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The map label for this gene is mltD [H]

Identifier: 193214012

GI number: 193214012

Start: 331890

End: 333578

Strand: Direct

Name: mltD [H]

Synonym: Ctha_0293

Alternate gene names: 193214012

Gene position: 331890-333578 (Clockwise)

Preceding gene: 193214005

Following gene: 193214013

Centisome position: 10.08

GC content: 46.18

Gene sequence:

>1689_bases
ATGCTTAAATTGCCTTTCTTTCAGATGCTTGTTCGTTTGTTTTGCTTCTCTTGCCTTCCAATTCTGGCCGGTTGTGCTGC
AACGCAAACGCCGATTTCAATAGACGACTACCAACTCCATAACGACTGCAGGCTCTTTACTGCTCCGCCTAAAATCACAC
TATACCCTTCGCGCACTTGGGATGATCTTCGTGCTGCCCACAGACAAATTGCAGAAATTGACAGCACGCCTGCCCCTGCA
AACTTTGCTGACTCAACCGCCATTCAAATCGGTTATGCGCATATCCTATATCAGGCGGCCTTGAAAGCGCTTCAGCGCAC
ACCGATGCCGGATAAAGAAACCGCCCGCGAGGCTTTAACAGAAGCGCTTGAAATCATCTCCTCCGTGCTTGCACAACCTG
ATCTGAAGGCAGATCCGCAGCTCTCGCGACTTGCCTTGTACGTCGTCCAAACTTATGATGACCACATACAAAAACTCAGC
GAGCTTGAAGGCGATGCGCCAGCACTTCTCGTATATGAACGCTTGTTTGGCAACCCGGAAAATACCATCGTTGATGAAAA
CCTATTTCTCGGGATTTTGCTTCCTAAAACGGAAATTCCTCTTGAGCTCAATTATCAGGTTAAAAAGTTTATCACTTTTT
ATAGCTCTCGCTTTCACGATATTTTTCAGCGCTATTTAAATCGAGCTGAAATTTATTTTCCCATGATGCAAAAAATCATC
GAGGAAGAAAATGTCCCGCCGGAAATTATTTATTTAACCATTGTCGAAAGTGGTGTGAATCCACATGCTCGAAGCCATGC
AAACGCCGTTGGCGCATGGCAGTTTATTAAATCCACCGGAAGGCTTTTTGATTTGCACGGAAATAATTGGTTCGACGAAC
GCCAAGATATTGAAAAATCAACCCGAAGCGCCATGCGACTTTTAAAATCGCTACACCAGCGATATGGCGATTGGTATCTT
GCGCTTGCCGCCTATAATGCAGGAACGGGAAAAATTAACCGCGCTATTCGCCGTTCTGGGAAAAAGAACTTTTGGGAACT
AACTCGATACTTGAGAACTGAAACACGCCAGTATGTGGCCCGGTATATCGCCGCGTCCATTATTGCCATGCACCCTGAGC
ATTTTGGCTTCACCTCGCTCGAGTTTGAAGAAATTGAAGAAACTGAACTGGTTACCGTTCCCAATTGCCTTTCTCTTGAT
GTGCTTTCGCAATCGATCGGCATGCCCAAAGAACAATTGCAATTTTTAAATCCTGAACTTCGACAAGATGTAACACCGCC
GGCATATAAGAACTATCCGCTGCGCGTTCCCAAACGCTTGGCAAGCTCAGCCCAGCAGGCCATCGATTCCATTCCCGATT
CGGAGCAGCTTTTTTTCACACTCTATAAGCTCAAACAAAATGAGTCGCTTAGTCGGATTGCGAAAAAATTAGATGTCACG
CCATCCATATTGCAGGAAATTAATCACCTGAAGTCGAGTGTTGTTCCACGCGGAAAAGTGCTCATGATGCCAACTTCGCC
AGAGGCTTTTGCCGAAACCCATTTTTCGCGTCGTGAACTGTCTGATGATAGTCGCGAAAGAAGACGGCGTCGGCGTCGGT
ATAAACAACCGGCGTCGGAAGAAAATATCTCGCTGGTTCAAGTCTATCGGAAAATCCAAACCGATTTGCAAGCAGAGGAG
AAAGAGTAA

Upstream 100 bases:

>100_bases
TTTACTGCTTTTTAGGAACGAGAAAGATGCTCTATATTTTGCATCCATTCGCAGATATGCTATAAAATAGACACTACTTA
AAACTTTGATATATCAATTG

Downstream 100 bases:

>100_bases
ATGAACCGGCCATCTTTCGCCAATAGGCAAATCGTGGGCTTTGCCTTTAGCCTCGCAATGTCCATATTTCGGTTTTCTCA
CCTGTTACCGCTGTTTGGCT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MLKLPFFQMLVRLFCFSCLPILAGCAATQTPISIDDYQLHNDCRLFTAPPKITLYPSRTWDDLRAAHRQIAEIDSTPAPA
NFADSTAIQIGYAHILYQAALKALQRTPMPDKETAREALTEALEIISSVLAQPDLKADPQLSRLALYVVQTYDDHIQKLS
ELEGDAPALLVYERLFGNPENTIVDENLFLGILLPKTEIPLELNYQVKKFITFYSSRFHDIFQRYLNRAEIYFPMMQKII
EEENVPPEIIYLTIVESGVNPHARSHANAVGAWQFIKSTGRLFDLHGNNWFDERQDIEKSTRSAMRLLKSLHQRYGDWYL
ALAAYNAGTGKINRAIRRSGKKNFWELTRYLRTETRQYVARYIAASIIAMHPEHFGFTSLEFEEIEETELVTVPNCLSLD
VLSQSIGMPKEQLQFLNPELRQDVTPPAYKNYPLRVPKRLASSAQQAIDSIPDSEQLFFTLYKLKQNESLSRIAKKLDVT
PSILQEINHLKSSVVPRGKVLMMPTSPEAFAETHFSRRELSDDSRERRRRRRRYKQPASEENISLVQVYRKIQTDLQAEE
KE

Sequences:

>Translated_562_residues
MLKLPFFQMLVRLFCFSCLPILAGCAATQTPISIDDYQLHNDCRLFTAPPKITLYPSRTWDDLRAAHRQIAEIDSTPAPA
NFADSTAIQIGYAHILYQAALKALQRTPMPDKETAREALTEALEIISSVLAQPDLKADPQLSRLALYVVQTYDDHIQKLS
ELEGDAPALLVYERLFGNPENTIVDENLFLGILLPKTEIPLELNYQVKKFITFYSSRFHDIFQRYLNRAEIYFPMMQKII
EEENVPPEIIYLTIVESGVNPHARSHANAVGAWQFIKSTGRLFDLHGNNWFDERQDIEKSTRSAMRLLKSLHQRYGDWYL
ALAAYNAGTGKINRAIRRSGKKNFWELTRYLRTETRQYVARYIAASIIAMHPEHFGFTSLEFEEIEETELVTVPNCLSLD
VLSQSIGMPKEQLQFLNPELRQDVTPPAYKNYPLRVPKRLASSAQQAIDSIPDSEQLFFTLYKLKQNESLSRIAKKLDVT
PSILQEINHLKSSVVPRGKVLMMPTSPEAFAETHFSRRELSDDSRERRRRRRRYKQPASEENISLVQVYRKIQTDLQAEE
KE
>Mature_562_residues
MLKLPFFQMLVRLFCFSCLPILAGCAATQTPISIDDYQLHNDCRLFTAPPKITLYPSRTWDDLRAAHRQIAEIDSTPAPA
NFADSTAIQIGYAHILYQAALKALQRTPMPDKETAREALTEALEIISSVLAQPDLKADPQLSRLALYVVQTYDDHIQKLS
ELEGDAPALLVYERLFGNPENTIVDENLFLGILLPKTEIPLELNYQVKKFITFYSSRFHDIFQRYLNRAEIYFPMMQKII
EEENVPPEIIYLTIVESGVNPHARSHANAVGAWQFIKSTGRLFDLHGNNWFDERQDIEKSTRSAMRLLKSLHQRYGDWYL
ALAAYNAGTGKINRAIRRSGKKNFWELTRYLRTETRQYVARYIAASIIAMHPEHFGFTSLEFEEIEETELVTVPNCLSLD
VLSQSIGMPKEQLQFLNPELRQDVTPPAYKNYPLRVPKRLASSAQQAIDSIPDSEQLFFTLYKLKQNESLSRIAKKLDVT
PSILQEINHLKSSVVPRGKVLMMPTSPEAFAETHFSRRELSDDSRERRRRRRRYKQPASEENISLVQVYRKIQTDLQAEE
KE

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=375, Percent_Identity=29.8666666666667, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 64733; Mature: 64733

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLPFFQMLVRLFCFSCLPILAGCAATQTPISIDDYQLHNDCRLFTAPPKITLYPSRTW
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEECCCCEEECCCCCH
DDLRAAHRQIAEIDSTPAPANFADSTAIQIGYAHILYQAALKALQRTPMPDKETAREALT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EALEIISSVLAQPDLKADPQLSRLALYVVQTYDDHIQKLSELEGDAPALLVYERLFGNPE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
NTIVDENLFLGILLPKTEIPLELNYQVKKFITFYSSRFHDIFQRYLNRAEIYFPMMQKII
CCEECCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEENVPPEIIYLTIVESGVNPHARSHANAVGAWQFIKSTGRLFDLHGNNWFDERQDIEKS
HHCCCCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
TRSAMRLLKSLHQRYGDWYLALAAYNAGTGKINRAIRRSGKKNFWELTRYLRTETRQYVA
HHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RYIAASIIAMHPEHFGFTSLEFEEIEETELVTVPNCLSLDVLSQSIGMPKEQLQFLNPEL
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHCCHHH
RQDVTPPAYKNYPLRVPKRLASSAQQAIDSIPDSEQLFFTLYKLKQNESLSRIAKKLDVT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
PSILQEINHLKSSVVPRGKVLMMPTSPEAFAETHFSRRELSDDSRERRRRRRRYKQPASE
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
ENISLVQVYRKIQTDLQAEEKE
CCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKLPFFQMLVRLFCFSCLPILAGCAATQTPISIDDYQLHNDCRLFTAPPKITLYPSRTW
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEECCCCEEECCCCCH
DDLRAAHRQIAEIDSTPAPANFADSTAIQIGYAHILYQAALKALQRTPMPDKETAREALT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EALEIISSVLAQPDLKADPQLSRLALYVVQTYDDHIQKLSELEGDAPALLVYERLFGNPE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
NTIVDENLFLGILLPKTEIPLELNYQVKKFITFYSSRFHDIFQRYLNRAEIYFPMMQKII
CCEECCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEENVPPEIIYLTIVESGVNPHARSHANAVGAWQFIKSTGRLFDLHGNNWFDERQDIEKS
HHCCCCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
TRSAMRLLKSLHQRYGDWYLALAAYNAGTGKINRAIRRSGKKNFWELTRYLRTETRQYVA
HHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RYIAASIIAMHPEHFGFTSLEFEEIEETELVTVPNCLSLDVLSQSIGMPKEQLQFLNPEL
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHCCHHH
RQDVTPPAYKNYPLRVPKRLASSAQQAIDSIPDSEQLFFTLYKLKQNESLSRIAKKLDVT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
PSILQEINHLKSSVVPRGKVLMMPTSPEAFAETHFSRRELSDDSRERRRRRRRYKQPASE
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
ENISLVQVYRKIQTDLQAEEKE
CCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]