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The map label for this gene is dnaB [H]

Identifier: 190570590

GI number: 190570590

Start: 133880

End: 135322

Strand: Direct

Name: dnaB [H]

Synonym: WPa_0128

Alternate gene names: 190570590

Gene position: 133880-135322 (Clockwise)

Preceding gene: 190570586

Following gene: 190570591

Centisome position: 9.03

GC content: 36.04

Gene sequence:

>1443_bases
ATGAATGAATTAGTTAACCTTATTAATTCAAGAGAAGAAATATGTAAATTGCCACATAATTTAGAAGCAGAGCAAATGCT
CATTGGTGCAATGATTCGTGATAATAGAATTTGTGATGCAGTTGAAGATATAATTGCAGCAGACAATTTCTACGATCCAC
TACATCAAAGCATTTTTACCCAGATATCTAAAACCAGAAAACACGGCATAGTTGCCAATGAACTAAGCCTTAAGATGTTT
TTTGAAAATGATAAAGCATTTGCCGAATGTGGTGGAGTTGAGTACCTAGCAAAACTTGCAGCAAAGGCAAGCATTGCGCT
TGACATCTATAGCTTGACCAGAATAATCCGTGATACTTATTTAAGAAGATGCTTAATCAAGCTCGGACAAGAGACAATTG
ACGATAGTTATAACTATGACATCGAAAATCCTGCACAGGTACAAATTGAGCAAGCAATGACAAAATTGTTCAATTTAGCA
GTAAAAAAGCAAGGTGAGAAAACATACATAAAGCTTGCAAGTTCAATTAAAGATGTGGTTGAGAAGATTAGCAAGCTGAA
GAATAACCCAGAGGCTCTAGGTATTACAACCGGACTTCAAGATCTAAACCAGCTTCTTGGTGGTTTGCAGAAATCTGATC
TGTTAATTATAGCTGCAAGGCCCTCAATGGGAAAGACAGCATTGGCACTGAACATCGCACTTAACGCTTGTAAGATTTTG
CAAAAAAGAACAGATAAACAACATTACGTAGCTTTTTTCTCACTTGAAATGTCAGCAGAGCAATTAACCGCAAGATTGAT
TACTATAGATTCAGGCATTGGTTATTATAAGGCACTGACTGGGAGAATTAGTGATTTTGAATTACATGAATTCATTAATG
CTAGTACAGATCTATCAGAATTACCTTTCATAATAGACGATACTCCTGCTCTATCAATTAGTGCCCTTCGTACCAGAATA
CGACTGCTATATCAACTATACAATGTAGAGGTGGTATTTATAGATTATCTGCAATTAATTCGAGGAACAACAAAAAGGAG
TAGTGAAAATAGAGTGCAAGAAATCTCAGAAGTGACGCAGGGTTTAAAAGCAATTGCAAAGGAACTAAATATTCCCATTG
TTGCACTATCACAGCTCTCTCGTTCTGTTGAGCAAAGGGATGATAAAAAACCACAACTTTCTGATTTACGTGATTCAGGA
AGCATAGAGCAGGATGCAGATATAGTAATGTTCCTCTATAGAGAAGAATATTATGAATTAAGAAAGCAACCAAATGAAGG
AAGTAATAAACATCGAGAGTGGCAAGAGAAAATGGAGAAAATTAGAAATATTGCAGAGCTGATCATTGCAAAACAAAGAA
ACGGGCCAATTGGCAGTGTGAAGCTACATTTTGACTCCAATAGAGGTGCATTTAAAGATTATACAGAAAGATATAGTCTG
TAG

Upstream 100 bases:

>100_bases
ATTTTAATGCTATAAGCAACACAGGATTAGTCAAATTTTTTCTCTTTTAAGTCGAGATTGAATATTCTACAATAATCAAT
CATTACTCTAAAAACTTAAA

Downstream 100 bases:

>100_bases
CGGTTTTGAGTAATTCTGGAAGTGTTTAACAAACCATACGTGCCAAATTAAGAAGCTATCGTGAAACATAGCAATATTTA
AGATTGGCATTTTATTTGTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNELVNLINSREEICKLPHNLEAEQMLIGAMIRDNRICDAVEDIIAADNFYDPLHQSIFTQISKTRKHGIVANELSLKMF
FENDKAFAECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQETIDDSYNYDIENPAQVQIEQAMTKLFNLA
VKKQGEKTYIKLASSIKDVVEKISKLKNNPEALGITTGLQDLNQLLGGLQKSDLLIIAARPSMGKTALALNIALNACKIL
QKRTDKQHYVAFFSLEMSAEQLTARLITIDSGIGYYKALTGRISDFELHEFINASTDLSELPFIIDDTPALSISALRTRI
RLLYQLYNVEVVFIDYLQLIRGTTKRSSENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSG
SIEQDADIVMFLYREEYYELRKQPNEGSNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLHFDSNRGAFKDYTERYSL

Sequences:

>Translated_480_residues
MNELVNLINSREEICKLPHNLEAEQMLIGAMIRDNRICDAVEDIIAADNFYDPLHQSIFTQISKTRKHGIVANELSLKMF
FENDKAFAECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQETIDDSYNYDIENPAQVQIEQAMTKLFNLA
VKKQGEKTYIKLASSIKDVVEKISKLKNNPEALGITTGLQDLNQLLGGLQKSDLLIIAARPSMGKTALALNIALNACKIL
QKRTDKQHYVAFFSLEMSAEQLTARLITIDSGIGYYKALTGRISDFELHEFINASTDLSELPFIIDDTPALSISALRTRI
RLLYQLYNVEVVFIDYLQLIRGTTKRSSENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSG
SIEQDADIVMFLYREEYYELRKQPNEGSNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLHFDSNRGAFKDYTERYSL
>Mature_480_residues
MNELVNLINSREEICKLPHNLEAEQMLIGAMIRDNRICDAVEDIIAADNFYDPLHQSIFTQISKTRKHGIVANELSLKMF
FENDKAFAECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQETIDDSYNYDIENPAQVQIEQAMTKLFNLA
VKKQGEKTYIKLASSIKDVVEKISKLKNNPEALGITTGLQDLNQLLGGLQKSDLLIIAARPSMGKTALALNIALNACKIL
QKRTDKQHYVAFFSLEMSAEQLTARLITIDSGIGYYKALTGRISDFELHEFINASTDLSELPFIIDDTPALSISALRTRI
RLLYQLYNVEVVFIDYLQLIRGTTKRSSENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSG
SIEQDADIVMFLYREEYYELRKQPNEGSNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLHFDSNRGAFKDYTERYSL

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=37.1244635193133, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54553; Mature: 54553

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELVNLINSREEICKLPHNLEAEQMLIGAMIRDNRICDAVEDIIAADNFYDPLHQSIFT
CHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
QISKTRKHGIVANELSLKMFFENDKAFAECGGVEYLAKLAAKASIALDIYSLTRIIRDTY
HHHHHHHCCCEEECEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRCLIKLGQETIDDSYNYDIENPAQVQIEQAMTKLFNLAVKKQGEKTYIKLASSIKDVV
HHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKISKLKNNPEALGITTGLQDLNQLLGGLQKSDLLIIAARPSMGKTALALNIALNACKIL
HHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
QKRTDKQHYVAFFSLEMSAEQLTARLITIDSGIGYYKALTGRISDFELHEFINASTDLSE
HHHCCCCCEEEEEEEEHHHHHHHHHHEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCHHH
LPFIIDDTPALSISALRTRIRLLYQLYNVEVVFIDYLQLIRGTTKRSSENRVQEISEVTQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSGSIEQDADIVMFLYREEYYEL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RKQPNEGSNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLHFDSNRGAFKDYTERYSL
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MNELVNLINSREEICKLPHNLEAEQMLIGAMIRDNRICDAVEDIIAADNFYDPLHQSIFT
CHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
QISKTRKHGIVANELSLKMFFENDKAFAECGGVEYLAKLAAKASIALDIYSLTRIIRDTY
HHHHHHHCCCEEECEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRCLIKLGQETIDDSYNYDIENPAQVQIEQAMTKLFNLAVKKQGEKTYIKLASSIKDVV
HHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKISKLKNNPEALGITTGLQDLNQLLGGLQKSDLLIIAARPSMGKTALALNIALNACKIL
HHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
QKRTDKQHYVAFFSLEMSAEQLTARLITIDSGIGYYKALTGRISDFELHEFINASTDLSE
HHHCCCCCEEEEEEEEHHHHHHHHHHEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCHHH
LPFIIDDTPALSISALRTRIRLLYQLYNVEVVFIDYLQLIRGTTKRSSENRVQEISEVTQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSGSIEQDADIVMFLYREEYYEL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
RKQPNEGSNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLHFDSNRGAFKDYTERYSL
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA