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The map label for this gene is pfp [H]

Identifier: 193213175

GI number: 193213175

Start: 1674907

End: 1676112

Strand: Direct

Name: pfp [H]

Synonym: Cpar_1530

Alternate gene names: 193213175

Gene position: 1674907-1676112 (Clockwise)

Preceding gene: 193213173

Following gene: 193213176

Centisome position: 73.16

GC content: 60.12

Gene sequence:

>1206_bases
GTGAAAAAAATCGGTATCCTGACCAGCGGAGGCGACTGCGGCGGACTGAACGCCGTCATTAAGGGCGCCGCGCTCATGGC
CATGAACAAAGGCCTTGAACTCTATGTGATCCCCAACGGCTACGCCGGGCTGTACAACCTGCTGAATCAGGACCGCCTGG
TGAAGTTCGATGCTGCGCGTCTGGACCAGTTCCAGGCAAGTTTCGCCGGTTCGGAAGCAGGCCATTCGAGGGTCAAGATC
AAGGCGATCAGCAACCCCGACAAGTACAACCGCATCAAGAACGGTATGAAGAAGTTCGATCTCGACGCCCTCATCATCAG
CGGCGGCGACGACTCGGGCAGCGTCATGATCGATCTGAACCAGAACGGCATCCAGTGCATCCACTGCCCGAAGACGATGG
ACCTCGACTTGCAGACCTACTCGGTCGGCGGCGACTCCACCATCAACCGTATCGCCCAGTTCGTCGAGGATCTCAAAACC
ACCGGCCGCACGCACAACCGCGTGCTCGTCACCGAGGTGTTCGGCCGCTATGCCGGTCACACGGCATTCCGCAGCGGCGT
GGCTGCCGAGGCAGACTGCATCCTGATTCCGGAAATTCCAGTGGACTGGGATGTGGTGTACGAACACGTCGTCGAACGCT
TCACCCGCCGCATCCGCCAAAGCGACGTGCACAGCGGCACCTACACCATCGTGGTTGCCGAAGGCATGAAGAACGCCGAT
GGCACCGACATCGTCGATGAGTCGGCGGGCGTCGATGCGTTTGGCCACAAGAAGCTTGCTGGCGCGGGCAAGTACGTCTG
CCAGGAGATCAAAAAGCGCTTCAAGTCCGACCCCCGGATGCCGCTCTTCATGAAGGAGACCGGCATGTTCGTCGAGGGGA
TTTATGAAATTCCGGAGGTTCGCGAAGTGCATCCGGGCCACCTGGTGCGCGCCGGACACTCTTCGGCCTACGACATCAAC
TTCGGCTTCGAGGCTGGCGCGGCGGCGGTGCTGCTGCTGCTCGAAGGCAAAACCGGCGTCACCATCTCCAAGGTCAAGGG
GCGCAAGATCGAGTACATCGAATCATCCAAGGCAATCGAACAGCGCTACGTCGATCTGGACCAAGTCGCCATGTACGAAG
CGATCGGCACCTGCTTCGGTAGGCCCCCTGCCGAGTACGACCCGATTCCCCGCGAAGTGGACGGCGTCTACGAGCGGATT
TACTGA

Upstream 100 bases:

>100_bases
TCTATCTTGAAAACATACAATACGCCGTGCTTTCACTGGTGTATTTAATGAAATTAAGCCATCAAAACCTAACCCGAGCA
ACCAAGGAGGAGAGAGCATC

Downstream 100 bases:

>100_bases
GCAAACGCGGGCTGAGTTGACGTTGTGCATAAATCATACGTCAACTCAGCCCATACGTTCACTTCGGCTGATGCGCCGCC
CTTTTCTTCCCGCACTCAAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKIGILTSGGDCGGLNAVIKGAALMAMNKGLELYVIPNGYAGLYNLLNQDRLVKFDAARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKNGMKKFDLDALIISGGDDSGSVMIDLNQNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHVVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGVDAFGHKKLAGAGKYVCQEIKKRFKSDPRMPLFMKETGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIESSKAIEQRYVDLDQVAMYEAIGTCFGRPPAEYDPIPREVDGVYERI
Y

Sequences:

>Translated_401_residues
MKKIGILTSGGDCGGLNAVIKGAALMAMNKGLELYVIPNGYAGLYNLLNQDRLVKFDAARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKNGMKKFDLDALIISGGDDSGSVMIDLNQNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHVVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGVDAFGHKKLAGAGKYVCQEIKKRFKSDPRMPLFMKETGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIESSKAIEQRYVDLDQVAMYEAIGTCFGRPPAEYDPIPREVDGVYERI
Y
>Mature_401_residues
MKKIGILTSGGDCGGLNAVIKGAALMAMNKGLELYVIPNGYAGLYNLLNQDRLVKFDAARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKNGMKKFDLDALIISGGDDSGSVMIDLNQNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHVVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGVDAFGHKKLAGAGKYVCQEIKKRFKSDPRMPLFMKETGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIESSKAIEQRYVDLDQVAMYEAIGTCFGRPPAEYDPIPREVDGVYERI
Y

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI266453748, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4505749, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI266453619, Length=369, Percent_Identity=25.2032520325203, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI48762920, Length=378, Percent_Identity=25.3968253968254, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790350, Length=348, Percent_Identity=28.735632183908, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17558788, Length=406, Percent_Identity=27.3399014778325, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25147584, Length=363, Percent_Identity=24.7933884297521, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI72003601, Length=198, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652337, Length=407, Percent_Identity=25.0614250614251, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17647809, Length=407, Percent_Identity=25.0614250614251, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573326, Length=404, Percent_Identity=24.009900990099, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 44046; Mature: 44046

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAALMAMNKGLELYVIPNGYAGLYNLLNQDRLVKFDAAR
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHHHHHHCCCCEEEECHHH
LDQFQASFAGSEAGHSRVKIKAISNPDKYNRIKNGMKKFDLDALIISGGDDSGSVMIDLN
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEC
QNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKTTGRTHNRVLVTEVFGRYAGH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGVAAEADCILIPEIPVDWDVVYEHVVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIVDESAGVDAFGHKKLAGAGKYVCQEIKKRFKSDPRMPLFMKETGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCH
REVHPGHLVRAGHSSAYDINFGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIESSKAIE
HCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEHHHHHHHH
QRYVDLDQVAMYEAIGTCFGRPPAEYDPIPREVDGVYERIY
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAALMAMNKGLELYVIPNGYAGLYNLLNQDRLVKFDAAR
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHHHHHHCCCCEEEECHHH
LDQFQASFAGSEAGHSRVKIKAISNPDKYNRIKNGMKKFDLDALIISGGDDSGSVMIDLN
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEC
QNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKTTGRTHNRVLVTEVFGRYAGH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGVAAEADCILIPEIPVDWDVVYEHVVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIVDESAGVDAFGHKKLAGAGKYVCQEIKKRFKSDPRMPLFMKETGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCH
REVHPGHLVRAGHSSAYDINFGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIESSKAIE
HCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEHHHHHHHH
QRYVDLDQVAMYEAIGTCFGRPPAEYDPIPREVDGVYERIY
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]