Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 193212874

GI number: 193212874

Start: 1335688

End: 1336992

Strand: Direct

Name: purB [H]

Synonym: Cpar_1222

Alternate gene names: 193212874

Gene position: 1335688-1336992 (Clockwise)

Preceding gene: 193212873

Following gene: 193212877

Centisome position: 58.35

GC content: 59.23

Gene sequence:

>1305_bases
TTGATACCACGTTACTCGCCGAAAGACATTTCGGCAATCTGGAGCGATGAAGCCAAATTCGAACGCTGGCTGCAACTTGA
AATCGCCGCCGTCGAGGCGCGCATGGAAGCCGGAATCGTCCCGTCCGACGCGCTTGCGACCATCAAAGAGAAAGCGAAGT
TCAACGTCGAGGAGATTCTCACCATCGAGCGCGAGACCAAGCACGACGTCATCGCGTTCCTGACCAACGTCGCCGGATAC
GTCGGCCCCGACTCGCGTTACGTCCACGAGGGACTCACCTCTTCGGACGTGGTCGATACCTGCCTGGCCATGCAAATGCG
CGACGCAGGCAAGATCATCATCGCAGATATCGAGTCACTGATCGTTGTTCTTGGCAAGAAAGCCGTCGAGCACAAATACA
CCCTGCAGATGGGCCGCACGCACGGCATCCACGCCGAGCCGACCACCTTTGGCTTGAAGCTCTTGCTCTGGCACGAGGAG
ATGAAGCGCAACCTCGAACGCATGAAGCGCGCGCTCGAAACCGTTTCGGTCGGCAAAATTTCCGGCGCAGTCGGCACCTA
CCAGCACCTGTCACCGGACATCGAGGCTGCCGTCTGCGAGAAGCTCGGCCTGAAACCATCATCGATCTCGACGCAGATTC
TGCAGCGCGACCGACACGCCGAGTACGCCACGACACTCGCCATTATCGCCTCGTCGATCGAGAAGTTCTCGACCGAGCTG
CGCCACCTGCAGCGCACCGAGGTTCGCGAAACCGAAGAGTTCTTCAGCAAGGGCCAGAAAGGCAGCTCTGCGATGCCGCA
CAAGCGCAATCCGATCACTTTCGAGCGGCTCACCGGCCTGGCCCGCGTGGTGCGCTCCAACTCCATCGCCGCGATGGAGA
ACGTCGCTCTGTGGCACGAGCGCGACATTTCGCACTCCTCGGTCGAGCGTGTCATCATGCCCGACTCGACCATCGCGCTG
GTCTACATGCTGCGCACCTTCCGCGACTCGATCGAGACCTTGCTCGTCTATCCGGAACGCATGGAGCAGAACTTCGACAC
CTCCTACGGCCTTACCCTCTCGCAGACGCTGCTGCTCGCCCTCACCGGCAAAGGACTCACCCGCGAGGAGGCCTACCGCC
TCGTACAGCGCAACGCCATGAAGAGCTGGCAGGAGAAAATTCAGCTCAAGGAACTGGTGCTTCAGGACAAGGAGCTGCTC
GAACACATCACCGCCGAAGAGATCAACAAACTCTTCAGCCCGGAGACGATTCAGGGCAAGCTCAAAAACAGCGTGGATAT
TATTTTCAAGCGGAACGGACTCTGA

Upstream 100 bases:

>100_bases
AAACACGTACATCCGTCTCCTGACGACCTGGTTTTCCCTCCGCTCTTGCTTTACTTATATTTATTGACACACAACTTCCT
GAACCAAACATCACCCTACC

Downstream 100 bases:

>100_bases
TCGCTGACTTCTGACTGAATGAAAAACGAAAGGCCGATTGGTATTCCCACAATACCGACCGGCCTTTTCTGATTCCGATT
TTTGTATCAGCGCTCAGAAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPSDALATIKEKAKFNVEEILTIERETKHDVIAFLTNVAGY
VGPDSRYVHEGLTSSDVVDTCLAMQMRDAGKIIIADIESLIVVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWHEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCEKLGLKPSSISTQILQRDRHAEYATTLAIIASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMEQNFDTSYGLTLSQTLLLALTGKGLTREEAYRLVQRNAMKSWQEKIQLKELVLQDKELL
EHITAEEINKLFSPETIQGKLKNSVDIIFKRNGL

Sequences:

>Translated_434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPSDALATIKEKAKFNVEEILTIERETKHDVIAFLTNVAGY
VGPDSRYVHEGLTSSDVVDTCLAMQMRDAGKIIIADIESLIVVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWHEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCEKLGLKPSSISTQILQRDRHAEYATTLAIIASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMEQNFDTSYGLTLSQTLLLALTGKGLTREEAYRLVQRNAMKSWQEKIQLKELVLQDKELL
EHITAEEINKLFSPETIQGKLKNSVDIIFKRNGL
>Mature_434_residues
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPSDALATIKEKAKFNVEEILTIERETKHDVIAFLTNVAGY
VGPDSRYVHEGLTSSDVVDTCLAMQMRDAGKIIIADIESLIVVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWHEE
MKRNLERMKRALETVSVGKISGAVGTYQHLSPDIEAAVCEKLGLKPSSISTQILQRDRHAEYATTLAIIASSIEKFSTEL
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHERDISHSSVERVIMPDSTIAL
VYMLRTFRDSIETLLVYPERMEQNFDTSYGLTLSQTLLLALTGKGLTREEAYRLVQRNAMKSWQEKIQLKELVLQDKELL
EHITAEEINKLFSPETIQGKLKNSVDIIFKRNGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=445, Percent_Identity=27.6404494382022, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI183227688, Length=398, Percent_Identity=28.1407035175879, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1787376, Length=319, Percent_Identity=30.4075235109718, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=397, Percent_Identity=26.9521410579345, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32564234, Length=360, Percent_Identity=28.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=451, Percent_Identity=26.8292682926829, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647570, Length=440, Percent_Identity=27.0454545454545, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49147; Mature: 49147

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPSDALATIKEKAKFNVEEIL
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHH
TIERETKHDVIAFLTNVAGYVGPDSRYVHEGLTSSDVVDTCLAMQMRDAGKIIIADIESL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHH
IVVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWHEEMKRNLERMKRALETVSVGKI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPDIEAAVCEKLGLKPSSISTQILQRDRHAEYATTLAIIASSIEKFSTEL
CCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTIALVYMLRTFRDSIETLLVYPERMEQNFDTSYGLTLSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
LTGKGLTREEAYRLVQRNAMKSWQEKIQLKELVLQDKELLEHITAEEINKLFSPETIQGK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKNSVDIIFKRNGL
HCCCEEEEEECCCC
>Mature Secondary Structure
MIPRYSPKDISAIWSDEAKFERWLQLEIAAVEARMEAGIVPSDALATIKEKAKFNVEEIL
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHH
TIERETKHDVIAFLTNVAGYVGPDSRYVHEGLTSSDVVDTCLAMQMRDAGKIIIADIESL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHH
IVVLGKKAVEHKYTLQMGRTHGIHAEPTTFGLKLLLWHEEMKRNLERMKRALETVSVGKI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYQHLSPDIEAAVCEKLGLKPSSISTQILQRDRHAEYATTLAIIASSIEKFSTEL
CCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVRETEEFFSKGQKGSSAMPHKRNPITFERLTGLARVVRSNSIAAMENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTIALVYMLRTFRDSIETLLVYPERMEQNFDTSYGLTLSQTLLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
LTGKGLTREEAYRLVQRNAMKSWQEKIQLKELVLQDKELLEHITAEEINKLFSPETIQGK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKNSVDIIFKRNGL
HCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]