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The map label for this gene is gatA [H]

Identifier: 192290293

GI number: 192290293

Start: 2045944

End: 2047416

Strand: Reverse

Name: gatA [H]

Synonym: Rpal_1900

Alternate gene names: 192290293

Gene position: 2047416-2045944 (Counterclockwise)

Preceding gene: 192290294

Following gene: 192290291

Centisome position: 35.64

GC content: 69.72

Gene sequence:

>1473_bases
TTGCCCGCCTCGCCCTGGAGCTTTGCCACCGTCACCGATCTTTCCGCCGCCCTGAAGGCCAAGCAGGTCTCCGCCGTCGA
ACTCACGCAGGACGCGATCGGACGGATCGAGCGGCACGACGACAAGGTCAACGCGGTGTGCGTCCGCGACTTCGACAGGG
CGCTGCAGAGCGCGCGCGCAGCCGACCTCGCGCTGTCGCGCGGCGGGCGCGAGCCGCTGCTCGGCATTCCGATGACCGTC
AAGGAGAGCTTCAACGTCGCCGGCCTGCCGACCACCTGGGGTTTCGTCGAGCAGAAGAACTTCGTCGCTGCCGCCGATGC
GCTGGCGGTCGAGCGGGTCAAGGCCGCGGGCGGCGTGATCCTTGGCAAAACCAACGTGCCGGTCGCACTCGGCGACTGGC
AAAGCTACAACGAGATCTACGGCACCACCGGCAATCCGTATGATCTCGGCCGCACGCCGGGCGGATCGTCCGGTGGCTCC
TCGGCCGCCCTGGCCGCAGGCTTCGGCGCGCTGTCGCTCGGCTCGGATATCGGCGGCTCGCTGCGGGTGCCGGCGCATTA
CTGCGGCGTCTACGCCCACAAGCCGACCTTCGGCCTGTGCCCGGCACGCGGCCACACGCCGCCGCCGTTCCCGCCGCTGC
CGTCCAATCGCGATCTGTCGGTGATCGGCCCGATGGCGCGCTCGGCCGCCGATCTGGCGCTGCTGCTCGACGTCATCGCC
GGCACCGATCCGATCTTCGACGGCATCGCCTATCAGCTCGATCTGCCGGCGGCACGGCACACCGCGCTGAAGGACTTCCG
CGTGCTGCTGCTGGATACGCATCCGCTGCTGCCGACCGGCGCGGCCGTACACAGCGCACTGCAGACGCTGGCGGACGGCC
TGACCAAGGCCGGCGCGAGCGTCACCCGCTCCTCGCCGCTGCTGCCGGATCTCGCCGAGACCTCGCGGGTCTATATGCGG
ATGCTGCTGTCGTTCCTCGCCGCCGGCTTCCCCGCCGATCTCTATGCCGGCGCGCAGGCGATGGCAGCCGGACTCAGTGC
CACCGACGACAGCCTCGCTGCGGAGCGGCTGCGCGGTATCGTGCTCAGCCACCGCGACTGGGTGGCCGCCGACAGCCGCC
GCACCGGTTTGCGCGCGCAGTGGCGCGAGCTGTTCAAATCGTTCGACGCGGTGATCTGCCCGGTGATGCCGACGCCTGCC
TATCCGCACGACCACCACGAGCCGCAGGAAGAGCGCCGCATCCTGATCGACGGCAAGCCGCATCCTTACGTCGATCAGTT
GGTGTGGCCCGGCATCGCCACCCTGCCCGGCCTGCCGTCGACCGCGCTGCCGATCGCGATGTCGGACGACGGCCTGCCGA
TCGGCGTGCAGATCGTCGGCCCGTGGCTGGAAGACCGCACGCCGCTCAAGCTCGCCGAGTTGATCGAGCGCGAATTCGGC
GGCTTCAAACCGCCGAAGGCACTCGTCGATTAG

Upstream 100 bases:

>100_bases
ACGGCATCTTGGAGGCGGTGCGGCAAGGCACTACCGCCCGCCCGAGAACCGATTAAGCTCCCGCGCGCTCATGCCCGCCT
CGAACTCGGAGTCTGCCATC

Downstream 100 bases:

>100_bases
TTAGCGGCGAACGTCACCACAATCCGTCGTCATTGCGAGCGAAGCGAAGCAATCCAGAGCCCCGGGCACAGCGCTGGATT
GCTTTGTCGCTTCGCTCTTC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MPASPWSFATVTDLSAALKAKQVSAVELTQDAIGRIERHDDKVNAVCVRDFDRALQSARAADLALSRGGREPLLGIPMTV
KESFNVAGLPTTWGFVEQKNFVAAADALAVERVKAAGGVILGKTNVPVALGDWQSYNEIYGTTGNPYDLGRTPGGSSGGS
SAALAAGFGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPFPPLPSNRDLSVIGPMARSAADLALLLDVIA
GTDPIFDGIAYQLDLPAARHTALKDFRVLLLDTHPLLPTGAAVHSALQTLADGLTKAGASVTRSSPLLPDLAETSRVYMR
MLLSFLAAGFPADLYAGAQAMAAGLSATDDSLAAERLRGIVLSHRDWVAADSRRTGLRAQWRELFKSFDAVICPVMPTPA
YPHDHHEPQEERRILIDGKPHPYVDQLVWPGIATLPGLPSTALPIAMSDDGLPIGVQIVGPWLEDRTPLKLAELIEREFG
GFKPPKALVD

Sequences:

>Translated_490_residues
MPASPWSFATVTDLSAALKAKQVSAVELTQDAIGRIERHDDKVNAVCVRDFDRALQSARAADLALSRGGREPLLGIPMTV
KESFNVAGLPTTWGFVEQKNFVAAADALAVERVKAAGGVILGKTNVPVALGDWQSYNEIYGTTGNPYDLGRTPGGSSGGS
SAALAAGFGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPFPPLPSNRDLSVIGPMARSAADLALLLDVIA
GTDPIFDGIAYQLDLPAARHTALKDFRVLLLDTHPLLPTGAAVHSALQTLADGLTKAGASVTRSSPLLPDLAETSRVYMR
MLLSFLAAGFPADLYAGAQAMAAGLSATDDSLAAERLRGIVLSHRDWVAADSRRTGLRAQWRELFKSFDAVICPVMPTPA
YPHDHHEPQEERRILIDGKPHPYVDQLVWPGIATLPGLPSTALPIAMSDDGLPIGVQIVGPWLEDRTPLKLAELIEREFG
GFKPPKALVD
>Mature_489_residues
PASPWSFATVTDLSAALKAKQVSAVELTQDAIGRIERHDDKVNAVCVRDFDRALQSARAADLALSRGGREPLLGIPMTVK
ESFNVAGLPTTWGFVEQKNFVAAADALAVERVKAAGGVILGKTNVPVALGDWQSYNEIYGTTGNPYDLGRTPGGSSGGSS
AALAAGFGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPFPPLPSNRDLSVIGPMARSAADLALLLDVIAG
TDPIFDGIAYQLDLPAARHTALKDFRVLLLDTHPLLPTGAAVHSALQTLADGLTKAGASVTRSSPLLPDLAETSRVYMRM
LLSFLAAGFPADLYAGAQAMAAGLSATDDSLAAERLRGIVLSHRDWVAADSRRTGLRAQWRELFKSFDAVICPVMPTPAY
PHDHHEPQEERRILIDGKPHPYVDQLVWPGIATLPGLPSTALPIAMSDDGLPIGVQIVGPWLEDRTPLKLAELIEREFGG
FKPPKALVD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=512, Percent_Identity=28.7109375, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI222831590, Length=520, Percent_Identity=25.7692307692308, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI166795287, Length=374, Percent_Identity=27.807486631016, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=514, Percent_Identity=28.7937743190661, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17538252, Length=313, Percent_Identity=31.629392971246, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71990152, Length=237, Percent_Identity=32.9113924050633, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=232, Percent_Identity=33.6206896551724, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=26.4444444444444, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=430, Percent_Identity=27.4418604651163, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=538, Percent_Identity=25.092936802974, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=430, Percent_Identity=27.4418604651163, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=514, Percent_Identity=25.6809338521401, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319685, Length=427, Percent_Identity=27.4004683840749, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=450, Percent_Identity=23.5555555555556, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=28.7449392712551, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=28.7449392712551, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI45550774, Length=495, Percent_Identity=29.2929292929293, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=28.5148514851485, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=28.5148514851485, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=28.5148514851485, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=28.5148514851485, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=30.0469483568075, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=30.0469483568075, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24644968, Length=275, Percent_Identity=36.7272727272727, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=27.789046653144, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=27.9761904761905, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24648113, Length=505, Percent_Identity=29.3069306930693, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 51894; Mature: 51763

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASPWSFATVTDLSAALKAKQVSAVELTQDAIGRIERHDDKVNAVCVRDFDRALQSARA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
ADLALSRGGREPLLGIPMTVKESFNVAGLPTTWGFVEQKNFVAAADALAVERVKAAGGVI
HHHHHHCCCCCCEEECCEEHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEE
LGKTNVPVALGDWQSYNEIYGTTGNPYDLGRTPGGSSGGSSAALAAGFGALSLGSDIGGS
EECCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCC
LRVPAHYCGVYAHKPTFGLCPARGHTPPPFPPLPSNRDLSVIGPMARSAADLALLLDVIA
EECCHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
GTDPIFDGIAYQLDLPAARHTALKDFRVLLLDTHPLLPTGAAVHSALQTLADGLTKAGAS
CCCHHHCCEEEEEECCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
VTRSSPLLPDLAETSRVYMRMLLSFLAAGFPADLYAGAQAMAAGLSATDDSLAAERLRGI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VLSHRDWVAADSRRTGLRAQWRELFKSFDAVICPVMPTPAYPHDHHEPQEERRILIDGKP
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHCEEEECCCC
HPYVDQLVWPGIATLPGLPSTALPIAMSDDGLPIGVQIVGPWLEDRTPLKLAELIEREFG
CCHHHHHHCCCHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
GFKPPKALVD
CCCCCHHHCC
>Mature Secondary Structure 
PASPWSFATVTDLSAALKAKQVSAVELTQDAIGRIERHDDKVNAVCVRDFDRALQSARA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
ADLALSRGGREPLLGIPMTVKESFNVAGLPTTWGFVEQKNFVAAADALAVERVKAAGGVI
HHHHHHCCCCCCEEECCEEHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEE
LGKTNVPVALGDWQSYNEIYGTTGNPYDLGRTPGGSSGGSSAALAAGFGALSLGSDIGGS
EECCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCC
LRVPAHYCGVYAHKPTFGLCPARGHTPPPFPPLPSNRDLSVIGPMARSAADLALLLDVIA
EECCHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
GTDPIFDGIAYQLDLPAARHTALKDFRVLLLDTHPLLPTGAAVHSALQTLADGLTKAGAS
CCCHHHCCEEEEEECCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
VTRSSPLLPDLAETSRVYMRMLLSFLAAGFPADLYAGAQAMAAGLSATDDSLAAERLRGI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VLSHRDWVAADSRRTGLRAQWRELFKSFDAVICPVMPTPAYPHDHHEPQEERRILIDGKP
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHCEEEECCCC
HPYVDQLVWPGIATLPGLPSTALPIAMSDDGLPIGVQIVGPWLEDRTPLKLAELIEREFG
CCHHHHHHCCCHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
GFKPPKALVD
CCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA