Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 194333831

GI number: 194333831

Start: 1108658

End: 1109266

Strand: Reverse

Name: thiE [H]

Synonym: Paes_1005

Alternate gene names: 194333831

Gene position: 1109266-1108658 (Counterclockwise)

Preceding gene: 194333832

Following gene: 194333830

Centisome position: 44.14

GC content: 53.04

Gene sequence:

>609_bases
ATGAATAAGCTGCTCTGCTTCATAACCACTCAGCAGGACAACCCCGAAATCGTTGCCGAAGAGGCGCTGAAAGGCGGGAC
CGGCATGATACAGTTACGGCATAAAAACGCTTCCGGCCACGACCTTTTCACCTGGGCCTTGTCGATCGGAAAGATGTGCC
GCAGGTATGGCGCGCTCTTCATCGTTAATGACCGGCTTGATATCGCTCTTGCCGCTGGTGCAGACGGCGTTCATCTCGGA
CAGAATGACCTCCCGATCGACGTTGCCCGGAAACTGCTCCCTTCTCCCGCTATAATAGGCTGTTCGGTCTCTTCTGTCCA
TGAAGCACTTGACGCGCAGCAAAAAGGAGCAGACTATATCGGACTGGGCCATATCTTTCCGACCGGCTCCAAACAAAAAG
AAAACGCCCCGCTCGGCCCTGAATATATCCGCACGGTAAAACAATCAGTTGATATTCCTCTTATCGCTATCGGCGGCATC
GGACTCGATAACGCACGAGAAGTCATGAGCAATGGCGCAGACGGTCTTGCTGTTATTTCTGCGATCAGCTCAGCCGTCAA
TCCCGCTGAAGCGGCTCGTGAGCTTGCAATCATCATTGAACAAACCTGA

Upstream 100 bases:

>100_bases
GAAGGGGCTTATGGCCTGGCAGCCATGTCGCTGTTTGCCTCACCGACCAATCTGCACAGAACCATCGAATCATTTCAAAC
TATTCTGAACCACGTGCATC

Downstream 100 bases:

>100_bases
TAGCCACATCCTTCTGATACCTACTCAACCATACTCTCCTTCTGAACGCATGAAAAAGAGCTACACCACAGTGCTGAGCA
TTGCAGGTTCCGATGGAAGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNKLLCFITTQQDNPEIVAEEALKGGTGMIQLRHKNASGHDLFTWALSIGKMCRRYGALFIVNDRLDIALAAGADGVHLG
QNDLPIDVARKLLPSPAIIGCSVSSVHEALDAQQKGADYIGLGHIFPTGSKQKENAPLGPEYIRTVKQSVDIPLIAIGGI
GLDNAREVMSNGADGLAVISAISSAVNPAEAARELAIIIEQT

Sequences:

>Translated_202_residues
MNKLLCFITTQQDNPEIVAEEALKGGTGMIQLRHKNASGHDLFTWALSIGKMCRRYGALFIVNDRLDIALAAGADGVHLG
QNDLPIDVARKLLPSPAIIGCSVSSVHEALDAQQKGADYIGLGHIFPTGSKQKENAPLGPEYIRTVKQSVDIPLIAIGGI
GLDNAREVMSNGADGLAVISAISSAVNPAEAARELAIIIEQT
>Mature_202_residues
MNKLLCFITTQQDNPEIVAEEALKGGTGMIQLRHKNASGHDLFTWALSIGKMCRRYGALFIVNDRLDIALAAGADGVHLG
QNDLPIDVARKLLPSPAIIGCSVSSVHEALDAQQKGADYIGLGHIFPTGSKQKENAPLGPEYIRTVKQSVDIPLIAIGGI
GLDNAREVMSNGADGLAVISAISSAVNPAEAARELAIIIEQT

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=169, Percent_Identity=35.5029585798817, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325042, Length=201, Percent_Identity=33.8308457711443, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21333; Mature: 21333

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLLCFITTQQDNPEIVAEEALKGGTGMIQLRHKNASGHDLFTWALSIGKMCRRYGALF
CCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEE
IVNDRLDIALAAGADGVHLGQNDLPIDVARKLLPSPAIIGCSVSSVHEALDAQQKGADYI
EEECCEEEEEECCCCCEEECCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEE
GLGHIFPTGSKQKENAPLGPEYIRTVKQSVDIPLIAIGGIGLDNAREVMSNGADGLAVIS
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCHHHHHH
AISSAVNPAEAARELAIIIEQT
HHHHCCCHHHHHHEEEEEEECC
>Mature Secondary Structure
MNKLLCFITTQQDNPEIVAEEALKGGTGMIQLRHKNASGHDLFTWALSIGKMCRRYGALF
CCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEE
IVNDRLDIALAAGADGVHLGQNDLPIDVARKLLPSPAIIGCSVSSVHEALDAQQKGADYI
EEECCEEEEEECCCCCEEECCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEE
GLGHIFPTGSKQKENAPLGPEYIRTVKQSVDIPLIAIGGIGLDNAREVMSNGADGLAVIS
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCHHHHHH
AISSAVNPAEAARELAIIIEQT
HHHHCCCHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]