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The map label for this gene is thiC

Identifier: 18977903

GI number: 18977903

Start: 1427923

End: 1429212

Strand: Direct

Name: thiC

Synonym: PF1531

Alternate gene names: 18977903

Gene position: 1427923-1429212 (Clockwise)

Preceding gene: 18977890

Following gene: 18977904

Centisome position: 74.83

GC content: 42.33

Gene sequence:

>1290_bases
ATGACTCAGATGGAAGAAGCCAAAAGAGGAGTAATTACGGAAGAAATGAAGAAGATTGCAAAAATTGAGAAAATAGACCC
AGAAAAATTAAGGAGAAGTGTTGCAAAAGGACATACTGTTATTTTTAGGAATGTAAATCACGATTGGGTTGTTCCGGTGG
CGGTGGGTCAAGGGGTTAGGGTTAAGGTCAACGCAAACATAGGGACTTCAAGGGATATAGTAAACGTGGAAGAAGAGATC
GAAAAGGCAAAGATAGCTGTAAAATATGGAGCCGACACTATAATGGATCTCTCCACGGGAGGGGATCTAGACTTCATTAG
AAGAAAGATAATGAAAGCTGTGAACGTTCCCGTGGGAACAGTGCCAATATACCAAGCTGCAGAAGAAATGCTTGCAAGGG
GAAAGGCCATAATAGAGATGACAGAAGATGATATGTGGAAAGCGGTAGAGAAACACTTTAAAGATGGCGTAGACTTTGTC
ACAATACATGCTGGAGTAACAAAGGAAGTTGTTGAGAAGTTAAAGAGAGTAAATAGGGTAGTTGGAATGGTATCAAGAGG
AGGCACATTTTTGGCAGCATGGATACTTCACTGGGGCGAAGAAAATCCCTTCTATAAAAATTATGAGTACTTACTAGAGC
TTGCAAAGGAGTATGACGTAGTTCTCAGTTTAGGCGATGGTCTTAGGCCTGGAGGGCTTCCCGATGCCGGAGATGAGCTT
CAAATTGCAGAGCTCTACACTATAGGAAGACTAGTAAAGAGGGCTAGGGAATTTGGAGTTCAAACTATGGTAGAAGGGCC
AGGTCATGTGCCAATAGACCAGATACCAACTCATATAAGGCTCATGAAGATTGCAAGTGACAATGCACCGGTTTATGTGC
TTGGTCCAATAGTTACCGATATCTTCCCTGGCTATGACCACATTTCCGCAGCCATTGGAGGTGCAATTGCAGCTTTAAAT
GGAGCAGACTTCCTCTGCTATGTAACTCCAGCTGAGCATTTAGGCCTTCCAACCATAGAGCATGTAAAAGAAGGAGTAAT
AGCAACGAAAATAGCAGCTCATGCAGTAAACCTAACAAGATTTGAGGAAGACTTTAAGATTGACTACCAGATGAGCCTTG
CTAGAGGAAAGCTCAACTGGAAAGAGCAATTTAAGATCGCATTTGACAAAGAGAAATTTATCGAGATAAGAAAAGAGAGG
CCAACAAAGAGTGAAGCGTGCTCCATGTGTGGAGATCTTTGCGCAATTAAGATTGTAAGGGAAATGCTTGGACACAAGGC
AAGAAGCTAA

Upstream 100 bases:

>100_bases
TAATATAAAAACTTTGTTTTTTTCATCATGGAAAATGTTTTAAGTGCCCTACAAAACCAAGTTTTGTATTACAAAAACCC
TATTATCTGGAGGTGCAAAT

Downstream 100 bases:

>100_bases
GTTTGAACAAATGCTAATTTTTCACCTCCAATACTGTGATTTTTTGGATTTTTTAAAGATTAAATATTTAATGTCACTCA
AACTTTATTATCTCTTGGTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTQMEEAKRGVITEEMKKIAKIEKIDPEKLRRSVAKGHTVIFRNVNHDWVVPVAVGQGVRVKVNANIGTSRDIVNVEEEI
EKAKIAVKYGADTIMDLSTGGDLDFIRRKIMKAVNVPVGTVPIYQAAEEMLARGKAIIEMTEDDMWKAVEKHFKDGVDFV
TIHAGVTKEVVEKLKRVNRVVGMVSRGGTFLAAWILHWGEENPFYKNYEYLLELAKEYDVVLSLGDGLRPGGLPDAGDEL
QIAELYTIGRLVKRAREFGVQTMVEGPGHVPIDQIPTHIRLMKIASDNAPVYVLGPIVTDIFPGYDHISAAIGGAIAALN
GADFLCYVTPAEHLGLPTIEHVKEGVIATKIAAHAVNLTRFEEDFKIDYQMSLARGKLNWKEQFKIAFDKEKFIEIRKER
PTKSEACSMCGDLCAIKIVREMLGHKARS

Sequences:

>Translated_429_residues
MTQMEEAKRGVITEEMKKIAKIEKIDPEKLRRSVAKGHTVIFRNVNHDWVVPVAVGQGVRVKVNANIGTSRDIVNVEEEI
EKAKIAVKYGADTIMDLSTGGDLDFIRRKIMKAVNVPVGTVPIYQAAEEMLARGKAIIEMTEDDMWKAVEKHFKDGVDFV
TIHAGVTKEVVEKLKRVNRVVGMVSRGGTFLAAWILHWGEENPFYKNYEYLLELAKEYDVVLSLGDGLRPGGLPDAGDEL
QIAELYTIGRLVKRAREFGVQTMVEGPGHVPIDQIPTHIRLMKIASDNAPVYVLGPIVTDIFPGYDHISAAIGGAIAALN
GADFLCYVTPAEHLGLPTIEHVKEGVIATKIAAHAVNLTRFEEDFKIDYQMSLARGKLNWKEQFKIAFDKEKFIEIRKER
PTKSEACSMCGDLCAIKIVREMLGHKARS
>Mature_428_residues
TQMEEAKRGVITEEMKKIAKIEKIDPEKLRRSVAKGHTVIFRNVNHDWVVPVAVGQGVRVKVNANIGTSRDIVNVEEEIE
KAKIAVKYGADTIMDLSTGGDLDFIRRKIMKAVNVPVGTVPIYQAAEEMLARGKAIIEMTEDDMWKAVEKHFKDGVDFVT
IHAGVTKEVVEKLKRVNRVVGMVSRGGTFLAAWILHWGEENPFYKNYEYLLELAKEYDVVLSLGDGLRPGGLPDAGDELQ
IAELYTIGRLVKRAREFGVQTMVEGPGHVPIDQIPTHIRLMKIASDNAPVYVLGPIVTDIFPGYDHISAAIGGAIAALNG
ADFLCYVTPAEHLGLPTIEHVKEGVIATKIAAHAVNLTRFEEDFKIDYQMSLARGKLNWKEQFKIAFDKEKFIEIRKERP
TKSEACSMCGDLCAIKIVREMLGHKARS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=465, Percent_Identity=41.0752688172043, Blast_Score=327, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_PYRFU (Q8U0Q4)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579260.1
- ProteinModelPortal:   Q8U0Q4
- EnsemblBacteria:   EBPYRT00000004775
- GeneID:   1469407
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1531
- NMPDR:   fig|186497.1.peg.1578
- GeneTree:   EBGT00050000023367
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47776; Mature: 47645

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMEEAKRGVITEEMKKIAKIEKIDPEKLRRSVAKGHTVIFRNVNHDWVVPVAVGQGVR
CCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEE
VKVNANIGTSRDIVNVEEEIEKAKIAVKYGADTIMDLSTGGDLDFIRRKIMKAVNVPVGT
EEEECCCCCCCCCCCHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
VPIYQAAEEMLARGKAIIEMTEDDMWKAVEKHFKDGVDFVTIHAGVTKEVVEKLKRVNRV
CHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
VGMVSRGGTFLAAWILHWGEENPFYKNYEYLLELAKEYDVVLSLGDGLRPGGLPDAGDEL
HHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCE
QIAELYTIGRLVKRAREFGVQTMVEGPGHVPIDQIPTHIRLMKIASDNAPVYVLGPIVTD
EHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCEEEEEEECCCCCEEEECCHHHH
IFPGYDHISAAIGGAIAALNGADFLCYVTPAEHLGLPTIEHVKEGVIATKIAAHAVNLTR
HCCCHHHHHHHHHHHHHEECCCCEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHH
FEEDFKIDYQMSLARGKLNWKEQFKIAFDKEKFIEIRKERPTKSEACSMCGDLCAIKIVR
HHHHHEEEEEEEECCCCCCHHHHEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EMLGHKARS
HHHCCCCCC
>Mature Secondary Structure 
TQMEEAKRGVITEEMKKIAKIEKIDPEKLRRSVAKGHTVIFRNVNHDWVVPVAVGQGVR
CCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEE
VKVNANIGTSRDIVNVEEEIEKAKIAVKYGADTIMDLSTGGDLDFIRRKIMKAVNVPVGT
EEEECCCCCCCCCCCHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
VPIYQAAEEMLARGKAIIEMTEDDMWKAVEKHFKDGVDFVTIHAGVTKEVVEKLKRVNRV
CHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
VGMVSRGGTFLAAWILHWGEENPFYKNYEYLLELAKEYDVVLSLGDGLRPGGLPDAGDEL
HHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCE
QIAELYTIGRLVKRAREFGVQTMVEGPGHVPIDQIPTHIRLMKIASDNAPVYVLGPIVTD
EHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCEEEEEEECCCCCEEEECCHHHH
IFPGYDHISAAIGGAIAALNGADFLCYVTPAEHLGLPTIEHVKEGVIATKIAAHAVNLTR
HCCCHHHHHHHHHHHHHEECCCCEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHH
FEEDFKIDYQMSLARGKLNWKEQFKIAFDKEKFIEIRKERPTKSEACSMCGDLCAIKIVR
HHHHHEEEEEEEECCCCCCHHHHEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EMLGHKARS
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA