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The map label for this gene is dnaN [H]

Identifier: 193211678

GI number: 193211678

Start: 1934

End: 3058

Strand: Direct

Name: dnaN [H]

Synonym: Cpar_0002

Alternate gene names: 193211678

Gene position: 1934-3058 (Clockwise)

Preceding gene: 193211677

Following gene: 193211679

Centisome position: 0.08

GC content: 51.29

Gene sequence:

>1125_bases
ATGAAATTCAACACGACAATCAAACGCCTGCAGGAAGCGGTGAGTAAAGTCATCCTTGCTGTGCCTACCAAATCACTCGA
TGCCCGGTTCGATAACATCAACATGACCCTGGAGAACGGGACACTGACGATGTTTGCCACCGATGGAGAGCTTTCGATTA
CCGCCAAGTGTGATGTGGCCTCCAGCGACAAGGGAACCATCGCCATCAGGGCAAGAACCTTGCAGGATTTTCTGCGCAGC
ATGTACGATACCGAAGTCACCTTCGAAATCGAGCGCCAGTCGATCGGAGATCTTGGCACGGTTCATATCGCTACCGACAA
GGGCCGGTATCGTATTCCCTGCACCTTCATCTCGAAACCTGAAAGTCTCGAACGGAATTTCGACCTCACCATCGGACTTC
AACCTTCCGAATTGCAGGATATCATCCAGAAAACCCTGTTTGCCTGCAGCGTCGATGGCATGCGCCCGGCCATGATGGGG
GTGCTGTTTGAATTCGAGGGAACGGTCATTACCGCTGTTGCAACCGATGGCCATCGTCTGGTCCGGTGCCGCAAGCAAAC
CACAAACGAAATTGCCGAAAAGCAGAAAATCGTAGTCCCTGCACGGGTTCTTTCCATTATCCAGCGTCTTTTGCAGAACG
ATGAGGTCAGCATGACCGTCGATGAAGAGCGCCGGAATGTGAAGTTTAAATCGGAAAATGTCGAGCTTGAATCGGCTTTG
ATTGTCGAGCCATATCCCAATTATGAAGCGGTTATTCCGGTTGAAAACGAGAAAAAACTGGTGGTTGATCGCGGTTTGAT
TCACGATTCGGTCAAAAGGGTTGGCCGTTTCTCAAGTATCGGTGATCTGAAGATCAGCGCCGAAGGAGAGCTCATGAAGG
TCATGGCCGAAAATGCCAGCGAGGGTGAATCCGCACAGGAAGAGCTGCCGTGTACGTTCACCGGTGAAGAGATCACGATC
GGTTTTAATGCAAAGTTTGTCGAAGCCGCCCTGTCCCATATCGAAACCGAGCAGGTCTCCATGGAAATGAGCAGCCCGAC
AACGGCCGTGTTGTTCAAACCGAAATACGAGGAGCGCCAGGACGATCTGATCATTCTGGTGATGCCGGTCAGAATCAACA
ATTGA

Upstream 100 bases:

>100_bases
AAGAAGATAAAAGCAGTCTTCGTGGATCAGTCAAGAATTCCACACGCTCAACAACAACAACAATCTTTTTATTTAAATAA
AGAAATAAGAGTTGTAAGAA

Downstream 100 bases:

>100_bases
CGGAACAGGAGAGGCTTGCGACTCGACTCCATCAGTATCGCCAATTTCAGGAATCACACGCTTCTTGAATTCGAGCCAGG
CCACTCCGTTACCAATATTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFNTTIKRLQEAVSKVILAVPTKSLDARFDNINMTLENGTLTMFATDGELSITAKCDVASSDKGTIAIRARTLQDFLRS
MYDTEVTFEIERQSIGDLGTVHIATDKGRYRIPCTFISKPESLERNFDLTIGLQPSELQDIIQKTLFACSVDGMRPAMMG
VLFEFEGTVITAVATDGHRLVRCRKQTTNEIAEKQKIVVPARVLSIIQRLLQNDEVSMTVDEERRNVKFKSENVELESAL
IVEPYPNYEAVIPVENEKKLVVDRGLIHDSVKRVGRFSSIGDLKISAEGELMKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSMEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN

Sequences:

>Translated_374_residues
MKFNTTIKRLQEAVSKVILAVPTKSLDARFDNINMTLENGTLTMFATDGELSITAKCDVASSDKGTIAIRARTLQDFLRS
MYDTEVTFEIERQSIGDLGTVHIATDKGRYRIPCTFISKPESLERNFDLTIGLQPSELQDIIQKTLFACSVDGMRPAMMG
VLFEFEGTVITAVATDGHRLVRCRKQTTNEIAEKQKIVVPARVLSIIQRLLQNDEVSMTVDEERRNVKFKSENVELESAL
IVEPYPNYEAVIPVENEKKLVVDRGLIHDSVKRVGRFSSIGDLKISAEGELMKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSMEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN
>Mature_374_residues
MKFNTTIKRLQEAVSKVILAVPTKSLDARFDNINMTLENGTLTMFATDGELSITAKCDVASSDKGTIAIRARTLQDFLRS
MYDTEVTFEIERQSIGDLGTVHIATDKGRYRIPCTFISKPESLERNFDLTIGLQPSELQDIIQKTLFACSVDGMRPAMMG
VLFEFEGTVITAVATDGHRLVRCRKQTTNEIAEKQKIVVPARVLSIIQRLLQNDEVSMTVDEERRNVKFKSENVELESAL
IVEPYPNYEAVIPVENEKKLVVDRGLIHDSVKRVGRFSSIGDLKISAEGELMKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSMEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=379, Percent_Identity=22.9551451187335, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41839; Mature: 41839

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNTTIKRLQEAVSKVILAVPTKSLDARFDNINMTLENGTLTMFATDGELSITAKCDVA
CCCCHHHHHHHHHHHHEEEEECCCHHCCEECCEEEEEECCEEEEEEECCCEEEEEEEECC
SSDKGTIAIRARTLQDFLRSMYDTEVTFEIERQSIGDLGTVHIATDKGRYRIPCTFISKP
CCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCC
ESLERNFDLTIGLQPSELQDIIQKTLFACSVDGMRPAMMGVLFEFEGTVITAVATDGHRL
CHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCEEEEEEECCCHHH
VRCRKQTTNEIAEKQKIVVPARVLSIIQRLLQNDEVSMTVDEERRNVKFKSENVELESAL
HHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCEEEEECCHHCCCEEECCCEEEEEEE
IVEPYPNYEAVIPVENEKKLVVDRGLIHDSVKRVGRFSSIGDLKISAEGELMKVMAENAS
EECCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCEEEECCCCEEHHHHCCCC
EGESAQEELPCTFTGEEITIGFNAKFVEAALSHIETEQVSMEMSSPTTAVLFKPKYEERQ
CCCCHHHHCCEEECCCEEEEECCCHHHHHHHHHCCHHHEEEEECCCCEEEEECCCCCCCC
DDLIILVMPVRINN
CCEEEEEEEEEECC
>Mature Secondary Structure
MKFNTTIKRLQEAVSKVILAVPTKSLDARFDNINMTLENGTLTMFATDGELSITAKCDVA
CCCCHHHHHHHHHHHHEEEEECCCHHCCEECCEEEEEECCEEEEEEECCCEEEEEEEECC
SSDKGTIAIRARTLQDFLRSMYDTEVTFEIERQSIGDLGTVHIATDKGRYRIPCTFISKP
CCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCC
ESLERNFDLTIGLQPSELQDIIQKTLFACSVDGMRPAMMGVLFEFEGTVITAVATDGHRL
CHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCEEEEEEECCCHHH
VRCRKQTTNEIAEKQKIVVPARVLSIIQRLLQNDEVSMTVDEERRNVKFKSENVELESAL
HHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCEEEEECCHHCCCEEECCCEEEEEEE
IVEPYPNYEAVIPVENEKKLVVDRGLIHDSVKRVGRFSSIGDLKISAEGELMKVMAENAS
EECCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCEEEECCCCEEHHHHCCCC
EGESAQEELPCTFTGEEITIGFNAKFVEAALSHIETEQVSMEMSSPTTAVLFKPKYEERQ
CCCCHHHHCCEEECCCEEEEECCCHHHHHHHHHCCHHHEEEEECCCCEEEEECCCCCCCC
DDLIILVMPVRINN
CCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]