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The map label for this gene is algI [H]

Identifier: 192288698

GI number: 192288698

Start: 286231

End: 287643

Strand: Reverse

Name: algI [H]

Synonym: Rpal_0267

Alternate gene names: 192288698

Gene position: 287643-286231 (Counterclockwise)

Preceding gene: 192288702

Following gene: 192288697

Centisome position: 5.01

GC content: 63.48

Gene sequence:

>1413_bases
ATGCTCTTCTACGATCCCCTGTTCCTGACCTGCTTTCCGTTCCTCTATGTGCTGTATTTCGCGGCCCGTGAGGCGCGGAC
CAAGCGCTGGATCCTGCTGATCGGCAGCGGGCTGTTCTACCTCTGGGGCGAGCCGCTGTTCGTGCCGGTGGTGGTGGCCT
CGGCGCTGATCGATCATCTGCTGGCGCGGCGAATCACAGGCAGCTCGAGCGTCCCGACCAAGCGCGCGCTGCTGGCGCTC
GGGGTGGTGCAGAACCTCGCAATCCTGATCATCTATAAATACACCAGTTTTGCGCTCGAGAGCCTGAACGTGCTGCTCGC
CGCCGGCAAGTTCAGTGCGCTCCCGGTCTGGCACATCGCGTTGCCGATCGGCGTCTCGTTCGTCACCTTCGAGAAGATCA
CTTACCTGGTCGACAGCTATCGCGGCGTGTCGCAGCCCGCCCGGCGGTTCTCCGACTACTGCCTGTTCGTGTTCTTCTTT
CCGAAGCTGCTGGCAGGCCCGATCCTCAAATATCATGAGATCGATCGCCAGATCGCTGTGCCACGGGGCTTTGAATGGAG
CGATTGCGGCGCAGGCTTGGCGCGGTTCGCCCAAGGCGCGTTCAAGAAGCTGCTGATCGCCGATCCGCTCGGTCACTCCG
CCGACCAGGTGTTCGGCGCCGATCCGGCGACGCTGTCGATGGGGGGCGCATGGTTAGGTACGGTCTGCTTCACGCTGCAG
ATCTATTTCGACTTCTCCGGCTATTCCGACATGGCGATCGGGATCGCCCGAATGCTCGGCTTCTCGCTGCGCGAGAACTT
CAACATGCCGTACATCTCCCGCTCGATCACCGAGTTCTGGCGACGCTGGCACATCTCGCTCACCACCTGGATCCGCGACT
ATCTGTACATCCCGCTCGGCGGCAACCGCGGCAACGCCCTCGTCACCTATCGCAATCTCTGGATCTGTTTCTTGCTGTCC
GGCCTGTGGCACGGCGCGAGTTGGAATTTCGTGCTGTGGGGCGCCTATAACGGTCTGTTCCTCACGCTCGACCGGGTGTT
CTTGGAGCGTTGGCTGCAACGTGGCGGCGCCGTGATCGCCACCATGGCGACCCTGCTGATCGTGATGCTTGGCTGGGTGA
TCTTCCGCTCGCCGGATACGGCGCACCTCGGTGGTTTCTTCGCTGCGCTCGCCGGCTTTGGTCATGCAACCGCGTGGCTG
CCGCCACCGGTCTCGGTGCTCTGGACGGCCGCGCTCGGCGCAGCGCTGAGCCTGATCCCGGCCCTGCCGGCCTTTGGCTC
GCTGGGCGCAGCCTGGCGCAGCAGTGGTGTGCTTCGGCTCGCCGGCTATGTCGGCCTCGCCGCGCTGGGTACGATCGCGA
TCGCACGTGCGGTGGCGATCCCGTTTCAGCCCTTCATCTATTTCCGGTTCTGA

Upstream 100 bases:

>100_bases
GAGCGGGGCTTCGTGCGTCGCTTCGGCTGGGCGTAGCCTGCGGAATCTGGAATAGGTGGCCCGTCGATCGCCTGCAGGCA
CCAGGAACCACCCGTCCGCC

Downstream 100 bases:

>100_bases
GGATGGCGGCGATTACCACATCACCGCGACGATGGCTCCGCCGGCTGGCGGCCGCTGCCTTTGTGGCGATGCCGGCGTGG
ACGCTTTGGAATCTCGCGGT

Product: membrane bound O-acyl transferase MBOAT family protein

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLFYDPLFLTCFPFLYVLYFAAREARTKRWILLIGSGLFYLWGEPLFVPVVVASALIDHLLARRITGSSSVPTKRALLAL
GVVQNLAILIIYKYTSFALESLNVLLAAGKFSALPVWHIALPIGVSFVTFEKITYLVDSYRGVSQPARRFSDYCLFVFFF
PKLLAGPILKYHEIDRQIAVPRGFEWSDCGAGLARFAQGAFKKLLIADPLGHSADQVFGADPATLSMGGAWLGTVCFTLQ
IYFDFSGYSDMAIGIARMLGFSLRENFNMPYISRSITEFWRRWHISLTTWIRDYLYIPLGGNRGNALVTYRNLWICFLLS
GLWHGASWNFVLWGAYNGLFLTLDRVFLERWLQRGGAVIATMATLLIVMLGWVIFRSPDTAHLGGFFAALAGFGHATAWL
PPPVSVLWTAALGAALSLIPALPAFGSLGAAWRSSGVLRLAGYVGLAALGTIAIARAVAIPFQPFIYFRF

Sequences:

>Translated_470_residues
MLFYDPLFLTCFPFLYVLYFAAREARTKRWILLIGSGLFYLWGEPLFVPVVVASALIDHLLARRITGSSSVPTKRALLAL
GVVQNLAILIIYKYTSFALESLNVLLAAGKFSALPVWHIALPIGVSFVTFEKITYLVDSYRGVSQPARRFSDYCLFVFFF
PKLLAGPILKYHEIDRQIAVPRGFEWSDCGAGLARFAQGAFKKLLIADPLGHSADQVFGADPATLSMGGAWLGTVCFTLQ
IYFDFSGYSDMAIGIARMLGFSLRENFNMPYISRSITEFWRRWHISLTTWIRDYLYIPLGGNRGNALVTYRNLWICFLLS
GLWHGASWNFVLWGAYNGLFLTLDRVFLERWLQRGGAVIATMATLLIVMLGWVIFRSPDTAHLGGFFAALAGFGHATAWL
PPPVSVLWTAALGAALSLIPALPAFGSLGAAWRSSGVLRLAGYVGLAALGTIAIARAVAIPFQPFIYFRF
>Mature_470_residues
MLFYDPLFLTCFPFLYVLYFAAREARTKRWILLIGSGLFYLWGEPLFVPVVVASALIDHLLARRITGSSSVPTKRALLAL
GVVQNLAILIIYKYTSFALESLNVLLAAGKFSALPVWHIALPIGVSFVTFEKITYLVDSYRGVSQPARRFSDYCLFVFFF
PKLLAGPILKYHEIDRQIAVPRGFEWSDCGAGLARFAQGAFKKLLIADPLGHSADQVFGADPATLSMGGAWLGTVCFTLQ
IYFDFSGYSDMAIGIARMLGFSLRENFNMPYISRSITEFWRRWHISLTTWIRDYLYIPLGGNRGNALVTYRNLWICFLLS
GLWHGASWNFVLWGAYNGLFLTLDRVFLERWLQRGGAVIATMATLLIVMLGWVIFRSPDTAHLGGFFAALAGFGHATAWL
PPPVSVLWTAALGAALSLIPALPAFGSLGAAWRSSGVLRLAGYVGLAALGTIAIARAVAIPFQPFIYFRF

Specific function: Together with AlgV and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate increases cyst re

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 52173; Mature: 52173

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFYDPLFLTCFPFLYVLYFAAREARTKRWILLIGSGLFYLWGEPLFVPVVVASALIDHL
CCEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHH
LARRITGSSSVPTKRALLALGVVQNLAILIIYKYTSFALESLNVLLAAGKFSALPVWHIA
HHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEH
LPIGVSFVTFEKITYLVDSYRGVSQPARRFSDYCLFVFFFPKLLAGPILKYHEIDRQIAV
HHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
PRGFEWSDCGAGLARFAQGAFKKLLIADPLGHSADQVFGADPATLSMGGAWLGTVCFTLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEECCCHHHHHHHHEEE
IYFDFSGYSDMAIGIARMLGFSLRENFNMPYISRSITEFWRRWHISLTTWIRDYLYIPLG
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GNRGNALVTYRNLWICFLLSGLWHGASWNFVLWGAYNGLFLTLDRVFLERWLQRGGAVIA
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHH
TMATLLIVMLGWVIFRSPDTAHLGGFFAALAGFGHATAWLPPPVSVLWTAALGAALSLIP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALPAFGSLGAAWRSSGVLRLAGYVGLAALGTIAIARAVAIPFQPFIYFRF
HCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MLFYDPLFLTCFPFLYVLYFAAREARTKRWILLIGSGLFYLWGEPLFVPVVVASALIDHL
CCEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHH
LARRITGSSSVPTKRALLALGVVQNLAILIIYKYTSFALESLNVLLAAGKFSALPVWHIA
HHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEH
LPIGVSFVTFEKITYLVDSYRGVSQPARRFSDYCLFVFFFPKLLAGPILKYHEIDRQIAV
HHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
PRGFEWSDCGAGLARFAQGAFKKLLIADPLGHSADQVFGADPATLSMGGAWLGTVCFTLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEECCCHHHHHHHHEEE
IYFDFSGYSDMAIGIARMLGFSLRENFNMPYISRSITEFWRRWHISLTTWIRDYLYIPLG
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GNRGNALVTYRNLWICFLLSGLWHGASWNFVLWGAYNGLFLTLDRVFLERWLQRGGAVIA
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHH
TMATLLIVMLGWVIFRSPDTAHLGGFFAALAGFGHATAWLPPPVSVLWTAALGAALSLIP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALPAFGSLGAAWRSSGVLRLAGYVGLAALGTIAIARAVAIPFQPFIYFRF
HCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10352233 [H]