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The map label for this gene is top6B

Identifier: 18977951

GI number: 18977951

Start: 1475037

End: 1476743

Strand: Reverse

Name: top6B

Synonym: PF1579

Alternate gene names: 18977951

Gene position: 1476743-1475037 (Counterclockwise)

Preceding gene: 18977952

Following gene: 18977950

Centisome position: 77.39

GC content: 42.41

Gene sequence:

>1707_bases
ATGGCTGAAGCAAGGGATTTATTTAAGGAGTTTAAAGTTCAGAGTGTAAGTGAATTCTTCAGAAGAAATGCAGCAATGTT
AGGATACACTGGAAGCATAAGGTCGCTAACTACAGTCATTCACGAGGCCGTTACAAATTCCCTTGACGCATGTGAAGAGG
CAGGAATTCTCCCTTATGTTAGAGTGGAAATTGAAGAGCTTGGAAAGGAGCATTATAAAGTCATAGTTGAGGATAATGGA
CCAGGAATTCCAGAAGATTACATTCCTCATGTCTTTGGAAAAATGCTCGCCGGAACAAAGGCTCATAGAAACATTCAGAG
CAGGGGACAGCAGGGAATTGGTATAAGTGGTGCTGTAATGTTTGCTCAAATTACAAGTGGAAAGGCTACCAGGGTAATAA
CCTCCACGGGTGACGATGAAATTGTTGAAGCTTGGGTGAAGATTGACGTTCAGAAAAATGAAGGTAAAATTGTGAAGAAG
ATAAAGCATCCAAATCCCAAAAGGTGGAGAGGAACAAGGATAGAGCTTGAAGTTAAGAACGTTAAATATGTTCGCTCAAA
GCAGGGTGTTTATTGGTATCTAAAGTTAACTGCGATTGCAAATCCTCACGCTCATATAGAATTGGTTGAGCCGGACGGGA
AGTTAATAGTCTTTCCCAGGTCGAGTGAGGACATTCCAGAACCACCAGTGGAAATGAAACCTCATCCTAAGGGTGTAATG
ACGGACGATGTATATACAATGGCCCATAGAAGTAAGAGGAGCACTGTAAGGAGATTCCTAGTAAGTGAATTTTCAAGAAT
AAGTGACAAAAAAGTTGACGAGCTGGTACTGTACATAGCTGCTTTGAGGCTAATAAACAGAGAGGTTACAGATCAAAACC
TCAAGTCTCAACTCATTGAAAGGCTTGCTAATGGGGAGGTAGAGAAAGTTCTCAAGTCTTTTGGGAGAAAGTGGAAGAAA
GTCGTTGAGGAAGTCGAGAAGATAATGGAGAAGCCTCCAGAGAAACTAACCTGGCATGAGGCGGAAGAAATAGTTGAGGC
ATTTAAGCTAATGAAATTCCTGGCTCCTCCTACCTATGGACTGAGGCCAATAGGGGAGGAAAACATAGAGAAAGGACTTT
CATCAATTCTCAGGCCAGAGTTTGTAACGGCAGTGACAAGACCACCAAAGGTATATTCGGGTGGAATTCCCTTCCAGGTA
GAGGTGGGAATAGCATATGGAGGGGAAATTACTAACTCAGAAATTTTGAGATATGCTAACAGGGTGCCGTTGCTGTTTGA
TGCGGGTTCATGTGTAATAACTTCAGCAGTCAGAAGCATAGACTGGAGGAGATATAGGGTAGAGTCCTTTGATAACGCTC
CGCTTGTTGTCCTCGTTAATGTTGTAAGTGTTCACGTGCCCTATACTAGCACTGGCAAGCAGAGCATTGCGAGCATTGAT
GAGATTTACAATGAAATTAGGTTGGCTTTAATGGAAGCTGCTAGAAGGCTTGCAGCATATCTTGGTGGGAAATATAGGAG
GTTATATCAGATTAGGAGAAAGAAAATCTTCGAAAAATACCTTCCTGAGATTGCAAGGTCACTTCACATTTTAACTGGCG
AGCCTGAGGAGAAAATCAAGGAATATTTCCTTAGGTTGATAGAAAGCAAAATCACCGTTGCACAAGAGGGGGTGAGCGAA
GTTGAAGTTGAAGAGGGGGAAGCCTAA

Upstream 100 bases:

>100_bases
AGGCTCTCCCCACGGCACTGTGTATAGGTATTTAGAAAGAAGGAAGAAAGACCTTGAACTTGAAGGATCAATGTATTATG
AAAACTTGTGAGGTGAGCAT

Downstream 100 bases:

>100_bases
AGAAAAATTCTCATATGATCCTCAAAAGGTATTAAAAAAGCTTGAGGATCTTGCTTATAAGATACTAGAGGAGGTTAAAG
CAGGCAAGAATCCATACTTC

Product: DNA topoisomerase VI subunit B

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit B; TopoVI-B

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MAEARDLFKEFKVQSVSEFFRRNAAMLGYTGSIRSLTTVIHEAVTNSLDACEEAGILPYVRVEIEELGKEHYKVIVEDNG
PGIPEDYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGDDEIVEAWVKIDVQKNEGKIVKK
IKHPNPKRWRGTRIELEVKNVKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLIVFPRSSEDIPEPPVEMKPHPKGVM
TDDVYTMAHRSKRSTVRRFLVSEFSRISDKKVDELVLYIAALRLINREVTDQNLKSQLIERLANGEVEKVLKSFGRKWKK
VVEEVEKIMEKPPEKLTWHEAEEIVEAFKLMKFLAPPTYGLRPIGEENIEKGLSSILRPEFVTAVTRPPKVYSGGIPFQV
EVGIAYGGEITNSEILRYANRVPLLFDAGSCVITSAVRSIDWRRYRVESFDNAPLVVLVNVVSVHVPYTSTGKQSIASID
EIYNEIRLALMEAARRLAAYLGGKYRRLYQIRRKKIFEKYLPEIARSLHILTGEPEEKIKEYFLRLIESKITVAQEGVSE
VEVEEGEA

Sequences:

>Translated_568_residues
MAEARDLFKEFKVQSVSEFFRRNAAMLGYTGSIRSLTTVIHEAVTNSLDACEEAGILPYVRVEIEELGKEHYKVIVEDNG
PGIPEDYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGDDEIVEAWVKIDVQKNEGKIVKK
IKHPNPKRWRGTRIELEVKNVKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLIVFPRSSEDIPEPPVEMKPHPKGVM
TDDVYTMAHRSKRSTVRRFLVSEFSRISDKKVDELVLYIAALRLINREVTDQNLKSQLIERLANGEVEKVLKSFGRKWKK
VVEEVEKIMEKPPEKLTWHEAEEIVEAFKLMKFLAPPTYGLRPIGEENIEKGLSSILRPEFVTAVTRPPKVYSGGIPFQV
EVGIAYGGEITNSEILRYANRVPLLFDAGSCVITSAVRSIDWRRYRVESFDNAPLVVLVNVVSVHVPYTSTGKQSIASID
EIYNEIRLALMEAARRLAAYLGGKYRRLYQIRRKKIFEKYLPEIARSLHILTGEPEEKIKEYFLRLIESKITVAQEGVSE
VEVEEGEA
>Mature_567_residues
AEARDLFKEFKVQSVSEFFRRNAAMLGYTGSIRSLTTVIHEAVTNSLDACEEAGILPYVRVEIEELGKEHYKVIVEDNGP
GIPEDYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGDDEIVEAWVKIDVQKNEGKIVKKI
KHPNPKRWRGTRIELEVKNVKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLIVFPRSSEDIPEPPVEMKPHPKGVMT
DDVYTMAHRSKRSTVRRFLVSEFSRISDKKVDELVLYIAALRLINREVTDQNLKSQLIERLANGEVEKVLKSFGRKWKKV
VEEVEKIMEKPPEKLTWHEAEEIVEAFKLMKFLAPPTYGLRPIGEENIEKGLSSILRPEFVTAVTRPPKVYSGGIPFQVE
VGIAYGGEITNSEILRYANRVPLLFDAGSCVITSAVRSIDWRRYRVESFDNAPLVVLVNVVSVHVPYTSTGKQSIASIDE
IYNEIRLALMEAARRLAAYLGGKYRRLYQIRRKKIFEKYLPEIARSLHILTGEPEEKIKEYFLRLIESKITVAQEGVSEV
EVEEGEA

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP

COG id: COG1389

COG function: function code L; DNA topoisomerase VI, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP6B_PYRFU (Q8U0K8)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579308.1
- ProteinModelPortal:   Q8U0K8
- SMR:   Q8U0K8
- EnsemblBacteria:   EBPYRT00000004681
- GeneID:   1469455
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1579
- NMPDR:   fig|186497.1.peg.1626
- GeneTree:   EBGT00050000023177
- HOGENOM:   HBG733142
- OMA:   YTSAGKQ
- ProtClustDB:   PRK04184
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- HAMAP:   MF_00322
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005734
- InterPro:   IPR015320
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF006553
- ProDom:   PD013790
- SMART:   SM00387
- TIGRFAMs:   TIGR01052

Pfam domain/function: PF02518 HATPase_c; PF09239 Topo-VIb_trans; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =5.99.1.3

Molecular weight: Translated: 64430; Mature: 64299

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEARDLFKEFKVQSVSEFFRRNAAMLGYTGSIRSLTTVIHEAVTNSLDACEEAGILPYV
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RVEIEELGKEHYKVIVEDNGPGIPEDYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVM
EHHHHHHCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHEE
FAQITSGKATRVITSTGDDEIVEAWVKIDVQKNEGKIVKKIKHPNPKRWRGTRIELEVKN
EEEECCCCCEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHCCCCCCCCCCCEEEEEECC
VKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLIVFPRSSEDIPEPPVEMKPHPKGVM
HHHHHCCCCEEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
TDDVYTMAHRSKRSTVRRFLVSEFSRISDKKVDELVLYIAALRLINREVTDQNLKSQLIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLANGEVEKVLKSFGRKWKKVVEEVEKIMEKPPEKLTWHEAEEIVEAFKLMKFLAPPTYG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LRPIGEENIEKGLSSILRPEFVTAVTRPPKVYSGGIPFQVEVGIAYGGEITNSEILRYAN
CCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHC
RVPLLFDAGSCVITSAVRSIDWRRYRVESFDNAPLVVLVNVVSVHVPYTSTGKQSIASID
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHEECCCCCCCHHHHHHHH
EIYNEIRLALMEAARRLAAYLGGKYRRLYQIRRKKIFEKYLPEIARSLHILTGEPEEKIK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHH
EYFLRLIESKITVAQEGVSEVEVEEGEA
HHHHHHHHHHHHHHHCCCHHCCCCCCCC
>Mature Secondary Structure 
AEARDLFKEFKVQSVSEFFRRNAAMLGYTGSIRSLTTVIHEAVTNSLDACEEAGILPYV
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RVEIEELGKEHYKVIVEDNGPGIPEDYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVM
EHHHHHHCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHEE
FAQITSGKATRVITSTGDDEIVEAWVKIDVQKNEGKIVKKIKHPNPKRWRGTRIELEVKN
EEEECCCCCEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHCCCCCCCCCCCEEEEEECC
VKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLIVFPRSSEDIPEPPVEMKPHPKGVM
HHHHHCCCCEEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
TDDVYTMAHRSKRSTVRRFLVSEFSRISDKKVDELVLYIAALRLINREVTDQNLKSQLIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLANGEVEKVLKSFGRKWKKVVEEVEKIMEKPPEKLTWHEAEEIVEAFKLMKFLAPPTYG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LRPIGEENIEKGLSSILRPEFVTAVTRPPKVYSGGIPFQVEVGIAYGGEITNSEILRYAN
CCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHC
RVPLLFDAGSCVITSAVRSIDWRRYRVESFDNAPLVVLVNVVSVHVPYTSTGKQSIASID
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHEECCCCCCCHHHHHHHH
EIYNEIRLALMEAARRLAAYLGGKYRRLYQIRRKKIFEKYLPEIARSLHILTGEPEEKIK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHH
EYFLRLIESKITVAQEGVSEVEVEEGEA
HHHHHHHHHHHHHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA