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The map label for this gene is thiE [C]

Identifier: 194333832

GI number: 194333832

Start: 1109259

End: 1109909

Strand: Reverse

Name: thiE [C]

Synonym: Paes_1006

Alternate gene names: 194333832

Gene position: 1109909-1109259 (Counterclockwise)

Preceding gene: 194333833

Following gene: 194333831

Centisome position: 44.17

GC content: 52.23

Gene sequence:

>651_bases
ATGCCGTCGCCAGGCCATGAACTTCCCCGGCTGTATATGGTCACATACAGCCGGGTTTTTTCTTTTCCCGAAGAAAAACT
CCCCTCCTTGGTATCAAGCGCAGTCTCCCGGTCGAGGATCATCGTTCAGCTTCGTGAAAAACACCTCGATACCGCCAGGC
TCTACACATTGGCGGCACGCCTGCAGTCAGCCATCAATAAAAGCGACTCACTGCTCTTTATCAATGAACGGAGCGACATC
GCACTGGCAGTTGGCGCCAACGGCGTGCACATGCCTGAACAAAGCTGCCCTCTCTCTGCAACACGCTCCATCTGTCCCGG
CTTGTACACCGGCAAAAGCACTCATTCAGTGCAATCCGCCATAACGGCAGAACAGGACGGTGCTGATTACCTGCTTTTCG
GTCCGGTATTCGATACGCCGCTCAAAAGAAAATACGGGCCTCCTCAGGGAGTAAAACAGCTCGGAGAGATCTGCAAAAAC
GTCTCAGTTCCTGTCTTCGCCATTGGAGGAATAACGCCAGAAAACGCAGCAAGTTGTCTCGATGAAGGGGCTTATGGCCT
GGCAGCCATGTCGCTGTTTGCCTCACCGACCAATCTGCACAGAACCATCGAATCATTTCAAACTATTCTGAACCACGTGC
ATCATGAATAA

Upstream 100 bases:

>100_bases
TGCCATGGGCGGATTCAGAATGGGTCCGATTTTCGGCGGCATGTTCCTCAGCGGCAAAAAGGTTGCCGGGCTTATTCTTG
AAGACATTCAGGGCTGATCT

Downstream 100 bases:

>100_bases
GCTGCTCTGCTTCATAACCACTCAGCAGGACAACCCCGAAATCGTTGCCGAAGAGGCGCTGAAAGGCGGGACCGGCATGA
TACAGTTACGGCATAAAAAC

Product: Thiamine-phosphate diphosphorylase

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MPSPGHELPRLYMVTYSRVFSFPEEKLPSLVSSAVSRSRIIVQLREKHLDTARLYTLAARLQSAINKSDSLLFINERSDI
ALAVGANGVHMPEQSCPLSATRSICPGLYTGKSTHSVQSAITAEQDGADYLLFGPVFDTPLKRKYGPPQGVKQLGEICKN
VSVPVFAIGGITPENAASCLDEGAYGLAAMSLFASPTNLHRTIESFQTILNHVHHE

Sequences:

>Translated_216_residues
MPSPGHELPRLYMVTYSRVFSFPEEKLPSLVSSAVSRSRIIVQLREKHLDTARLYTLAARLQSAINKSDSLLFINERSDI
ALAVGANGVHMPEQSCPLSATRSICPGLYTGKSTHSVQSAITAEQDGADYLLFGPVFDTPLKRKYGPPQGVKQLGEICKN
VSVPVFAIGGITPENAASCLDEGAYGLAAMSLFASPTNLHRTIESFQTILNHVHHE
>Mature_215_residues
PSPGHELPRLYMVTYSRVFSFPEEKLPSLVSSAVSRSRIIVQLREKHLDTARLYTLAARLQSAINKSDSLLFINERSDIA
LAVGANGVHMPEQSCPLSATRSICPGLYTGKSTHSVQSAITAEQDGADYLLFGPVFDTPLKRKYGPPQGVKQLGEICKNV
SVPVFAIGGITPENAASCLDEGAYGLAAMSLFASPTNLHRTIESFQTILNHVHHE

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=177, Percent_Identity=29.9435028248588, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 23465; Mature: 23334

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPGHELPRLYMVTYSRVFSFPEEKLPSLVSSAVSRSRIIVQLREKHLDTARLYTLAAR
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LQSAINKSDSLLFINERSDIALAVGANGVHMPEQSCPLSATRSICPGLYTGKSTHSVQSA
HHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
ITAEQDGADYLLFGPVFDTPLKRKYGPPQGVKQLGEICKNVSVPVFAIGGITPENAASCL
HHHCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
DEGAYGLAAMSLFASPTNLHRTIESFQTILNHVHHE
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSPGHELPRLYMVTYSRVFSFPEEKLPSLVSSAVSRSRIIVQLREKHLDTARLYTLAAR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LQSAINKSDSLLFINERSDIALAVGANGVHMPEQSCPLSATRSICPGLYTGKSTHSVQSA
HHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
ITAEQDGADYLLFGPVFDTPLKRKYGPPQGVKQLGEICKNVSVPVFAIGGITPENAASCL
HHHCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
DEGAYGLAAMSLFASPTNLHRTIESFQTILNHVHHE
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {hydroxymethylpyrimidine} 0.0004 {thiazole} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]