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The map label for this gene is nadB [H]

Identifier: 192289656

GI number: 192289656

Start: 1322045

End: 1323661

Strand: Reverse

Name: nadB [H]

Synonym: Rpal_1246

Alternate gene names: 192289656

Gene position: 1323661-1322045 (Counterclockwise)

Preceding gene: 192289657

Following gene: 192289655

Centisome position: 23.04

GC content: 71.12

Gene sequence:

>1617_bases
ATGAGCACCACGCCCGAGTTCGCACATCTTGGCGTCCATGGTGACGTCGTTATCGTCGGCGGCGGTCTCGCCGGACTGTT
CTGCGCGCTGAAATTGGCGCCGCGGCCGGTGACGCTGATCAGCGCCGCACCGCTCGGCGAAGGCGCCTCCACTGCCTGGG
CGCAGGGTGGCATCGCCGCGGCTGTCGGCGAAGGCGACAGCGCCGAATCGCATGCGGCCGACACCATCGCGGTCGGCGCC
GGACTGGTCGACGAGGCGGTGGCGCATCGCCTCGCCGGCGAAGCCGCCGCACGCATCCACGATCTCCTCAGCTACGGCGT
GCCGTTCGACCGCGACCTCGAAGGCCGGCTGGCGGTCGCGCGCGAGGCGGCGCATTCGGCGCGGCGCATCGTCCACGTCC
GCGGCGACATGGCGGGCCAGGCGATCATCAGCGCGCTCACCGAAGCGGTGCGCAACACGCCGTCGATCAAAGTGATCGAA
GGCTGGTTCGCCGACGGGCTGCTGATGCACGACGACGCGGTCGCCGGACTGCGGCTGCGCAAGGTCGGCAACGCCGCCGG
CGCGCCGATGCACATCGCCGCACGTGCCGTGGTGCTCGCCACCGGCGGCATCGGCCATCTGTATGCCGTCACCACCAATC
CGATCGAGGCCCGCGGTGTCGGGCTGGCGATCGCGGCCCGCGCCGGCGCGCGAATCGCCGATCCGGAATTCGTGCAGTTC
CATCCGACCGCCATCATGGTCGGTCGCGATCCGGCGCCGCTCGCCACCGAGGCGCTGCGCGGCGAAGGCGCGATCCTGAT
CAACGGCCAAGGCGAACGCTTCATGCTGGCCGCGCATCCGCTCGCCGAGCTGGCGCCGCGCGACATCGTTGCCCGCGGCG
TGTTTGCCGAAGTCGCCTCCGGCCGCGGCGCCTATCTCGACGCCACCAAGGCGCTTGGCGCGCATTTCGCCGAGCATTTC
CCGACCGTGTACGAGAGCTGCATCTCGGCAGGCATCGATCCGGCGACACAGCCGATCCCGATCGCGCCGGCGGCGCACTA
CCATATGGGCGGCATCGCGGTGGACATGCGTGGCCGCAGTTCGCTGCACGGGCTGTGGGCGGCGGGCGAAGTGTCCTGCA
CCGGCGCGCACGGCGCCAACCGGCTCGCCTCCAACTCGCTACTTGAAGCCGTGGTGTACGCGGCGCGGATCGCCGAGGAC
ATCGCCGGCACCGAACTCGCCGCGACCAAGTCGCGGCTCGATGTGCACGAGACACACAGCACGCCGATCGATCCAGCGCA
GGAGCTGCTGCTGCGCCAGACCATGGCCGGCAAGGTCGGCGTGGTCCGCGACCGCGACGGACTGACCGAAGCGGTGCGAA
CTTTCGCGGCACTCGAAGCCGAGACCTCGTCGACTACGCTCGCCAATATGGCGACGACCGCGCTGCTGGTCGCCACCGCA
GCGCTGGCACGCACCGAAAGCCGGGGCGCGCACTACCGCGCCGACTATCCGACCGAAAACCCGGCGCAGGCGAAACGCAC
CACCACCTCGCTGGCTCAGGCCCGCGAGCGCGCCGCCGCGCTCGGCGGCGGTGCCCCGCGCTCCCCTCGCCTCTCTGCGC
AATCCGCGCTCGCCTGA

Upstream 100 bases:

>100_bases
CAACTGAGCCTCAGGGCTCGCGACGAATGGCGGCGTCCGTGCCGCCATCGCTGTGCATCGAAGGCGTTCGCGGACAATCG
CGTGAGAGTTGGATCGCCGC

Downstream 100 bases:

>100_bases
TTCAGGACCATCTTTCATGTTGCCGTCTGCCCTGCTGCATCCCGATGCATTCCTGTCGCCATTGGCGATCACCGACGCTG
TGCGGCGCGCGCTCGACGAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSTTPEFAHLGVHGDVVIVGGGLAGLFCALKLAPRPVTLISAAPLGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGA
GLVDEAVAHRLAGEAAARIHDLLSYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGQAIISALTEAVRNTPSIKVIE
GWFADGLLMHDDAVAGLRLRKVGNAAGAPMHIAARAVVLATGGIGHLYAVTTNPIEARGVGLAIAARAGARIADPEFVQF
HPTAIMVGRDPAPLATEALRGEGAILINGQGERFMLAAHPLAELAPRDIVARGVFAEVASGRGAYLDATKALGAHFAEHF
PTVYESCISAGIDPATQPIPIAPAAHYHMGGIAVDMRGRSSLHGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAED
IAGTELAATKSRLDVHETHSTPIDPAQELLLRQTMAGKVGVVRDRDGLTEAVRTFAALEAETSSTTLANMATTALLVATA
ALARTESRGAHYRADYPTENPAQAKRTTTSLAQARERAAALGGGAPRSPRLSAQSALA

Sequences:

>Translated_538_residues
MSTTPEFAHLGVHGDVVIVGGGLAGLFCALKLAPRPVTLISAAPLGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGA
GLVDEAVAHRLAGEAAARIHDLLSYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGQAIISALTEAVRNTPSIKVIE
GWFADGLLMHDDAVAGLRLRKVGNAAGAPMHIAARAVVLATGGIGHLYAVTTNPIEARGVGLAIAARAGARIADPEFVQF
HPTAIMVGRDPAPLATEALRGEGAILINGQGERFMLAAHPLAELAPRDIVARGVFAEVASGRGAYLDATKALGAHFAEHF
PTVYESCISAGIDPATQPIPIAPAAHYHMGGIAVDMRGRSSLHGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAED
IAGTELAATKSRLDVHETHSTPIDPAQELLLRQTMAGKVGVVRDRDGLTEAVRTFAALEAETSSTTLANMATTALLVATA
ALARTESRGAHYRADYPTENPAQAKRTTTSLAQARERAAALGGGAPRSPRLSAQSALA
>Mature_537_residues
STTPEFAHLGVHGDVVIVGGGLAGLFCALKLAPRPVTLISAAPLGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGAG
LVDEAVAHRLAGEAAARIHDLLSYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGQAIISALTEAVRNTPSIKVIEG
WFADGLLMHDDAVAGLRLRKVGNAAGAPMHIAARAVVLATGGIGHLYAVTTNPIEARGVGLAIAARAGARIADPEFVQFH
PTAIMVGRDPAPLATEALRGEGAILINGQGERFMLAAHPLAELAPRDIVARGVFAEVASGRGAYLDATKALGAHFAEHFP
TVYESCISAGIDPATQPIPIAPAAHYHMGGIAVDMRGRSSLHGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDI
AGTELAATKSRLDVHETHSTPIDPAQELLLRQTMAGKVGVVRDRDGLTEAVRTFAALEAETSSTTLANMATTALLVATAA
LARTESRGAHYRADYPTENPAQAKRTTTSLAQARERAAALGGGAPRSPRLSAQSALA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=505, Percent_Identity=32.6732673267327, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1788928, Length=535, Percent_Identity=39.6261682242991, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1790597, Length=504, Percent_Identity=31.547619047619, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1786942, Length=516, Percent_Identity=32.1705426356589, Blast_Score=185, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=507, Percent_Identity=31.163708086785, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=528, Percent_Identity=32.0075757575758, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71986328, Length=456, Percent_Identity=29.6052631578947, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322701, Length=525, Percent_Identity=33.3333333333333, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=526, Percent_Identity=33.4600760456274, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320788, Length=488, Percent_Identity=26.844262295082, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137288, Length=505, Percent_Identity=32.8712871287129, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655642, Length=505, Percent_Identity=32.8712871287129, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655647, Length=505, Percent_Identity=32.8712871287129, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24663005, Length=518, Percent_Identity=30.1158301158301, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 55260; Mature: 55129

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPEFAHLGVHGDVVIVGGGLAGLFCALKLAPRPVTLISAAPLGEGASTAWAQGGIAA
CCCCCCCEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCEEE
AVGEGDSAESHAADTIAVGAGLVDEAVAHRLAGEAAARIHDLLSYGVPFDRDLEGRLAVA
EECCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
REAAHSARRIVHVRGDMAGQAIISALTEAVRNTPSIKVIEGWFADGLLMHDDAVAGLRLR
HHHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHHCCHHE
KVGNAAGAPMHIAARAVVLATGGIGHLYAVTTNPIEARGVGLAIAARAGARIADPEFVQF
ECCCCCCCCHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEE
HPTAIMVGRDPAPLATEALRGEGAILINGQGERFMLAAHPLAELAPRDIVARGVFAEVAS
ECCEEEECCCCCCHHHHHHCCCCEEEECCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHC
GRGAYLDATKALGAHFAEHFPTVYESCISAGIDPATQPIPIAPAAHYHMGGIAVDMRGRS
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCC
SLHGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDIAGTELAATKSRLDVHETHS
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCC
TPIDPAQELLLRQTMAGKVGVVRDRDGLTEAVRTFAALEAETSSTTLANMATTALLVATA
CCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALARTESRGAHYRADYPTENPAQAKRTTTSLAQARERAAALGGGAPRSPRLSAQSALA
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
STTPEFAHLGVHGDVVIVGGGLAGLFCALKLAPRPVTLISAAPLGEGASTAWAQGGIAA
CCCCCCEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCEEE
AVGEGDSAESHAADTIAVGAGLVDEAVAHRLAGEAAARIHDLLSYGVPFDRDLEGRLAVA
EECCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
REAAHSARRIVHVRGDMAGQAIISALTEAVRNTPSIKVIEGWFADGLLMHDDAVAGLRLR
HHHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHHCCHHE
KVGNAAGAPMHIAARAVVLATGGIGHLYAVTTNPIEARGVGLAIAARAGARIADPEFVQF
ECCCCCCCCHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEE
HPTAIMVGRDPAPLATEALRGEGAILINGQGERFMLAAHPLAELAPRDIVARGVFAEVAS
ECCEEEECCCCCCHHHHHHCCCCEEEECCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHC
GRGAYLDATKALGAHFAEHFPTVYESCISAGIDPATQPIPIAPAAHYHMGGIAVDMRGRS
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEECCCC
SLHGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDIAGTELAATKSRLDVHETHS
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCC
TPIDPAQELLLRQTMAGKVGVVRDRDGLTEAVRTFAALEAETSSTTLANMATTALLVATA
CCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALARTESRGAHYRADYPTENPAQAKRTTTSLAQARERAAALGGGAPRSPRLSAQSALA
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]