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The map label for this gene is thiD [H]

Identifier: 194333830

GI number: 194333830

Start: 1107781

End: 1108608

Strand: Reverse

Name: thiD [H]

Synonym: Paes_1004

Alternate gene names: 194333830

Gene position: 1108608-1107781 (Counterclockwise)

Preceding gene: 194333831

Following gene: 194333828

Centisome position: 44.12

GC content: 53.74

Gene sequence:

>828_bases
ATGAAAAAGAGCTACACCACAGTGCTGAGCATTGCAGGTTCCGATGGAAGCGGAGGCGCCGGAATCCAGGCAGATCTGAA
AACAATTGCGGCCCTGGGCTGTTACGGATTGAGCGCCATCACTGCGCTGACGGCACAGAACACCTGCGACGTTGAATCGT
TCACAGCGGTAGAGCCGGAATTCATCAGCCATCAAATAGATGTCCTGAATAAAGACATCCGGATCGATGCCATCAAGATC
GGCATGCTCGGGAGTCAAAAAGCGATAGGAAGCATTGCCTGCAGCATCCGTAAAATGAAGAGCCTCCGGCCGATCGTACT
CGACACCGTACTCATCTCATCAAGCGGCAGCCATCTACTCCCCGAAGAAAGTATCGATGAACTCAAGCGCGAGCTCTTTC
CTCTCGCCCGGCTCATCACTCCAAACCTTCCCGAAGCATCGAAACTGACCGGCCGAACCGAACTCCCCTCATCAAAAACC
GAAATCCGCAAGGTTGCCGAAAAACTTCTGATAACAGGCCCTGAGGCCGTCCTGGTCAAAGGCGGGCATATGCAGGGAGA
TCGCTGTGAAGACCTGCTTTTGAGCGGTAGAGAGGAACGGTGGTTTTCCTCACCAAGGATCAGCACTGTCAATACCCACG
GCACAGGCTGTACCCTTTCATCAGCGATCGCTTGCTTTCTTGCAAAAGGCAGAGGGCTTGAAGACGCGGTTGCAGAAGGC
AGGAACTATCTTCTCGGAGCACTTGAAGCCGGATCATTTTTCCATCTGGGAAAAGGATCCGGGCCTCTTGATCACCTCTA
CCGGTACCGGCAGATGCCGGACCGGTAA

Upstream 100 bases:

>100_bases
AATCCCGCTGAAGCGGCTCGTGAGCTTGCAATCATCATTGAACAAACCTGATAGCCACATCCTTCTGATACCTACTCAAC
CATACTCTCCTTCTGAACGC

Downstream 100 bases:

>100_bases
CTGGTCTGCATGCTTAGAGCGACAGAAGCAGGAGTGACTCAGGCATTACACCTTGGGAGCCGTCCTATAGAGTAGTAGGT
GAAGCCAAGCTGCTCCATCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKKSYTTVLSIAGSDGSGGAGIQADLKTIAALGCYGLSAITALTAQNTCDVESFTAVEPEFISHQIDVLNKDIRIDAIKI
GMLGSQKAIGSIACSIRKMKSLRPIVLDTVLISSSGSHLLPEESIDELKRELFPLARLITPNLPEASKLTGRTELPSSKT
EIRKVAEKLLITGPEAVLVKGGHMQGDRCEDLLLSGREERWFSSPRISTVNTHGTGCTLSSAIACFLAKGRGLEDAVAEG
RNYLLGALEAGSFFHLGKGSGPLDHLYRYRQMPDR

Sequences:

>Translated_275_residues
MKKSYTTVLSIAGSDGSGGAGIQADLKTIAALGCYGLSAITALTAQNTCDVESFTAVEPEFISHQIDVLNKDIRIDAIKI
GMLGSQKAIGSIACSIRKMKSLRPIVLDTVLISSSGSHLLPEESIDELKRELFPLARLITPNLPEASKLTGRTELPSSKT
EIRKVAEKLLITGPEAVLVKGGHMQGDRCEDLLLSGREERWFSSPRISTVNTHGTGCTLSSAIACFLAKGRGLEDAVAEG
RNYLLGALEAGSFFHLGKGSGPLDHLYRYRQMPDR
>Mature_275_residues
MKKSYTTVLSIAGSDGSGGAGIQADLKTIAALGCYGLSAITALTAQNTCDVESFTAVEPEFISHQIDVLNKDIRIDAIKI
GMLGSQKAIGSIACSIRKMKSLRPIVLDTVLISSSGSHLLPEESIDELKRELFPLARLITPNLPEASKLTGRTELPSSKT
EIRKVAEKLLITGPEAVLVKGGHMQGDRCEDLLLSGREERWFSSPRISTVNTHGTGCTLSSAIACFLAKGRGLEDAVAEG
RNYLLGALEAGSFFHLGKGSGPLDHLYRYRQMPDR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=43.3460076045627, Blast_Score=215, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=39.7058823529412, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=34.6863468634686, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=38.6029411764706, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29485; Mature: 29485

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSYTTVLSIAGSDGSGGAGIQADLKTIAALGCYGLSAITALTAQNTCDVESFTAVEPE
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHH
FISHQIDVLNKDIRIDAIKIGMLGSQKAIGSIACSIRKMKSLRPIVLDTVLISSSGSHLL
HHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCC
PEESIDELKRELFPLARLITPNLPEASKLTGRTELPSSKTEIRKVAEKLLITGPEAVLVK
CHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GGHMQGDRCEDLLLSGREERWFSSPRISTVNTHGTGCTLSSAIACFLAKGRGLEDAVAEG
CCCCCCCHHHHHHHCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCC
RNYLLGALEAGSFFHLGKGSGPLDHLYRYRQMPDR
CCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKSYTTVLSIAGSDGSGGAGIQADLKTIAALGCYGLSAITALTAQNTCDVESFTAVEPE
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHH
FISHQIDVLNKDIRIDAIKIGMLGSQKAIGSIACSIRKMKSLRPIVLDTVLISSSGSHLL
HHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCC
PEESIDELKRELFPLARLITPNLPEASKLTGRTELPSSKTEIRKVAEKLLITGPEAVLVK
CHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GGHMQGDRCEDLLLSGREERWFSSPRISTVNTHGTGCTLSSAIACFLAKGRGLEDAVAEG
CCCCCCCHHHHHHHCCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCC
RNYLLGALEAGSFFHLGKGSGPLDHLYRYRQMPDR
CCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]