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The map label for this gene is cca [H]

Identifier: 193212873

GI number: 193212873

Start: 1334160

End: 1335587

Strand: Direct

Name: cca [H]

Synonym: Cpar_1221

Alternate gene names: 193212873

Gene position: 1334160-1335587 (Clockwise)

Preceding gene: 193212872

Following gene: 193212874

Centisome position: 58.28

GC content: 60.43

Gene sequence:

>1428_bases
ATGCTGAAAGCAAAGCACGACGACATTCCAGAGATTTTGTACCGCATCGGAGACCTCGCCGACCAGGCCGGTCTCCCCTG
CTACCTCGTGGGCGGTTACGTTCGCGATCTCATCATGCAGCGCCCCTGCACCGACATCGACATCATGGTGATCGGCGAAC
CGGTGCCGTTCGCCAAAGCGATTGCCGAGCAGCTTGGCGGCCGGAATTTCGTGCTATTCGAGCGCTTCCGCACCGCGCAG
ATCGAGCTGGACGATCCGGAGGCGGGCACCTTCAAGCTTGAAATCGTCGGCGCACGCAAGGAGAGCTATAACCCCGAATC
ACGCAAGCCGATCACCAGCATCGGCACGCTGGAGGATGACCTCTCACGCCGCGACTTCACGATCAACGCCCTCGCCCTGC
GCCTGAACCGGGAGCAACGCGGCGAGCTCGTCGATCTGTACGACGGTCAGCGCCACATCCGCGAAAAACTGCTCAAAACG
CCACTCGATCCGGAACAGACCTTTTCGGACGATCCCCTTCGCATGATGCGCGCCGCCCGCTTCGCCTGCCAGCTCGATTT
CAGGCTCGATGAGGCAACGCTCGAAGCGATGGAGGCGATGAGCAGCCGGATCCAGATCGTCTCGCGCGAGCGCGTCAGCC
ACGAATTCCTCAAAATCATGGCGGCCCCGAAGCCATCGATCGGGCTCAAAATTCTGCATTCGACGGGCCTGCTCAAGGAG
ATCATCCCGGAACTGGACGTTATGGCGGGCATCGAACAGGTGGATGGCCTCGGCCACAAGGATACGCTGTTCCACACCTT
TCAGGTGGTGGACAACCTCGCGGAGCATTCTGACAAACTTTGGCTGCGCGTGAGCGCTCTCTTCCACGACATCGCCAAGC
CGGTGACCAAGCGCTTCCACCTAGGCTCCGGTTGGACCTTCCACGGCCACGAGGCGGTGGGAGTCAAGCTGGTACGACGC
ATTTTCCGGGCGATGAAGTGGCCCATGGAACCGATGGAGTACGTGCAGAAGATGGTGCGGCTGCACCACCGCCCGATTCC
GCTGTCGAAGGACGAAATCACCGACTCGGCGGTGCGCCGCCTGATGTTCGATGCCGGGCAGGACCTTGACGACCTGATGA
CGCTCTGCCGCGCCGACGTCACCAGTAAAAATCCGCGCAAGGTGCAGCGGATCATGAAGAATTTCAACAACGTCGAAGCG
AAAATTGCCGAGGTTGGCGAAAAGGATCTGCTGGCGAAATGGCGTCCTCCCGTCAGCGGCACCGACATCATGGAGATGCT
GAACCTGCCGCAGGGCAAGCTGGTGGGCATCATCAAGTCGCGCATGGAGAACGCCATCATCGACGGCGATATTCCCTACG
ACCGCCAGGCCGCGCTGGATTTTGTAAGTGCGGTGTACCGGGAAATGGTGGAGAAGAGGGAGGCTTGA

Upstream 100 bases:

>100_bases
CCGTGCGTTGATGGTGGCGGGCAGAATCACCGATGAAATCCGGAAGAACACCGCTGACCTCGACTGAATCCGACCCGACG
AATACGATACCGACGACCGA

Downstream 100 bases:

>100_bases
AAACACGTACATCCGTCTCCTGACGACCTGGTTTTCCCTCCGCTCTTGCTTTACTTATATTTATTGACACACAACTTCCT
GAACCAAACATCACCCTACC

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLKAKHDDIPEILYRIGDLADQAGLPCYLVGGYVRDLIMQRPCTDIDIMVIGEPVPFAKAIAEQLGGRNFVLFERFRTAQ
IELDDPEAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNREQRGELVDLYDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFRLDEATLEAMEAMSSRIQIVSRERVSHEFLKIMAAPKPSIGLKILHSTGLLKE
IIPELDVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHLGSGWTFHGHEAVGVKLVRR
IFRAMKWPMEPMEYVQKMVRLHHRPIPLSKDEITDSAVRRLMFDAGQDLDDLMTLCRADVTSKNPRKVQRIMKNFNNVEA
KIAEVGEKDLLAKWRPPVSGTDIMEMLNLPQGKLVGIIKSRMENAIIDGDIPYDRQAALDFVSAVYREMVEKREA

Sequences:

>Translated_475_residues
MLKAKHDDIPEILYRIGDLADQAGLPCYLVGGYVRDLIMQRPCTDIDIMVIGEPVPFAKAIAEQLGGRNFVLFERFRTAQ
IELDDPEAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNREQRGELVDLYDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFRLDEATLEAMEAMSSRIQIVSRERVSHEFLKIMAAPKPSIGLKILHSTGLLKE
IIPELDVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHLGSGWTFHGHEAVGVKLVRR
IFRAMKWPMEPMEYVQKMVRLHHRPIPLSKDEITDSAVRRLMFDAGQDLDDLMTLCRADVTSKNPRKVQRIMKNFNNVEA
KIAEVGEKDLLAKWRPPVSGTDIMEMLNLPQGKLVGIIKSRMENAIIDGDIPYDRQAALDFVSAVYREMVEKREA
>Mature_475_residues
MLKAKHDDIPEILYRIGDLADQAGLPCYLVGGYVRDLIMQRPCTDIDIMVIGEPVPFAKAIAEQLGGRNFVLFERFRTAQ
IELDDPEAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNREQRGELVDLYDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFRLDEATLEAMEAMSSRIQIVSRERVSHEFLKIMAAPKPSIGLKILHSTGLLKE
IIPELDVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHLGSGWTFHGHEAVGVKLVRR
IFRAMKWPMEPMEYVQKMVRLHHRPIPLSKDEITDSAVRRLMFDAGQDLDDLMTLCRADVTSKNPRKVQRIMKNFNNVEA
KIAEVGEKDLLAKWRPPVSGTDIMEMLNLPQGKLVGIIKSRMENAIIDGDIPYDRQAALDFVSAVYREMVEKREA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=327, Percent_Identity=31.1926605504587, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081691, Length=328, Percent_Identity=26.5243902439024, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=255, Percent_Identity=30.9803921568627, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54162; Mature: 54162

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAKHDDIPEILYRIGDLADQAGLPCYLVGGYVRDLIMQRPCTDIDIMVIGEPVPFAKA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
IAEQLGGRNFVLFERFRTAQIELDDPEAGTFKLEIVGARKESYNPESRKPITSIGTLEDD
HHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHH
LSRRDFTINALALRLNREQRGELVDLYDGQRHIREKLLKTPLDPEQTFSDDPLRMMRAAR
HCCCCEEHHHHHEEECHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FACQLDFRLDEATLEAMEAMSSRIQIVSRERVSHEFLKIMAAPKPSIGLKILHSTGLLKE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCHHHH
IIPELDVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEE
LGSGWTFHGHEAVGVKLVRRIFRAMKWPMEPMEYVQKMVRLHHRPIPLSKDEITDSAVRR
CCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMFDAGQDLDDLMTLCRADVTSKNPRKVQRIMKNFNNVEAKIAEVGEKDLLAKWRPPVSG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
TDIMEMLNLPQGKLVGIIKSRMENAIIDGDIPYDRQAALDFVSAVYREMVEKREA
HHHHHHHCCCCCHHHHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKAKHDDIPEILYRIGDLADQAGLPCYLVGGYVRDLIMQRPCTDIDIMVIGEPVPFAKA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
IAEQLGGRNFVLFERFRTAQIELDDPEAGTFKLEIVGARKESYNPESRKPITSIGTLEDD
HHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHH
LSRRDFTINALALRLNREQRGELVDLYDGQRHIREKLLKTPLDPEQTFSDDPLRMMRAAR
HCCCCEEHHHHHEEECHHHCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FACQLDFRLDEATLEAMEAMSSRIQIVSRERVSHEFLKIMAAPKPSIGLKILHSTGLLKE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCHHHH
IIPELDVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEE
LGSGWTFHGHEAVGVKLVRRIFRAMKWPMEPMEYVQKMVRLHHRPIPLSKDEITDSAVRR
CCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMFDAGQDLDDLMTLCRADVTSKNPRKVQRIMKNFNNVEAKIAEVGEKDLLAKWRPPVSG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
TDIMEMLNLPQGKLVGIIKSRMENAIIDGDIPYDRQAALDFVSAVYREMVEKREA
HHHHHHHCCCCCHHHHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA