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The map label for this gene is nuoB [H]

Identifier: 190571252

GI number: 190571252

Start: 927800

End: 928303

Strand: Direct

Name: nuoB [H]

Synonym: WPa_0853

Alternate gene names: 190571252

Gene position: 927800-928303 (Clockwise)

Preceding gene: 190571251

Following gene: 190571253

Centisome position: 62.59

GC content: 39.29

Gene sequence:

>504_bases
ATGGGAATAAATTTATCTAATGGCGACTGGAATCGTTACAAAAAAGAAGGTTTTCTTATCACTAAATTTGGCGATTTAAT
AGATTATATAATGAACTGGGCAAGGTCTGGTTCATTGTGGCCAATGACATTTGGTCTTGCATGTTGCGCAGTAGAGATGA
TGCACACTGCATCCAGTCGTTATGATCTTGATAGATATGGCATAATCTTTCGTGCAAGCCCAAGGCAAGCAGATGTTATG
ATTGTTGCTGGCACGTTAACTAATAAAATGGCAGCAGCATTGCGTAAAGTTTATGATCAAATGGCAGATCCCAAATACGT
CGTCTCTATGGGAAGTTGTGCAAATGGCGGTGGCTATTATCATTATTCTTACTCAGTAGTCCGTGGTTGCGATAGAATTG
TGCCAGTTGATGTGTATGTTCCTGGATGCCCTCCAACTGCTGAGGCGTTGCTATATGGAATGCTATGTCTACAAAATAAA
ATAAAACGAACTAAAAATATATAG

Upstream 100 bases:

>100_bases
CTTTATCTAAAATAAGCTATTGCGGATTTTGGTCTATGATGATATTCCTTGCAATACTTACTATAGGCTTTCTTTATGAG
TGGTGTAAAGGTGCGTTGGA

Downstream 100 bases:

>100_bases
CATGGATAAAACTGCAAAATACATACAAAAAAAGACTAAATGTGAATGTATTCAGAAAAATGATGGCACTATTGTAATAT
ATTCAGCTTTGAATGACATT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MGINLSNGDWNRYKKEGFLITKFGDLIDYIMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIIFRASPRQADVM
IVAGTLTNKMAAALRKVYDQMADPKYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNK
IKRTKNI

Sequences:

>Translated_167_residues
MGINLSNGDWNRYKKEGFLITKFGDLIDYIMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIIFRASPRQADVM
IVAGTLTNKMAAALRKVYDQMADPKYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNK
IKRTKNI
>Mature_166_residues
GINLSNGDWNRYKKEGFLITKFGDLIDYIMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIIFRASPRQADVMI
VAGTLTNKMAAALRKVYDQMADPKYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNKI
KRTKNI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=150, Percent_Identity=77.3333333333333, Blast_Score=256, Evalue=5e-69,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=50.6944444444444, Blast_Score=159, Evalue=8e-41,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=44.3396226415094, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=39.6694214876033, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=72.8571428571428, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=78.0141843971631, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=78.0141843971631, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=77.3049645390071, Blast_Score=249, Evalue=9e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18668; Mature: 18537

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGINLSNGDWNRYKKEGFLITKFGDLIDYIMNWARSGSLWPMTFGLACCAVEMMHTASSR
CCCCCCCCCCCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
YDLDRYGIIFRASPRQADVMIVAGTLTNKMAAALRKVYDQMADPKYVVSMGSCANGGGYY
CCHHHCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
HYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNKIKRTKNI
EEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GINLSNGDWNRYKKEGFLITKFGDLIDYIMNWARSGSLWPMTFGLACCAVEMMHTASSR
CCCCCCCCCCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
YDLDRYGIIFRASPRQADVMIVAGTLTNKMAAALRKVYDQMADPKYVVSMGSCANGGGYY
CCHHHCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
HYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNKIKRTKNI
EEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA