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The map label for this gene is recF

Identifier: 193211679

GI number: 193211679

Start: 3074

End: 4180

Strand: Direct

Name: recF

Synonym: Cpar_0003

Alternate gene names: 193211679

Gene position: 3074-4180 (Clockwise)

Preceding gene: 193211678

Following gene: 193211680

Centisome position: 0.13

GC content: 45.8

Gene sequence:

>1107_bases
TTGCGACTCGACTCCATCAGTATCGCCAATTTCAGGAATCACACGCTTCTTGAATTCGAGCCAGGCCACTCCGTTACCAA
TATTTATGGCAGAAATGGTTCGGGAAAAACCTCGATTCTTGAGGCGATCCATTACTGCGCTCTGACAAGAGGCTTTTCTG
GAAATAACGATCGGGAGTATCTCAAGTTCGGGGAGGAGCTGTTTACCATCCGCTCCTCATTTACGAGTGGTCAGGGAATT
GCTACCAAAGTTTCGGTCGCCTATTCACCGAAGAGGGAGAAGAGAATCCTGGTTAATGAACAGGAGCTTCAAACCTTTTC
CAGCCATATCGGAACCATTCCGTGCGTGACCTTTACGCCACGTGAGATGGTAATCATCAACGGCGCACCGGCGGAGCGTC
GTCGATTCATCGATACGGCAATCTGTCAGTATGATCGGAAGTACTTATCCGATTTATTGCTGTATCGGCGTATTTTACAG
CAACGTAATGCCCTGCTTTCTTCGGAGCAGGATCCGCGTTTTATTGATTCAGCTCTCGATGTTCTAACCGATCAACTCGT
TGCTACAGCAACGGAAATTGTTCTGGTCAGAAAGCGATTTATCGAGCATTTTACTTCCATGCTTGGTGATGTCTATCAAT
GGATCCCCGAAGGAGCTGAGCCATCAATTTTATATCAAAGTTCTCTTGGCCATCACGAAAATCTGTATGAAAAAGATAAA
ATACAGCAGGTTTTCAGAGAGCGTTTTGAAACGTTAAAGCAGCAGGAGTTACAACGAAGACAAACGCTTGCTGGACCACA
TCGAGACGATTTGCAATTCTATCTCAATAAAAGAGAAATACGAAAATATGCCTCTCAGGGGCAACAGCGTGCATTTCTTG
TCGCTATGAAAATGACCCTGCAAGGCTATCTTTATGAAGCATCGGGCGAGATACCGATTACCCTTCTTGACGATCTGTTC
AGTGAGCTTGATGAGGTGGTTTCCGGTACCATGGTCGAGACGCTTGCAACCAAAGGACAGGTGATTATCACCTCAACGGG
GAAAAAAGAGGGGAAGGGGATAAGTTGTTTTTCTGTTGATGATTACAAGAGTAGTGAGGAGCCTTGA

Upstream 100 bases:

>100_bases
AACGGCCGTGTTGTTCAAACCGAAATACGAGGAGCGCCAGGACGATCTGATCATTCTGGTGATGCCGGTCAGAATCAACA
ATTGACGGAACAGGAGAGGC

Downstream 100 bases:

>100_bases
TCATGAGTAAAGCAAAACCACCGAGAGAACTTGGCGGGATCATGGCCGATGTTTGCCGCAAGATCGGCATGACGGAAGCC
TATGATGAATACAAGACACT

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRLDSISIANFRNHTLLEFEPGHSVTNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSVAYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRFIDSALDVLTDQLVATATEIVLVRKRFIEHFTSMLGDVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTGKKEGKGISCFSVDDYKSSEEP

Sequences:

>Translated_368_residues
MRLDSISIANFRNHTLLEFEPGHSVTNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSVAYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRFIDSALDVLTDQLVATATEIVLVRKRFIEHFTSMLGDVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTGKKEGKGISCFSVDDYKSSEEP
>Mature_368_residues
MRLDSISIANFRNHTLLEFEPGHSVTNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSVAYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRFIDSALDVLTDQLVATATEIVLVRKRFIEHFTSMLGDVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTGKKEGKGISCFSVDDYKSSEEP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=349, Percent_Identity=24.3553008595989, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CHLP8 (B3QQY5)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001997632.1
- GeneID:   6418902
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0003
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK2303985
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- TIGRFAMs:   TIGR00611

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42039; Mature: 42039

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDSISIANFRNHTLLEFEPGHSVTNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREY
CCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKFGEELFTIRSSFTSGQGIATKVSVAYSPKREKRILVNEQELQTFSSHIGTIPCVTFTP
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCEEEECC
REMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQQRNALLSSEQDPRFIDSALD
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLTDQLVATATEIVLVRKRFIEHFTSMLGDVYQWIPEGAEPSILYQSSLGHHENLYEKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHH
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QGYLYEASGEIPITLLDDLFSELDEVVSGTMVETLATKGQVIITSTGKKEGKGISCFSVD
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
DYKSSEEP
CCCCCCCC
>Mature Secondary Structure
MRLDSISIANFRNHTLLEFEPGHSVTNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREY
CCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKFGEELFTIRSSFTSGQGIATKVSVAYSPKREKRILVNEQELQTFSSHIGTIPCVTFTP
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCEEEECC
REMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQQRNALLSSEQDPRFIDSALD
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLTDQLVATATEIVLVRKRFIEHFTSMLGDVYQWIPEGAEPSILYQSSLGHHENLYEKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHH
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QGYLYEASGEIPITLLDDLFSELDEVVSGTMVETLATKGQVIITSTGKKEGKGISCFSVD
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
DYKSSEEP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA