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The map label for this gene is pcaR [H]

Identifier: 192289584

GI number: 192289584

Start: 1244598

End: 1245389

Strand: Reverse

Name: pcaR [H]

Synonym: Rpal_1174

Alternate gene names: 192289584

Gene position: 1245389-1244598 (Counterclockwise)

Preceding gene: 192289592

Following gene: 192289556

Centisome position: 21.68

GC content: 65.66

Gene sequence:

>792_bases
ATGCCCAAACTGAAACGAACCGCCGACGACGGCCCGAGCGAGAGCGCCGAGTTCATCGAAAGCCTGTCGCGCGGCTTACG
GGTGATTGAAGCCTTCGGCGCCGAGAAGCGGCCGATGACGCTGAGCGATGTCGCCAAGGCGTGCGGCCTGCCTCGCGCGA
CGGCGCGACGCATCCTGCTGACGCTGCAAAGCGCCGGCTATGTCAGCAGCGACGACCGGCTGTTCGCGCTGACGCCGCGC
GTCCTGGGTCTCGCATCGGCGTATCTGGCGTCCAACCAGATCAGCGCGGTGCTGCAACCGCTGATGGATCGGGTGTCGGC
GAGCGCCAAGGAAGTTTGCTCGCTGGCGATCCTCGATGGCGACGACGCCGTGTTCGTGGCGCGCGCCAGCCCGGCGCGGG
TGTTCTCGGCCGGGATCGATCTCGGCTATCGGCTGCCGCTGTTCTGTACGTCAGTCGGCCGGGTGCTGCTCAGCCGGCTC
GACAACAACGAACTGACGGCGGCGATCAACGCGATGACGCTGACCAAGCAGACCGAAGACACCATCACCGACAAGCAGAC
GGTGATCGCCACCATCATCGCCGATCGCACCAAAGGCTACTCGCTGGTCGATCGCGAAGCCGAGCCCGGCTTCCGTTCGG
TCGCAGTGCCGATCCGGCGCTATGACGGCACCATTGTCGCCGCCGCCAATATCGGCGCTCATGTCGACCGTATCACCACC
GGCGAGATGATCGACCGCTTCCTGCCGCTGCTGCAGACGATGGCTGAAGAGGCCAGGCCGCTGATGATGTGA

Upstream 100 bases:

>100_bases
AACTTGTGTTCGTTGAGCGAACAATCGTATAGCTCAGCGCTCAAACGTCGCGGCGCCTGCCACTCAGCGCTCCGCCTCAT
TCCCACACCGAAGTACCCGC

Downstream 100 bases:

>100_bases
GTTCGCGGATCGTAGCGAACTGATCATCCCGTCGGCCGCGTCCTGCGTTCGGAAATCGCCCGCCGCAACACGCGCGAAAT
CGCCTCGACCGAATAAGGCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPKLKRTADDGPSESAEFIESLSRGLRVIEAFGAEKRPMTLSDVAKACGLPRATARRILLTLQSAGYVSSDDRLFALTPR
VLGLASAYLASNQISAVLQPLMDRVSASAKEVCSLAILDGDDAVFVARASPARVFSAGIDLGYRLPLFCTSVGRVLLSRL
DNNELTAAINAMTLTKQTEDTITDKQTVIATIIADRTKGYSLVDREAEPGFRSVAVPIRRYDGTIVAAANIGAHVDRITT
GEMIDRFLPLLQTMAEEARPLMM

Sequences:

>Translated_263_residues
MPKLKRTADDGPSESAEFIESLSRGLRVIEAFGAEKRPMTLSDVAKACGLPRATARRILLTLQSAGYVSSDDRLFALTPR
VLGLASAYLASNQISAVLQPLMDRVSASAKEVCSLAILDGDDAVFVARASPARVFSAGIDLGYRLPLFCTSVGRVLLSRL
DNNELTAAINAMTLTKQTEDTITDKQTVIATIIADRTKGYSLVDREAEPGFRSVAVPIRRYDGTIVAAANIGAHVDRITT
GEMIDRFLPLLQTMAEEARPLMM
>Mature_262_residues
PKLKRTADDGPSESAEFIESLSRGLRVIEAFGAEKRPMTLSDVAKACGLPRATARRILLTLQSAGYVSSDDRLFALTPRV
LGLASAYLASNQISAVLQPLMDRVSASAKEVCSLAILDGDDAVFVARASPARVFSAGIDLGYRLPLFCTSVGRVLLSRLD
NNELTAAINAMTLTKQTEDTITDKQTVIATIIADRTKGYSLVDREAEPGFRSVAVPIRRYDGTIVAAANIGAHVDRITTG
EMIDRFLPLLQTMAEEARPLMM

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=230, Percent_Identity=25.2173913043478, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082359, Length=225, Percent_Identity=25.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786715, Length=263, Percent_Identity=26.615969581749, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788131, Length=232, Percent_Identity=26.2931034482759, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789999, Length=239, Percent_Identity=22.5941422594142, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790752, Length=210, Percent_Identity=24.7619047619048, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28376; Mature: 28245

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLKRTADDGPSESAEFIESLSRGLRVIEAFGAEKRPMTLSDVAKACGLPRATARRILL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TLQSAGYVSSDDRLFALTPRVLGLASAYLASNQISAVLQPLMDRVSASAKEVCSLAILDG
HHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
DDAVFVARASPARVFSAGIDLGYRLPLFCTSVGRVLLSRLDNNELTAAINAMTLTKQTED
CCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCHH
TITDKQTVIATIIADRTKGYSLVDREAEPGFRSVAVPIRRYDGTIVAAANIGAHVDRITT
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCH
GEMIDRFLPLLQTMAEEARPLMM
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PKLKRTADDGPSESAEFIESLSRGLRVIEAFGAEKRPMTLSDVAKACGLPRATARRILL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TLQSAGYVSSDDRLFALTPRVLGLASAYLASNQISAVLQPLMDRVSASAKEVCSLAILDG
HHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
DDAVFVARASPARVFSAGIDLGYRLPLFCTSVGRVLLSRLDNNELTAAINAMTLTKQTED
CCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCHH
TITDKQTVIATIIADRTKGYSLVDREAEPGFRSVAVPIRRYDGTIVAAANIGAHVDRITT
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCH
GEMIDRFLPLLQTMAEEARPLMM
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]