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The map label for this gene is hsdS [H]

Identifier: 193212616

GI number: 193212616

Start: 1063330

End: 1065066

Strand: Direct

Name: hsdS [H]

Synonym: Cpar_0961

Alternate gene names: 193212616

Gene position: 1063330-1065066 (Clockwise)

Preceding gene: 193212615

Following gene: 193212617

Centisome position: 46.45

GC content: 46.34

Gene sequence:

>1737_bases
ATGACGATGGATTTTCGGGACGGCGAAACATCGGTGGTTGAGCTGCTTGAGCGGCATTTCGAGGTGGCGTTTTCTGCGCC
GGATGGGGTGAAGAAGCTGCGGGAGTTGATTCTGACGCTTGCGATGAAGGGCAAACTCGTGCCGCAGGATCCGAACGATC
AACCGGCGAGTGAGATGCTGAAGGAGATTGAAGCTGAAAAACAGCGGTTGGTGAAAGAGGGGAAAATCAAGAATCCGAAA
GCGTTACCGCCGATTACGGCTGACGATGTTCCGTATGAATTGCCGGATGGTTGGGAGTGGGTAAGGCTTGGTGAAATTAC
AGCCTATAACGGTAGAAAAAATATATCAGGAGATCAAATTGATCCAGATACCTGGGTGCTTGATCTTGAGGATATTGAAA
AGGATACTTCAAGAATTTTATATCGCGCGAAGTTCTCTGAAAGACAATCAAAATCAACAAAATCTACTTTTTTAAAAGGT
GATGTGCTCTACGGTAAACTACGTCCATATTTGGATAAAATAGTTGTTGCTGATAGAGATGGGGTTTGTACTACTGAAAT
TGTTCCTATTGTTTCTTTCGTTGGATTGCATTCTGATTTTCTCAAATGGTTATTAAAACGTCCAGCATTTTTATCCTATG
TCAATTCTTTGATGTATGGTGTGAAAATGCCTCGACTTGGAACGGATAATGCAGTGGCGAGTATTCATCCACTTCCACCA
CTTCCCGAACAACACCGTATCGTCGCTCGTATCGATGAATTGATGGCGCATTGTGATGAGCTGGAAAAGCTGCGTGCGGA
GCGGGAGCAGAAGCGCGTCAAAGTTCACGCCGCCGCAGTCAGGCAGTTGCTCGATACGACGGAGCCGGAGTCAAGCGCCA
ACGCATGGCAGTTCATCTCCCGGAATTTCCGTGAACTTTATAGCGATAAGGAAAACGTCGCCGAACTCCGCAAAGCCATT
CTCCAGCTCGCCGTCATGGGCAAGCTCGTCCCGCAAGACCCGAATGATCCGCCTGCCTGTGAACTGTTGAAGGAGATTGA
AGCTGAGAAACAGCGGTTGGTCAAAGAGGGGAAGATCAAAAAGCCGAAGGCGGTGTCGCCGATTAAGCCGGATGAAGTTC
CGTATCCGCTGCCGGATAGTTGGGAGTGGGTGAGATTGGGAGACGTGATTTCTTATATGGATGCAGGTTGGAGTCCGAAG
TGCGAAACTGGACCGGCAAGTGATTCGGAATGGGGAGTTTTAAAAACCACAGCGGTTCAAAAATTGGAATTCTTGCCGCA
TGAAAATAAGACTTTGCCTATAAAATTAACACCTCGACCAGAATATCAGGTAGAGGAAAAAGATATTTTGATTACTCGTG
CAGGCCCAAAGAATCGAGTTGGTATATGTTGTGTTGCTACGTCTATAAGGCCTAAACTGATGTTATCGGATAAAATTATT
CGGTTCAAAATCTATGGTGATCTGATTTCCCCGGATTATTGCGCTCTTTCTTTGAATACAGGATATTGTTCCGAACAAAT
TGAGATGTTTAAATCTGGTATGGCTGAGAGTCAAATGAATATTTCTCAAGATAAGGTGAAACGTTTGTTGATGCTAATTC
CCCCACTTCCCGAACAACACCGCATCGTCGCTCGCATCGATCAGTTGATGGCTTTGTGCGATACGCTGGAGCAGCAGATC
GATGACGCCACCCGTAAACAAACCGAACTACTCAACGCCGTGATGACTCAGGTGTAG

Upstream 100 bases:

>100_bases
CGGAGGAGTTGATGCAGGAGTATCATGGGATCGTCCGCCAGCTCGAAGAGGCGCAAGAGGCGCTGAAGCGCGAGTTGATC
GATGCGCTGGAGGGGAGGGG

Downstream 100 bases:

>100_bases
GAGGGGAAAACTATGCGCCTGACATCACTCTACATCGGCCAATACAAAAATCTGCGGGATTTTTCCCTGAGCTTCGATGG
CAGCAGTTTCATCGATGTCT

Product: restriction modification system DNA specificity domain

Products: NA

Alternate protein names: S.EcoEI; Type I restriction enzyme EcoEI specificity protein; S protein [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MTMDFRDGETSVVELLERHFEVAFSAPDGVKKLRELILTLAMKGKLVPQDPNDQPASEMLKEIEAEKQRLVKEGKIKNPK
ALPPITADDVPYELPDGWEWVRLGEITAYNGRKNISGDQIDPDTWVLDLEDIEKDTSRILYRAKFSERQSKSTKSTFLKG
DVLYGKLRPYLDKIVVADRDGVCTTEIVPIVSFVGLHSDFLKWLLKRPAFLSYVNSLMYGVKMPRLGTDNAVASIHPLPP
LPEQHRIVARIDELMAHCDELEKLRAEREQKRVKVHAAAVRQLLDTTEPESSANAWQFISRNFRELYSDKENVAELRKAI
LQLAVMGKLVPQDPNDPPACELLKEIEAEKQRLVKEGKIKKPKAVSPIKPDEVPYPLPDSWEWVRLGDVISYMDAGWSPK
CETGPASDSEWGVLKTTAVQKLEFLPHENKTLPIKLTPRPEYQVEEKDILITRAGPKNRVGICCVATSIRPKLMLSDKII
RFKIYGDLISPDYCALSLNTGYCSEQIEMFKSGMAESQMNISQDKVKRLLMLIPPLPEQHRIVARIDQLMALCDTLEQQI
DDATRKQTELLNAVMTQV

Sequences:

>Translated_578_residues
MTMDFRDGETSVVELLERHFEVAFSAPDGVKKLRELILTLAMKGKLVPQDPNDQPASEMLKEIEAEKQRLVKEGKIKNPK
ALPPITADDVPYELPDGWEWVRLGEITAYNGRKNISGDQIDPDTWVLDLEDIEKDTSRILYRAKFSERQSKSTKSTFLKG
DVLYGKLRPYLDKIVVADRDGVCTTEIVPIVSFVGLHSDFLKWLLKRPAFLSYVNSLMYGVKMPRLGTDNAVASIHPLPP
LPEQHRIVARIDELMAHCDELEKLRAEREQKRVKVHAAAVRQLLDTTEPESSANAWQFISRNFRELYSDKENVAELRKAI
LQLAVMGKLVPQDPNDPPACELLKEIEAEKQRLVKEGKIKKPKAVSPIKPDEVPYPLPDSWEWVRLGDVISYMDAGWSPK
CETGPASDSEWGVLKTTAVQKLEFLPHENKTLPIKLTPRPEYQVEEKDILITRAGPKNRVGICCVATSIRPKLMLSDKII
RFKIYGDLISPDYCALSLNTGYCSEQIEMFKSGMAESQMNISQDKVKRLLMLIPPLPEQHRIVARIDQLMALCDTLEQQI
DDATRKQTELLNAVMTQV
>Mature_577_residues
TMDFRDGETSVVELLERHFEVAFSAPDGVKKLRELILTLAMKGKLVPQDPNDQPASEMLKEIEAEKQRLVKEGKIKNPKA
LPPITADDVPYELPDGWEWVRLGEITAYNGRKNISGDQIDPDTWVLDLEDIEKDTSRILYRAKFSERQSKSTKSTFLKGD
VLYGKLRPYLDKIVVADRDGVCTTEIVPIVSFVGLHSDFLKWLLKRPAFLSYVNSLMYGVKMPRLGTDNAVASIHPLPPL
PEQHRIVARIDELMAHCDELEKLRAEREQKRVKVHAAAVRQLLDTTEPESSANAWQFISRNFRELYSDKENVAELRKAIL
QLAVMGKLVPQDPNDPPACELLKEIEAEKQRLVKEGKIKKPKAVSPIKPDEVPYPLPDSWEWVRLGDVISYMDAGWSPKC
ETGPASDSEWGVLKTTAVQKLEFLPHENKTLPIKLTPRPEYQVEEKDILITRAGPKNRVGICCVATSIRPKLMLSDKIIR
FKIYGDLISPDYCALSLNTGYCSEQIEMFKSGMAESQMNISQDKVKRLLMLIPPLPEQHRIVARIDQLMALCDTLEQQID
DATRKQTELLNAVMTQV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 65604; Mature: 65472

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDFRDGETSVVELLERHFEVAFSAPDGVKKLRELILTLAMKGKLVPQDPNDQPASEML
CCCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KEIEAEKQRLVKEGKIKNPKALPPITADDVPYELPDGWEWVRLGEITAYNGRKNISGDQI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCC
DPDTWVLDLEDIEKDTSRILYRAKFSERQSKSTKSTFLKGDVLYGKLRPYLDKIVVADRD
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCC
GVCTTEIVPIVSFVGLHSDFLKWLLKRPAFLSYVNSLMYGVKMPRLGTDNAVASIHPLPP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
LPEQHRIVARIDELMAHCDELEKLRAEREQKRVKVHAAAVRQLLDTTEPESSANAWQFIS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RNFRELYSDKENVAELRKAILQLAVMGKLVPQDPNDPPACELLKEIEAEKQRLVKEGKIK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KPKAVSPIKPDEVPYPLPDSWEWVRLGDVISYMDAGWSPKCETGPASDSEWGVLKTTAVQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
KLEFLPHENKTLPIKLTPRPEYQVEEKDILITRAGPKNRVGICCVATSIRPKLMLSDKII
HHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCEEECCCEE
RFKIYGDLISPDYCALSLNTGYCSEQIEMFKSGMAESQMNISQDKVKRLLMLIPPLPEQH
EEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCCHH
RIVARIDQLMALCDTLEQQIDDATRKQTELLNAVMTQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMDFRDGETSVVELLERHFEVAFSAPDGVKKLRELILTLAMKGKLVPQDPNDQPASEML
CCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KEIEAEKQRLVKEGKIKNPKALPPITADDVPYELPDGWEWVRLGEITAYNGRKNISGDQI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCC
DPDTWVLDLEDIEKDTSRILYRAKFSERQSKSTKSTFLKGDVLYGKLRPYLDKIVVADRD
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCC
GVCTTEIVPIVSFVGLHSDFLKWLLKRPAFLSYVNSLMYGVKMPRLGTDNAVASIHPLPP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
LPEQHRIVARIDELMAHCDELEKLRAEREQKRVKVHAAAVRQLLDTTEPESSANAWQFIS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RNFRELYSDKENVAELRKAILQLAVMGKLVPQDPNDPPACELLKEIEAEKQRLVKEGKIK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KPKAVSPIKPDEVPYPLPDSWEWVRLGDVISYMDAGWSPKCETGPASDSEWGVLKTTAVQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
KLEFLPHENKTLPIKLTPRPEYQVEEKDILITRAGPKNRVGICCVATSIRPKLMLSDKII
HHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCEEECCCEE
RFKIYGDLISPDYCALSLNTGYCSEQIEMFKSGMAESQMNISQDKVKRLLMLIPPLPEQH
EEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCCHH
RIVARIDQLMALCDTLEQQIDDATRKQTELLNAVMTQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]