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The map label for this gene is fliY [C]

Identifier: 190894721

GI number: 190894721

Start: 409003

End: 409836

Strand: Reverse

Name: fliY [C]

Synonym: RHECIAT_PC0000386

Alternate gene names: 190894721

Gene position: 409836-409003 (Counterclockwise)

Preceding gene: 190894722

Following gene: 190894720

Centisome position: 37.55

GC content: 59.47

Gene sequence:

>834_bases
ATGAACAGACTGACCAAGATGACTGCAGCCGCCGCGTCGCTCGCTATTGTGCTGGCTGCCGTTCCGGCCACTGCCGGCGC
CGTGCTCGACCGCGTGATGGCGGCCAAGGACCTGAAGGTCGCGACCGACGCCAACTGGGCGCCGCAATCCTTCATGAACG
ACAAGAACGAACTTGATGGTTTCGACATCGACGTTGCCAAGGATATCGGCAGGCGTCTCGGTGTGAAGGTCGAATTCGTA
ACGCCCGGCTGGGACATCATCACAGCCGGCAATTGGGCAGGCCGCTGGGACATGCATGTCGGTTCGATGACGCCGACCAA
AAAGCGTGCCGAGATCTTCGACTTCCCCGCCGTTTATTATTACACGCCGGCTGCAGTCGCCGCGCACAAGGACAGCAAGG
CGACCAAGCTCTCCGATTTTGACGGCAAGGTTGTCGGGACCACGGCCACCTCCACCTTCGAAGCCTATGCGAAAGGCGAC
CTGACGCTTGATGCCGCCGGTGCGCCTGCCTTTAAGTACGAGTTCAAGCCGAAGGAAGTGAAGTCCTACGCCAACTCGAC
CACCGCCTTCGATGATCTGCGTCTCGGCGACGGCACCCGTCTCGATGCCGTCGTCTCGTCGCTGCCGAGCATTCTCGAAG
CCGTCAAAGCCGGTTACCCGATCAAGCAGATCGGCGACCCCGTCTTCTACGAGCCGCTCGCCGTTGCCATCGAACATGGC
GACAAGTCGTTCAATGACAAGATCGCCGAAGCCGTGAAGGGCATGCAGTCCGATGGCACGCTGAGCAAGCTTTCGGTAAA
ATGGTATGGCGTGGATTATACGGTCGTAAAATAG

Upstream 100 bases:

>100_bases
TCGCCGGAAGACGAAAAGGTCTCTTCGACTGCAAGATTGCATGACGGTACGGGAATGAGCCCGGGCCAAAACCAAGAATG
ACAATAAAGGGAACCATCGC

Downstream 100 bases:

>100_bases
CCGCCGCCTGACCCAGACGGTCCGGACATCCGGACCGTCGATTTGCCGCGAGGGGCAAGAAATGCTTTATCCCGATACAG
TCGAATCCGAAGTACTCGTC

Product: putative amino acid ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNRLTKMTAAAASLAIVLAAVPATAGAVLDRVMAAKDLKVATDANWAPQSFMNDKNELDGFDIDVAKDIGRRLGVKVEFV
TPGWDIITAGNWAGRWDMHVGSMTPTKKRAEIFDFPAVYYYTPAAVAAHKDSKATKLSDFDGKVVGTTATSTFEAYAKGD
LTLDAAGAPAFKYEFKPKEVKSYANSTTAFDDLRLGDGTRLDAVVSSLPSILEAVKAGYPIKQIGDPVFYEPLAVAIEHG
DKSFNDKIAEAVKGMQSDGTLSKLSVKWYGVDYTVVK

Sequences:

>Translated_277_residues
MNRLTKMTAAAASLAIVLAAVPATAGAVLDRVMAAKDLKVATDANWAPQSFMNDKNELDGFDIDVAKDIGRRLGVKVEFV
TPGWDIITAGNWAGRWDMHVGSMTPTKKRAEIFDFPAVYYYTPAAVAAHKDSKATKLSDFDGKVVGTTATSTFEAYAKGD
LTLDAAGAPAFKYEFKPKEVKSYANSTTAFDDLRLGDGTRLDAVVSSLPSILEAVKAGYPIKQIGDPVFYEPLAVAIEHG
DKSFNDKIAEAVKGMQSDGTLSKLSVKWYGVDYTVVK
>Mature_277_residues
MNRLTKMTAAAASLAIVLAAVPATAGAVLDRVMAAKDLKVATDANWAPQSFMNDKNELDGFDIDVAKDIGRRLGVKVEFV
TPGWDIITAGNWAGRWDMHVGSMTPTKKRAEIFDFPAVYYYTPAAVAAHKDSKATKLSDFDGKVVGTTATSTFEAYAKGD
LTLDAAGAPAFKYEFKPKEVKSYANSTTAFDDLRLGDGTRLDAVVSSLPSILEAVKAGYPIKQIGDPVFYEPLAVAIEHG
DKSFNDKIAEAVKGMQSDGTLSKLSVKWYGVDYTVVK

Specific function: Probably part of a binding-protein-dependent transport system for an amino acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=277, Percent_Identity=24.5487364620939, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787085, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787031, Length=277, Percent_Identity=28.158844765343, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788648, Length=246, Percent_Identity=25.2032520325203, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788649, Length=279, Percent_Identity=26.1648745519713, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=23.4567901234568, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29791; Mature: 29791

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLTKMTAAAASLAIVLAAVPATAGAVLDRVMAAKDLKVATDANWAPQSFMNDKNELDG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCHHCCC
FDIDVAKDIGRRLGVKVEFVTPGWDIITAGNWAGRWDMHVGSMTPTKKRAEIFDFPAVYY
CCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
YTPAAVAAHKDSKATKLSDFDGKVVGTTATSTFEAYAKGDLTLDAAGAPAFKYEFKPKEV
ECCHHHEECCCCCCCEECCCCCEEEEECCCHHHHHHHCCCEEEECCCCCEEEECCCHHHH
KSYANSTTAFDDLRLGDGTRLDAVVSSLPSILEAVKAGYPIKQIGDPVFYEPLAVAIEHG
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHCHHEEEEECC
DKSFNDKIAEAVKGMQSDGTLSKLSVKWYGVDYTVVK
CCCHHHHHHHHHCCCCCCCCEEEEEEEEEECEEEEEC
>Mature Secondary Structure
MNRLTKMTAAAASLAIVLAAVPATAGAVLDRVMAAKDLKVATDANWAPQSFMNDKNELDG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCCHHCCC
FDIDVAKDIGRRLGVKVEFVTPGWDIITAGNWAGRWDMHVGSMTPTKKRAEIFDFPAVYY
CCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
YTPAAVAAHKDSKATKLSDFDGKVVGTTATSTFEAYAKGDLTLDAAGAPAFKYEFKPKEV
ECCHHHEECCCCCCCEECCCCCEEEEECCCHHHHHHHCCCEEEECCCCCEEEECCCHHHH
KSYANSTTAFDDLRLGDGTRLDAVVSSLPSILEAVKAGYPIKQIGDPVFYEPLAVAIEHG
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHCHHEEEEECC
DKSFNDKIAEAVKGMQSDGTLSKLSVKWYGVDYTVVK
CCCHHHHHHHHHCCCCCCCCEEEEEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]