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The map label for this gene is ispF

Identifier: 193213173

GI number: 193213173

Start: 1672898

End: 1673371

Strand: Direct

Name: ispF

Synonym: Cpar_1528

Alternate gene names: 193213173

Gene position: 1672898-1673371 (Clockwise)

Preceding gene: 193213171

Following gene: 193213175

Centisome position: 73.08

GC content: 57.38

Gene sequence:

>474_bases
ATGCGCATAGGAATCGGAATAGACGTTCACCAGTTTTCAGAAGACCGGAAACTCATTGTCGGCGGGGTTGAAGTGCCCTC
CCCGGTCGGTCTTCTCGGCCACAGCGATGCCGATGTGCTGTTGCACGCCATCAGCGACGCCCTGCTCGGCGCGGCGGCTC
TCGGCGACATCGGCAAGCACTTCCCAGACACCAGCCCCGACTACAAGGACGCCGACAGCATGGAGCTACTGAAGCATGTC
GGCAAGCTGCTTGAACAAGAGGGGTACAAGCCGGTCAACATCGATACCATGCTGCTGCTCGAAAAGCCGAAAATCGCCCC
CTACATCGAACAGATGCGCCGCAATATCGCCAGCTGCCTCGACATGGACATGAACGACGTTTCGGTCAAGGCGACCACCA
ACGAAAAACTCGGCTACGTAGGCCGTCAGGAAGGCGCCTGCGCCCACGCAGTTTGTTTGATTGAAAAGAAATAA

Upstream 100 bases:

>100_bases
ATATAAACTTTGCCGAGCCGGCTCGTATGGTTCTGTATTTGAGTGTTTTGTCCTATCTTCAGCTACAGATTGTTTCACTC
ATTTGAATCAACACCATACC

Downstream 100 bases:

>100_bases
CTGAAGAGCACCTGTTTTTCAGCGACGTGAGCGGCTTGATGGCAAGTCGGACGGAGCGCAGCAAACGAGCCGCGCAACAG
CGGAAAACTGCGCTCACCCC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGIGIDVHQFSEDRKLIVGGVEVPSPVGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLKHV
GKLLEQEGYKPVNIDTMLLLEKPKIAPYIEQMRRNIASCLDMDMNDVSVKATTNEKLGYVGRQEGACAHAVCLIEKK

Sequences:

>Translated_157_residues
MRIGIGIDVHQFSEDRKLIVGGVEVPSPVGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLKHV
GKLLEQEGYKPVNIDTMLLLEKPKIAPYIEQMRRNIASCLDMDMNDVSVKATTNEKLGYVGRQEGACAHAVCLIEKK
>Mature_157_residues
MRIGIGIDVHQFSEDRKLIVGGVEVPSPVGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLKHV
GKLLEQEGYKPVNIDTMLLLEKPKIAPYIEQMRRNIASCLDMDMNDVSVKATTNEKLGYVGRQEGACAHAVCLIEKK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=54.4871794871795, Blast_Score=157, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLP8 (B3QPS3)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001999126.1
- GeneID:   6420470
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1528
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17050; Mature: 17050

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIGIDVHQFSEDRKLIVGGVEVPSPVGLLGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDYKDADSMELLKHVGKLLEQEGYKPVNIDTMLLLEKPKIAPYIEQMRRNIASCL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
DMDMNDVSVKATTNEKLGYVGRQEGACAHAVCLIEKK
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRIGIGIDVHQFSEDRKLIVGGVEVPSPVGLLGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDYKDADSMELLKHVGKLLEQEGYKPVNIDTMLLLEKPKIAPYIEQMRRNIASCL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
DMDMNDVSVKATTNEKLGYVGRQEGACAHAVCLIEKK
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA