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The map label for this gene is mdlC [H]

Identifier: 192290196

GI number: 192290196

Start: 1931883

End: 1933511

Strand: Reverse

Name: mdlC [H]

Synonym: Rpal_1799

Alternate gene names: 192290196

Gene position: 1933511-1931883 (Counterclockwise)

Preceding gene: 192290197

Following gene: 192290192

Centisome position: 33.66

GC content: 68.57

Gene sequence:

>1629_bases
TTGCCCGCGAAGAAGTCCAAACAGCCGAGCGCTGCTGTCAGCACCGTCAAATCCGCCACCCTCGATCTGCTCCGCGCCTT
CAAGATCGACAAGGTGTTCGGCAATCCCGGCTCGACCGAGCTGCCGTTCCTCAGTGACTGGCCGGACGATATCGACTACG
TGCTGGCGCTGCAGGAAGCTTCGGCGGTGGCGATGGCCGACGGCTACGCGCAGGCGACGCGCAACGCCGGCTTCGTCAAT
CTGCATTCGGCCGCCGGCGTCGGCAATGCGCTCGGCAACATTTACAGCGCGTTCAAGAACCAGACGCCGCTGGTAATCAC
CGCCGGCCAGCAAGCGCGCAGCCTGCTGCCGCTGCAGGCATTCCTGGGCGCCGAGCGCGCCTCCGAGTTTCCGCGGCCTT
ATGTGAAGTACAGCGTCGAGCCGGCCCGCGCCGAGGACGTGCCCACCGCGATTGCCCGTGCCTACTACGTCGCGATGCAG
CCGCCGTGCGGGCCGACCTTCGTGTCGGTACCGATTGACGATTGGGCGCGGCCGGCGGCGCCGGTGCCACCGCGCACGAT
CACCCGCGAGATCGGCCCGGATCGATCAGCAATGCAGGTGCTGGCCGATACGCTCGCCAACGCCAAGAAGCCGGCACTGG
TGGTCGGGCCAGCGATCGACCGCGCCGCGGCGGTCGATCTGATGGTGCAGCTCGCCGAGCGCACGCGAGCGCCAGTGTGG
GTGTCGCCGTTCTCCGCACGCTGCAGCTTCCCGGAACAGCATCTGCTGTTCGCCGGCTTCCTGCCCGCCTCGCCGGGACA
ACTCTCCGAAACGCTCGGCGCCTACGACGTGATCGTGGTGATCGGCGCGCCGGTGTTCACCTTTCATGTCGAAGGCCACG
CCGCGATCTTCGATGGAAGCTCGAAGCTGTTCCAGATCACCGATGATGCCGAAGCCGCCTCGGTGACGCCGCTCGGCGCC
AGCATCATCGCGACGATGACCCCGGCCCTGACGCTGCTGCTGGAGTTGCTGCCGGAGACCAAGCGCGCCGCACCGCCGGC
CCGCGCGGTGCCGCCTGCACCTCAGCCGGCCGAGCCGATGCCGGTGGAGTATCTGCTGCACACCCTGCGCGCCGCGATGC
CCCAGAGCGCGATGCTGGTCGAGGAAGCGCCGTCGCACCGCCCGGCGATGCAGACATACATGCCGATGCCGGGCCAGGAC
AGTTTCGCCACGATGGCGAGCGGCGGCTTGGGCTGGTCGCTGCCGGCGTCGGTCGGTTTTGCGCTGGCGCATCCGAACCG
CCGCACCGTCTGCCTGATCGGCGACGGCTCGGCGATGTACTCGATCCAGGCGCTGTGGACGGCGGCGCAGCGCAAGCTGC
CGCTGACCGTGGTGGTGCTGAACAACGGCGGCTACGGCGCGATGCGCTCGTTCAGCCAGGTGATGCAGGTGCGGAACGTG
CCCGGGCTGGAGCTGCCCGGGATCGACTTCACCGCTCTGGCGCAATCGCTCGGCTGCGATGCTGTGCGGGTGACGCGCAG
CGAGGAACTGGCGCCGGCGCTGACGCGCGCCCTTGCATGGGACGGCGTCAGCCTGGTCGAAGCGATGCTCGATACGTCGG
TGCCGATGCTCTACGCGCGCAACGGCTGA

Upstream 100 bases:

>100_bases
CTTCGGGCCACACGCACACTCGTGTTGACTCCCCTCGCTGCCTGCGGATTTGATCGGTGCAAACAATAACACCGGCGTCC
GCCGCCCGAGGAAATCGCCT

Downstream 100 bases:

>100_bases
CGCTGCGTCATCAGTTGTCCAGCGCCTCGTACACCAGCTTCTTCAGCTCGCGCTGGATCGGCTGGCGATGCGACGGGGTC
TGCTCCATCAGCACGAAGAA

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MPAKKSKQPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAVAMADGYAQATRNAGFVN
LHSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQAFLGAERASEFPRPYVKYSVEPARAEDVPTAIARAYYVAMQ
PPCGPTFVSVPIDDWARPAAPVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVDLMVQLAERTRAPVW
VSPFSARCSFPEQHLLFAGFLPASPGQLSETLGAYDVIVVIGAPVFTFHVEGHAAIFDGSSKLFQITDDAEAASVTPLGA
SIIATMTPALTLLLELLPETKRAAPPARAVPPAPQPAEPMPVEYLLHTLRAAMPQSAMLVEEAPSHRPAMQTYMPMPGQD
SFATMASGGLGWSLPASVGFALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNV
PGLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEAMLDTSVPMLYARNG

Sequences:

>Translated_542_residues
MPAKKSKQPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAVAMADGYAQATRNAGFVN
LHSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQAFLGAERASEFPRPYVKYSVEPARAEDVPTAIARAYYVAMQ
PPCGPTFVSVPIDDWARPAAPVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVDLMVQLAERTRAPVW
VSPFSARCSFPEQHLLFAGFLPASPGQLSETLGAYDVIVVIGAPVFTFHVEGHAAIFDGSSKLFQITDDAEAASVTPLGA
SIIATMTPALTLLLELLPETKRAAPPARAVPPAPQPAEPMPVEYLLHTLRAAMPQSAMLVEEAPSHRPAMQTYMPMPGQD
SFATMASGGLGWSLPASVGFALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNV
PGLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEAMLDTSVPMLYARNG
>Mature_541_residues
PAKKSKQPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEASAVAMADGYAQATRNAGFVNL
HSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQAFLGAERASEFPRPYVKYSVEPARAEDVPTAIARAYYVAMQP
PCGPTFVSVPIDDWARPAAPVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVDLMVQLAERTRAPVWV
SPFSARCSFPEQHLLFAGFLPASPGQLSETLGAYDVIVVIGAPVFTFHVEGHAAIFDGSSKLFQITDDAEAASVTPLGAS
IIATMTPALTLLLELLPETKRAAPPARAVPPAPQPAEPMPVEYLLHTLRAAMPQSAMLVEEAPSHRPAMQTYMPMPGQDS
FATMASGGLGWSLPASVGFALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNVP
GLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEAMLDTSVPMLYARNG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=23.3511586452763, Blast_Score=103, Evalue=6e-22,
Organism=Escherichia coli, GI87081685, Length=520, Percent_Identity=27.8846153846154, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=25.414364640884, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=24.6823956442831, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1787096, Length=486, Percent_Identity=22.2222222222222, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=24.777183600713, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=490, Percent_Identity=22.8571428571429, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=525, Percent_Identity=22.8571428571429, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=523, Percent_Identity=22.7533460803059, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57464; Mature: 57332

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKKSKQPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEEHHC
SAVAMADGYAQATRNAGFVNLHSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQA
CCHHHHCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
FLGAERASEFPRPYVKYSVEPARAEDVPTAIARAYYVAMQPPCGPTFVSVPIDDWARPAA
HHCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHEEEEECCCCCCEEEEECCHHCCCCCC
PVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVDLMVQLAERTRAPVW
CCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEE
VSPFSARCSFPEQHLLFAGFLPASPGQLSETLGAYDVIVVIGAPVFTFHVEGHAAIFDGS
ECCCCCCCCCCHHHEEEEECCCCCCCHHHHHHCCEEEEEEECCCEEEEEECCCEEEEECC
SKLFQITDDAEAASVTPLGASIIATMTPALTLLLELLPETKRAAPPARAVPPAPQPAEPM
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCC
PVEYLLHTLRAAMPQSAMLVEEAPSHRPAMQTYMPMPGQDSFATMASGGLGWSLPASVGF
CHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCHHHCCE
ALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNV
EEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
PGLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEAMLDTSVPMLYAR
CCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEC
NG
CC
>Mature Secondary Structure 
PAKKSKQPSAAVSTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEEHHC
SAVAMADGYAQATRNAGFVNLHSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQA
CCHHHHCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
FLGAERASEFPRPYVKYSVEPARAEDVPTAIARAYYVAMQPPCGPTFVSVPIDDWARPAA
HHCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHEEEEECCCCCCEEEEECCHHCCCCCC
PVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVDLMVQLAERTRAPVW
CCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEE
VSPFSARCSFPEQHLLFAGFLPASPGQLSETLGAYDVIVVIGAPVFTFHVEGHAAIFDGS
ECCCCCCCCCCHHHEEEEECCCCCCCHHHHHHCCEEEEEEECCCEEEEEECCCEEEEECC
SKLFQITDDAEAASVTPLGASIIATMTPALTLLLELLPETKRAAPPARAVPPAPQPAEPM
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCC
PVEYLLHTLRAAMPQSAMLVEEAPSHRPAMQTYMPMPGQDSFATMASGGLGWSLPASVGF
CHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCHHHCCE
ALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNV
EEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
PGLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEAMLDTSVPMLYAR
CCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEC
NG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]