCFELHAEGAGAVSVLMKENGFGDILLHKDYGGYDRILSAIRGGL

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=302, Percent_Identity=33.112582781457, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=31.9702602230483, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082085, Length=212, Percent_Identity=34.9056603773585, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324266, Length=218, Percent_Identity=31.1926605504587, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582226, Length=259, Percent_Identity=30.5019305019305, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32986; Mature: 32986

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQADKEWKSIELLQTTTAFFEEKQVSEARLSAELLLCHVLKANRLQLYLQHSRPVYPDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
LDAYRALCRKRLQGWPVQYLTGEQYFYGRVFKVDPRVLIPRPETELVVEHAIERLRGCGG
HHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCC
EGSQLSILDIGTGSGCIAVTAALQLPGARITAIDCSAEALDVARENARSYGVETRIRFLQ
CCCEEEEEEECCCCCCEEEEEEHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ADMLAPEFLEDDEAAYDLIIANPPYIPDSEWDDLQAEVREHEPRVALTTASGLECYRAVA
HHHCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHH
ARAPSLLCQSGILCFELHAEGAGAVSVLMKENGFGDILLHKDYGGYDRILSAIRGGL
HCCHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQQADKEWKSIELLQTTTAFFEEKQVSEARLSAELLLCHVLKANRLQLYLQHSRPVYPDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
LDAYRALCRKRLQGWPVQYLTGEQYFYGRVFKVDPRVLIPRPETELVVEHAIERLRGCGG
HHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCC
EGSQLSILDIGTGSGCIAVTAALQLPGARITAIDCSAEALDVARENARSYGVETRIRFLQ
CCCEEEEEEECCCCCCEEEEEEHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ADMLAPEFLEDDEAAYDLIIANPPYIPDSEWDDLQAEVREHEPRVALTTASGLECYRAVA
HHHCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHH
ARAPSLLCQSGILCFELHAEGAGAVSVLMKENGFGDILLHKDYGGYDRILSAIRGGL
HCCHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]