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The map label for this gene is rpoH [H]

Identifier: 192289929

GI number: 192289929

Start: 1606239

End: 1607219

Strand: Reverse

Name: rpoH [H]

Synonym: Rpal_1525

Alternate gene names: 192289929

Gene position: 1607219-1606239 (Counterclockwise)

Preceding gene: 192289930

Following gene: 192289928

Centisome position: 27.98

GC content: 65.95

Gene sequence:

>981_bases
ATGGCCTCATCGTTTCCTATGGCGGCGCCCGCATCGACACACGTCAATTCCGTGCTGTTCGACGAGCAAGACTACATGCG
GGCGATCGGCCGCTATCCGGTGCTCGAACCGGACGAAGAGGCGCGGTTGTGGCAGCGCTGGCACACCGCTCGCGACAAAG
CGGCGGCTGACGTGCTGATCACCAGCCATCTCCGACTCGCGGCCAAGCTGGCCCGCAACTACCGACGCTACGGATTCCCG
ATCGGCGACCTGATCGCCGAAGCCAATCTCGGCCTGATGATGGCGCTCGACAAATTCGATCCGACGCGCGGCGCACGCTT
TGCAACCTGCGCGGTGTGGTGGATCCGGTCGGCGATCTACGATCACATCGTCAGGTCGTGGTCGCTGGTGCGCATCGGTC
GAACGCCGGCGCAGAAGAAGCTGTTCTTCCGTCTCCGCGGCGAGATCCGCCGGCTGAGGCCCGACCACCACGGCGCCCTC
ACCAAGGACCTGGCGGAGCAGATCTCGACCTCACTCGATGTCCCGCTGCGAGAGGTCATCGAGATGGAGCAGCGCCTGTC
GGGCGATCTGTCGTTGAACGCACCGCTGTCGGACCTTGACGAGAGCGGCGAGTGGCAGGATTTGATCGCCGATGACTCGC
CGAATGCCGAAGCTATTCTGGCAGGCCACGACGAGCTCGATCGTCAGCGCGGCGCGCTCCGGGACGCGCTGGTGCAGCTC
GACGCGCGTGAGCGCTACATCTTTTCGGCGCGGCATCTGGGCGACCGCCCCGCCAGCTTCGAGGCCATCGGTCAGTCGCT
CTCGCTTTCTGCCGAACGGGTCCGGCAGATCGAGGCGCGTGCGTTCGCCAAGGTGGCGACAGCGGCGCGGCGGCATTGCG
GCACGGCCCAGCAAGTTGCCCGCGGCAGGATCGACCGACATCCGCATCCGGCCATCCAGAGCCGCCAACTTGGAATGGAG
CAGATGGCGCACGCCTCGTGA

Upstream 100 bases:

>100_bases
ATTCGTTCCGCCGCCCCCTTAACACCTAGATTTAATGTTCAGTGGAAGCGGGCCTCGCTACCTTGCTTACTAGAGTTCAG
CATCGGTAGTGGAGATTACC

Downstream 100 bases:

>100_bases
CGAATGGACCGCGCCCAACCGTTCGGAATGAGCACCGCTCATCCTGCACTCCGGCCGACGAACCGCCGAGTAACCCTCAG
CGTGTCGACAGGCGGCCCCG

Product: RNA polymerase, sigma 32 subunit, RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MASSFPMAAPASTHVNSVLFDEQDYMRAIGRYPVLEPDEEARLWQRWHTARDKAAADVLITSHLRLAAKLARNYRRYGFP
IGDLIAEANLGLMMALDKFDPTRGARFATCAVWWIRSAIYDHIVRSWSLVRIGRTPAQKKLFFRLRGEIRRLRPDHHGAL
TKDLAEQISTSLDVPLREVIEMEQRLSGDLSLNAPLSDLDESGEWQDLIADDSPNAEAILAGHDELDRQRGALRDALVQL
DARERYIFSARHLGDRPASFEAIGQSLSLSAERVRQIEARAFAKVATAARRHCGTAQQVARGRIDRHPHPAIQSRQLGME
QMAHAS

Sequences:

>Translated_326_residues
MASSFPMAAPASTHVNSVLFDEQDYMRAIGRYPVLEPDEEARLWQRWHTARDKAAADVLITSHLRLAAKLARNYRRYGFP
IGDLIAEANLGLMMALDKFDPTRGARFATCAVWWIRSAIYDHIVRSWSLVRIGRTPAQKKLFFRLRGEIRRLRPDHHGAL
TKDLAEQISTSLDVPLREVIEMEQRLSGDLSLNAPLSDLDESGEWQDLIADDSPNAEAILAGHDELDRQRGALRDALVQL
DARERYIFSARHLGDRPASFEAIGQSLSLSAERVRQIEARAFAKVATAARRHCGTAQQVARGRIDRHPHPAIQSRQLGME
QMAHAS
>Mature_325_residues
ASSFPMAAPASTHVNSVLFDEQDYMRAIGRYPVLEPDEEARLWQRWHTARDKAAADVLITSHLRLAAKLARNYRRYGFPI
GDLIAEANLGLMMALDKFDPTRGARFATCAVWWIRSAIYDHIVRSWSLVRIGRTPAQKKLFFRLRGEIRRLRPDHHGALT
KDLAEQISTSLDVPLREVIEMEQRLSGDLSLNAPLSDLDESGEWQDLIADDSPNAEAILAGHDELDRQRGALRDALVQLD
ARERYIFSARHLGDRPASFEAIGQSLSLSAERVRQIEARAFAKVATAARRHCGTAQQVARGRIDRHPHPAIQSRQLGMEQ
MAHAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=36.2007168458781, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=31.7518248175182, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=31.0204081632653, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36727; Mature: 36596

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSFPMAAPASTHVNSVLFDEQDYMRAIGRYPVLEPDEEARLWQRWHTARDKAAADVLI
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSHLRLAAKLARNYRRYGFPIGDLIAEANLGLMMALDKFDPTRGARFATCAVWWIRSAIY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
DHIVRSWSLVRIGRTPAQKKLFFRLRGEIRRLRPDHHGALTKDLAEQISTSLDVPLREVI
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
EMEQRLSGDLSLNAPLSDLDESGEWQDLIADDSPNAEAILAGHDELDRQRGALRDALVQL
HHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
DARERYIFSARHLGDRPASFEAIGQSLSLSAERVRQIEARAFAKVATAARRHCGTAQQVA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RGRIDRHPHPAIQSRQLGMEQMAHAS
HCCCCCCCCCCHHHHHHCHHHHHCCC
>Mature Secondary Structure 
ASSFPMAAPASTHVNSVLFDEQDYMRAIGRYPVLEPDEEARLWQRWHTARDKAAADVLI
CCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSHLRLAAKLARNYRRYGFPIGDLIAEANLGLMMALDKFDPTRGARFATCAVWWIRSAIY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
DHIVRSWSLVRIGRTPAQKKLFFRLRGEIRRLRPDHHGALTKDLAEQISTSLDVPLREVI
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
EMEQRLSGDLSLNAPLSDLDESGEWQDLIADDSPNAEAILAGHDELDRQRGALRDALVQL
HHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
DARERYIFSARHLGDRPASFEAIGQSLSLSAERVRQIEARAFAKVATAARRHCGTAQQVA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RGRIDRHPHPAIQSRQLGMEQMAHAS
HCCCCCCCCCCHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]