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The map label for this gene is thiE [C]

Identifier: 193212771

GI number: 193212771

Start: 1235184

End: 1235822

Strand: Direct

Name: thiE [C]

Synonym: Cpar_1117

Alternate gene names: 193212771

Gene position: 1235184-1235822 (Clockwise)

Preceding gene: 193212766

Following gene: 193212772

Centisome position: 53.96

GC content: 57.9

Gene sequence:

>639_bases
ATGGTCAGAAAACAAACCCCACTCCCCCGCCTCATGATTGTCAGCAGTGGCGAGGAGCATCGTTCGCTGCAAGGACTTGC
GCTTCGCCAAGCCGAAGCTCTCGGCTGTTCAGCACCAGTGGTGTATCAGTTGCGAGAAAAAGGGCTCGATGCGGGAGAAC
TTGAAGCCCTCTGCCGACAGATTGTCCCTGCCATTAAGGCAACAGGATCACTTTTCACAGTCAACGAACGATTTGACATT
GCCCTGACTTCGAAAGCCTCGGGCGTCCACCTTCCCGAAGCCTCATGTCCTCCAGACGCGGTAAGAAAGGCTGCGCCCTC
GCTGATTGTCGGCCAGAGCGTGCATTCGGCAAAAGCGGCACGAGCTGCCACCGCGGCCGGACTTGATTATCTGCTTTTCG
GGCCGGTCTTTCAGACGCCATCCAAGGAACCGTTCGGCGCCCCACAAGGGCTCGAACGACTGCGCGAGGTCTGCCGGGCA
ACACCCCTGCCGGTCTTCGCGGTGGGAGGCATAACGCCAGAGCGCTCTCCGTCGTGCATTGAATGCGGAGCTTGGGGAGT
TGCCGCCATGAGACCCTTTTTTGATCCAGAAACCATGCCTGAAACGATCAACCTCTTCCTTTCGTATCTGCCCTCATGA

Upstream 100 bases:

>100_bases
TTTTTGATTTAAAAAACCGCCTATCCGTCACTCATATTGAGCGGCTCCAGGCAATTATGATTACTTTTGAAAAATAGTTA
TCGTGCCTGAAATTGCTTTC

Downstream 100 bases:

>100_bases
CTATTCCACGACGACTGCTTTGCGTCATCACCGACCAGCAATCCAACCCTGTCGAACTGGCCCGCATGGCCCTCGAAGGA
GGCGCGCGCATGGTACAACT

Product: thiamine monophosphate synthase

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVRKQTPLPRLMIVSSGEEHRSLQGLALRQAEALGCSAPVVYQLREKGLDAGELEALCRQIVPAIKATGSLFTVNERFDI
ALTSKASGVHLPEASCPPDAVRKAAPSLIVGQSVHSAKAARAATAAGLDYLLFGPVFQTPSKEPFGAPQGLERLREVCRA
TPLPVFAVGGITPERSPSCIECGAWGVAAMRPFFDPETMPETINLFLSYLPS

Sequences:

>Translated_212_residues
MVRKQTPLPRLMIVSSGEEHRSLQGLALRQAEALGCSAPVVYQLREKGLDAGELEALCRQIVPAIKATGSLFTVNERFDI
ALTSKASGVHLPEASCPPDAVRKAAPSLIVGQSVHSAKAARAATAAGLDYLLFGPVFQTPSKEPFGAPQGLERLREVCRA
TPLPVFAVGGITPERSPSCIECGAWGVAAMRPFFDPETMPETINLFLSYLPS
>Mature_212_residues
MVRKQTPLPRLMIVSSGEEHRSLQGLALRQAEALGCSAPVVYQLREKGLDAGELEALCRQIVPAIKATGSLFTVNERFDI
ALTSKASGVHLPEASCPPDAVRKAAPSLIVGQSVHSAKAARAATAAGLDYLLFGPVFQTPSKEPFGAPQGLERLREVCRA
TPLPVFAVGGITPERSPSCIECGAWGVAAMRPFFDPETMPETINLFLSYLPS

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=156, Percent_Identity=35.2564102564103, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 22596; Mature: 22596

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKQTPLPRLMIVSSGEEHRSLQGLALRQAEALGCSAPVVYQLREKGLDAGELEALCRQ
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
IVPAIKATGSLFTVNERFDIALTSKASGVHLPEASCPPDAVRKAAPSLIVGQSVHSAKAA
HHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHH
RAATAAGLDYLLFGPVFQTPSKEPFGAPQGLERLREVCRATPLPVFAVGGITPERSPSCI
HHHHHHCCHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEE
ECGAWGVAAMRPFFDPETMPETINLFLSYLPS
ECCCCCHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVRKQTPLPRLMIVSSGEEHRSLQGLALRQAEALGCSAPVVYQLREKGLDAGELEALCRQ
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
IVPAIKATGSLFTVNERFDIALTSKASGVHLPEASCPPDAVRKAAPSLIVGQSVHSAKAA
HHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHH
RAATAAGLDYLLFGPVFQTPSKEPFGAPQGLERLREVCRATPLPVFAVGGITPERSPSCI
HHHHHHCCHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEE
ECGAWGVAAMRPFFDPETMPETINLFLSYLPS
ECCCCCHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {hydroxymethylpyrimidine} 0.0004 {thiazole} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]