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The map label for this gene is proB [H]

Identifier: 193213198

GI number: 193213198

Start: 1698684

End: 1699769

Strand: Direct

Name: proB [H]

Synonym: Cpar_1553

Alternate gene names: 193213198

Gene position: 1698684-1699769 (Clockwise)

Preceding gene: 193213197

Following gene: 193213199

Centisome position: 74.2

GC content: 61.14

Gene sequence:

>1086_bases
ATGAGTCACCACCACCCGGTTTATAAACGAATCGTCGTCAAGGTCGGCACGAACGTCATCACAGGCCGCGACGGCAGGCT
CGACCCGGCCATCCTCGACAGCCTGACAGCGCAAATCGCAGCGCTGGCAAAGGACGGCGTGGAGGTGATTCTTGTCTCGT
CGGGCGCTGTAGGCGCCGGCCGGGGCATCGTGTCGCTCACCGGCGGGCTCACTCCGGTCGAGAAGCGGCAGGTGCTCGCC
GCCACCGGGCAGATCCGGCTCATCAACGCCTACAACGACCGCTTCGAAAAGCACGGAATGCTCGGCGCGCAACTCCTCGT
CACCAAGGGCGATTTCCGCGACCGCCAGCACTACCTCAACATGCGCACCTGCTTCGAGGCGCTGCTCGATCAGAAAATCG
TGCCCATTGTCAACGAGAACGATGCGGTGGCGGTCACTGAGCTGATGTTCACCGATAACGACGAACTCTCCGGGCTGATC
GCCTCGATGTTGCAGGTCGATGCGCACATCATCCTCTCCAACGTAGACGGACTGTTCGACATGCAGAGCGAAGATAATCC
GGTGATCGAGGAGATCGACCCCGGTTCGAAGAACTTCAGCCAGTACATCCGCCCCGGCAAATCGGAGTTCGGACGCGGCG
GCATGCTCACCAAGTGTAACATCGCTCACAAGCTCTCGCGGCTCGGCATCACCGTGCACATCGCCAACGGCACCGCTCCC
GGCATTTTGCAGACCATCGTGCAGGGCGAACGCGTCGGCACGAAATTCCTTGCCCAGAAAGCCAAGCACAGCCGCAAACG
CTGGGTCGCCCACAGCGAAGGGTTGGAAAAAGGAGCCGTCGTCATCAATCAGGGCGCCATCGACGCCCTCACCTCCCCCG
AACGTGCCACCAGCCTTCTACCCATCGGCATCACCGGAGTCGAAGGCTCATTCCAGCGCGGCGACGTCATCCGCATCTGC
TCGGAGAAGCGCGAAGTCATCGGCTACGGCATGGCCTCCTGCTCCGCCGAAAAAGCCAAATCCGCGATGGGGCAGAAAGG
CCACAAACCGGTAATTCACTACGACCACTTGTATCTGGTGCCGTAG

Upstream 100 bases:

>100_bases
GTTCGGCGAGGGAATGAACACGCTCAAGGTAGTCTCGCTGATTCTGGTGATCGTCGGCATTGCAGGTTTGCATTTCAGTC
AGGAAATCATGAAGTAAGGC

Downstream 100 bases:

>100_bases
AGGATCTTCGATGGGATTTTATGTGATTAGATGGAAAGGCAAGGACTCGATTTTTTAACCATCGACACATTCGGGAAATA
AACAATCTTTCGTAGGGGCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSHHHPVYKRIVVKVGTNVITGRDGRLDPAILDSLTAQIAALAKDGVEVILVSSGAVGAGRGIVSLTGGLTPVEKRQVLA
ATGQIRLINAYNDRFEKHGMLGAQLLVTKGDFRDRQHYLNMRTCFEALLDQKIVPIVNENDAVAVTELMFTDNDELSGLI
ASMLQVDAHIILSNVDGLFDMQSEDNPVIEEIDPGSKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTAP
GILQTIVQGERVGTKFLAQKAKHSRKRWVAHSEGLEKGAVVINQGAIDALTSPERATSLLPIGITGVEGSFQRGDVIRIC
SEKREVIGYGMASCSAEKAKSAMGQKGHKPVIHYDHLYLVP

Sequences:

>Translated_361_residues
MSHHHPVYKRIVVKVGTNVITGRDGRLDPAILDSLTAQIAALAKDGVEVILVSSGAVGAGRGIVSLTGGLTPVEKRQVLA
ATGQIRLINAYNDRFEKHGMLGAQLLVTKGDFRDRQHYLNMRTCFEALLDQKIVPIVNENDAVAVTELMFTDNDELSGLI
ASMLQVDAHIILSNVDGLFDMQSEDNPVIEEIDPGSKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTAP
GILQTIVQGERVGTKFLAQKAKHSRKRWVAHSEGLEKGAVVINQGAIDALTSPERATSLLPIGITGVEGSFQRGDVIRIC
SEKREVIGYGMASCSAEKAKSAMGQKGHKPVIHYDHLYLVP
>Mature_360_residues
SHHHPVYKRIVVKVGTNVITGRDGRLDPAILDSLTAQIAALAKDGVEVILVSSGAVGAGRGIVSLTGGLTPVEKRQVLAA
TGQIRLINAYNDRFEKHGMLGAQLLVTKGDFRDRQHYLNMRTCFEALLDQKIVPIVNENDAVAVTELMFTDNDELSGLIA
SMLQVDAHIILSNVDGLFDMQSEDNPVIEEIDPGSKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTAPG
ILQTIVQGERVGTKFLAQKAKHSRKRWVAHSEGLEKGAVVINQGAIDALTSPERATSLLPIGITGVEGSFQRGDVIRICS
EKREVIGYGMASCSAEKAKSAMGQKGHKPVIHYDHLYLVP

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=285, Percent_Identity=35.0877192982456, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI62912457, Length=283, Percent_Identity=34.982332155477, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1786437, Length=354, Percent_Identity=35.3107344632768, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17569967, Length=321, Percent_Identity=30.5295950155763, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=321, Percent_Identity=30.5295950155763, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=356, Percent_Identity=29.4943820224719, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=354, Percent_Identity=29.3785310734463, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21357643, Length=298, Percent_Identity=31.2080536912752, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39029; Mature: 38897

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHHPVYKRIVVKVGTNVITGRDGRLDPAILDSLTAQIAALAKDGVEVILVSSGAVGAG
CCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
RGIVSLTGGLTPVEKRQVLAATGQIRLINAYNDRFEKHGMLGAQLLVTKGDFRDRQHYLN
CCEEEECCCCCCHHHHHHEEECCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
MRTCFEALLDQKIVPIVNENDAVAVTELMFTDNDELSGLIASMLQVDAHIILSNVDGLFD
HHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCEE
MQSEDNPVIEEIDPGSKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTAP
CCCCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCCCEEEHHHHHHHHHCCEEEEECCCCCH
GILQTIVQGERVGTKFLAQKAKHSRKRWVAHSEGLEKGAVVINQGAIDALTSPERATSLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHEEE
PIGITGVEGSFQRGDVIRICSEKREVIGYGMASCSAEKAKSAMGQKGHKPVIHYDHLYLV
EEEEECCCCCCCCCCEEEEHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEECCEEEC
P
C
>Mature Secondary Structure 
SHHHPVYKRIVVKVGTNVITGRDGRLDPAILDSLTAQIAALAKDGVEVILVSSGAVGAG
CCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
RGIVSLTGGLTPVEKRQVLAATGQIRLINAYNDRFEKHGMLGAQLLVTKGDFRDRQHYLN
CCEEEECCCCCCHHHHHHEEECCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
MRTCFEALLDQKIVPIVNENDAVAVTELMFTDNDELSGLIASMLQVDAHIILSNVDGLFD
HHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCEE
MQSEDNPVIEEIDPGSKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTAP
CCCCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCCCEEEHHHHHHHHHCCEEEEECCCCCH
GILQTIVQGERVGTKFLAQKAKHSRKRWVAHSEGLEKGAVVINQGAIDALTSPERATSLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHEEE
PIGITGVEGSFQRGDVIRICSEKREVIGYGMASCSAEKAKSAMGQKGHKPVIHYDHLYLV
EEEEECCCCCCCCCCEEEEHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEECCEEEC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]