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The map label for this gene is hsdM [H]

Identifier: 193212615

GI number: 193212615

Start: 1061867

End: 1063333

Strand: Direct

Name: hsdM [H]

Synonym: Cpar_0960

Alternate gene names: 193212615

Gene position: 1061867-1063333 (Clockwise)

Preceding gene: 193212614

Following gene: 193212616

Centisome position: 46.38

GC content: 56.78

Gene sequence:

>1467_bases
ATGTCTATCCAGAACCTCGTAAAATCCATACAGGATATCATGCGCAAGGATGTTGGCGTTGATGGCGACGCTCAGCGCAT
CAGTCAGATTGTGTGGCTCCTTTTCCTGAAAATCTTTGATGACCGCGAGCAGGAGTGGCTGTTCACTCTGCCCGAATACC
GTTCGCCACTGCTGCCTCATCTTCGCTGGAGCAACTGGGCCAAAGACCCGGAAGGTATCACCGGTGAGGAGCTGATCGAT
TTCGTCAACAACGATCTTTTCCCCTCGCTGAAAAATCTCGCCACCACGCCTGGCGTTTCCTCGCATGGGCGCGTGGTTGG
CTCGGTGTTCGAGGATGCCTATAACTATATGAAGTCCGGTACGTTGCTGCGCCAGGTGATTAACATCATCGAGGAGGATG
TGGACTTCAATACCTCTGGCGACCGGCATCTGTTCAACGACATTTACGAAAAGCTGCTCGCCGATCTGCAATCCGCCGGA
AATGCTGGTGAATACTTCACGCCACGTGCGGTGACGCAGTTCATGGTGGACATGCTCGACCCGCAACTCGGCGAGTCTTT
GCTTGATCCGGCCTGCGGTACCGGCGGCTTTCTCACCTGCGCCATCGAGCATCTGAACGAGCAGGTCAAGACCGTCGATG
ACCGCGAAAAGATTCAGGAGAGCCTGCACGGCGTAGAGAAGAAGCCCCTGCCGCACATGCTGGCCGTGACGAACATGATG
CTGCATGGCATCGACGTACCGACTAACATCCGGCACGATAATACGCTCAGCCGTCCGCTCAAGGATTACAGCCCGAAAGA
TCGGGTCGATCTCATCATCACCAATCCGCCGTTCGGGGGGATGGAAGAGGATGGCATCGAGAAGAACTTTCCCCGCCAGT
ACCAGACCCGCGAAACCGCCGACCTGTTTATGGCGCTTATCATGCACCTGTTGAAGCCCGATACCGGCAAGGCTGCCGTG
GTGCTGCCGGACGGGTTCCTCTTTGGCGAAGGGGTCAAGACGACGCTCAAAAAAGAGCTGCTCGAATCGTTCGATCTGCA
CACCATTGTGCGGTTGCCCAAAGGGGTGTTCAGCCCCTACACCAGCATTGCGACCAACATTCTGTTTTTCAAGAAGGGCG
GGCCGACAAAAGAGGTCTGGTTCTTCGAGCATCCCTATCCGGAAGGGTACAAATCCTACTCCCGCTCGAAACCGCTGACT
ATCGCGGAGTTCGACCGAGAAAAGGCATGGTGGGGCGGAGCGTCGCGCAAAGGTCGCCAAACGACGGAGTACGCCTGGAA
GGTGAGCGCGGAGGAGATTGTGGCGCGCAACGGCAACCTCGATTGCAAGAATCCGCACGAGGTCGAGGTGGTGCATGGTG
ATCCGGAGGAGTTGATGCAGGAGTATCATGGGATCGTCCGCCAGCTCGAAGAGGCGCAAGAGGCGCTGAAGCGCGAGTTG
ATCGATGCGCTGGAGGGGAGGGGATGA

Upstream 100 bases:

>100_bases
GCGCTTTTTCGGCGGAGTGGATGGGTACCGCCAAGCGCTGCTCGATCTTGAGCATGAACTCTATTGCGCCTGAAAACAAC
AACTTGACAGACAATCAGTA

Downstream 100 bases:

>100_bases
CGATGGATTTTCGGGACGGCGAAACATCGGTGGTTGAGCTGCTTGAGCGGCATTTCGAGGTGGCGTTTTCTGCGCCGGAT
GGGGTGAAGAAGCTGCGGGA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSIQNLVKSIQDIMRKDVGVDGDAQRISQIVWLLFLKIFDDREQEWLFTLPEYRSPLLPHLRWSNWAKDPEGITGEELID
FVNNDLFPSLKNLATTPGVSSHGRVVGSVFEDAYNYMKSGTLLRQVINIIEEDVDFNTSGDRHLFNDIYEKLLADLQSAG
NAGEYFTPRAVTQFMVDMLDPQLGESLLDPACGTGGFLTCAIEHLNEQVKTVDDREKIQESLHGVEKKPLPHMLAVTNMM
LHGIDVPTNIRHDNTLSRPLKDYSPKDRVDLIITNPPFGGMEEDGIEKNFPRQYQTRETADLFMALIMHLLKPDTGKAAV
VLPDGFLFGEGVKTTLKKELLESFDLHTIVRLPKGVFSPYTSIATNILFFKKGGPTKEVWFFEHPYPEGYKSYSRSKPLT
IAEFDREKAWWGGASRKGRQTTEYAWKVSAEEIVARNGNLDCKNPHEVEVVHGDPEELMQEYHGIVRQLEEAQEALKREL
IDALEGRG

Sequences:

>Translated_488_residues
MSIQNLVKSIQDIMRKDVGVDGDAQRISQIVWLLFLKIFDDREQEWLFTLPEYRSPLLPHLRWSNWAKDPEGITGEELID
FVNNDLFPSLKNLATTPGVSSHGRVVGSVFEDAYNYMKSGTLLRQVINIIEEDVDFNTSGDRHLFNDIYEKLLADLQSAG
NAGEYFTPRAVTQFMVDMLDPQLGESLLDPACGTGGFLTCAIEHLNEQVKTVDDREKIQESLHGVEKKPLPHMLAVTNMM
LHGIDVPTNIRHDNTLSRPLKDYSPKDRVDLIITNPPFGGMEEDGIEKNFPRQYQTRETADLFMALIMHLLKPDTGKAAV
VLPDGFLFGEGVKTTLKKELLESFDLHTIVRLPKGVFSPYTSIATNILFFKKGGPTKEVWFFEHPYPEGYKSYSRSKPLT
IAEFDREKAWWGGASRKGRQTTEYAWKVSAEEIVARNGNLDCKNPHEVEVVHGDPEELMQEYHGIVRQLEEAQEALKREL
IDALEGRG
>Mature_487_residues
SIQNLVKSIQDIMRKDVGVDGDAQRISQIVWLLFLKIFDDREQEWLFTLPEYRSPLLPHLRWSNWAKDPEGITGEELIDF
VNNDLFPSLKNLATTPGVSSHGRVVGSVFEDAYNYMKSGTLLRQVINIIEEDVDFNTSGDRHLFNDIYEKLLADLQSAGN
AGEYFTPRAVTQFMVDMLDPQLGESLLDPACGTGGFLTCAIEHLNEQVKTVDDREKIQESLHGVEKKPLPHMLAVTNMML
HGIDVPTNIRHDNTLSRPLKDYSPKDRVDLIITNPPFGGMEEDGIEKNFPRQYQTRETADLFMALIMHLLKPDTGKAAVV
LPDGFLFGEGVKTTLKKELLESFDLHTIVRLPKGVFSPYTSIATNILFFKKGGPTKEVWFFEHPYPEGYKSYSRSKPLTI
AEFDREKAWWGGASRKGRQTTEYAWKVSAEEIVARNGNLDCKNPHEVEVVHGDPEELMQEYHGIVRQLEEAQEALKRELI
DALEGRG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=432, Percent_Identity=28.9351851851852, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55381; Mature: 55249

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCC
>Mature Secondary Structure 
SIQNLVKSIQDIMRKDVGVDGDAQRISQIVWLLFLKIFDDREQEWLFTLPEYRSPLLPH
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEECHHHCCCCCCC
LRWSNWAKDPEGITGEELIDFVNNDLFPSLKNLATTPGVSSHGRVVGSVFEDAYNYMKSG
CCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TLLRQVINIIEEDVDFNTSGDRHLFNDIYEKLLADLQSAGNAGEYFTPRAVTQFMVDMLD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLGESLLDPACGTGGFLTCAIEHLNEQVKTVDDREKIQESLHGVEKKPLPHMLAVTNMM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LHGIDVPTNIRHDNTLSRPLKDYSPKDRVDLIITNPPFGGMEEDGIEKNFPRQYQTRETA
HHCCCCCCCCCCCCHHHCCHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
DLFMALIMHLLKPDTGKAAVVLPDGFLFGEGVKTTLKKELLESFDLHTIVRLPKGVFSPY
HHHHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHCHHHHHHCCHHHHCHH
TSIATNILFFKKGGPTKEVWFFEHPYPEGYKSYSRSKPLTIAEFDREKAWWGGASRKGRQ
HHHHHHEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCC
TTEYAWKVSAEEIVARNGNLDCKNPHEVEVVHGDPEELMQEYHGIVRQLEEAQEALKREL
CHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDALEGRG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]