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The map label for this gene is thiE

Identifier: 193212772

GI number: 193212772

Start: 1235819

End: 1236433

Strand: Direct

Name: thiE

Synonym: Cpar_1118

Alternate gene names: 193212772

Gene position: 1235819-1236433 (Clockwise)

Preceding gene: 193212771

Following gene: 193212773

Centisome position: 53.98

GC content: 59.19

Gene sequence:

>615_bases
ATGACTATTCCACGACGACTGCTTTGCGTCATCACCGACCAGCAATCCAACCCTGTCGAACTGGCCCGCATGGCCCTCGA
AGGAGGCGCGCGCATGGTACAACTTCGAAGGAAATCGGCATCAGGCCGTGACCTTTTTGAATGGGCAGTCAGAATCCAGG
CGCTCTGCCGCGAATACGAAGCGTTGTTTATCGTTAACGACCGCGTAGACATTGCTCTTGCGGCACATGCCGACGGCGTG
CATCTCGGCCAGCAGGACATACCTGTTGCTTCGGCAAGAGCGCTGCTTGGCCCTGAAACGCTCATCGGCGTGTCGGTATC
AACCCCCTCCGAAGCCGCAAAAGCCGCAGAAGAAGGGGCCGATTACCTCGGCGTGGGGCACATCTTCCCCACCAGCTCCA
AGGAGAAACCGATGCCACCTATCGGTACGAGCGCGATCCGACCGATCATCGAGGCTTCTGGGCTGCCGGTCATCGCAATA
GGCGGCATCGAACTGCAGAACGTAGCTGAGGTCATCAAGGCCGGAGCATCAGGCGTTGCGGTCATCTCGGCAGTCTCAGG
TAGTGCCGACCCGGTTGCAGCGACCCGCGAACTGGTCAAACGCATCCGTCAATGA

Upstream 100 bases:

>100_bases
GCATTGAATGCGGAGCTTGGGGAGTTGCCGCCATGAGACCCTTTTTTGATCCAGAAACCATGCCTGAAACGATCAACCTC
TTCCTTTCGTATCTGCCCTC

Downstream 100 bases:

>100_bases
ACGCGACACGCGACTACACAACAGTACTGACCATCGCCGGTTCGGACGGCAGTGGCGGTGCAGGTATCCAGGCAGACCTG
AAAACCATCGCCGCGCATAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTIPRRLLCVITDQQSNPVELARMALEGGARMVQLRRKSASGRDLFEWAVRIQALCREYEALFIVNDRVDIALAAHADGV
HLGQQDIPVASARALLGPETLIGVSVSTPSEAAKAAEEGADYLGVGHIFPTSSKEKPMPPIGTSAIRPIIEASGLPVIAI
GGIELQNVAEVIKAGASGVAVISAVSGSADPVAATRELVKRIRQ

Sequences:

>Translated_204_residues
MTIPRRLLCVITDQQSNPVELARMALEGGARMVQLRRKSASGRDLFEWAVRIQALCREYEALFIVNDRVDIALAAHADGV
HLGQQDIPVASARALLGPETLIGVSVSTPSEAAKAAEEGADYLGVGHIFPTSSKEKPMPPIGTSAIRPIIEASGLPVIAI
GGIELQNVAEVIKAGASGVAVISAVSGSADPVAATRELVKRIRQ
>Mature_203_residues
TIPRRLLCVITDQQSNPVELARMALEGGARMVQLRRKSASGRDLFEWAVRIQALCREYEALFIVNDRVDIALAAHADGVH
LGQQDIPVASARALLGPETLIGVSVSTPSEAAKAAEEGADYLGVGHIFPTSSKEKPMPPIGTSAIRPIIEASGLPVIAIG
GIELQNVAEVIKAGASGVAVISAVSGSADPVAATRELVKRIRQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=32.972972972973, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325042, Length=191, Percent_Identity=36.6492146596859, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CHLP8 (B3QNM2)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998725.1
- ProteinModelPortal:   B3QNM2
- GeneID:   6420049
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1118
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   CLSK637633
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21448; Mature: 21317

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPRRLLCVITDQQSNPVELARMALEGGARMVQLRRKSASGRDLFEWAVRIQALCREYE
CCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
ALFIVNDRVDIALAAHADGVHLGQQDIPVASARALLGPETLIGVSVSTPSEAAKAAEEGA
EEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCC
DYLGVGHIFPTSSKEKPMPPIGTSAIRPIIEASGLPVIAIGGIELQNVAEVIKAGASGVA
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEE
VISAVSGSADPVAATRELVKRIRQ
EEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPRRLLCVITDQQSNPVELARMALEGGARMVQLRRKSASGRDLFEWAVRIQALCREYE
CCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
ALFIVNDRVDIALAAHADGVHLGQQDIPVASARALLGPETLIGVSVSTPSEAAKAAEEGA
EEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCC
DYLGVGHIFPTSSKEKPMPPIGTSAIRPIIEASGLPVIAIGGIELQNVAEVIKAGASGVA
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEE
VISAVSGSADPVAATRELVKRIRQ
EEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA