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The map label for this gene is thiB [H]

Identifier: 18977890

GI number: 18977890

Start: 1416190

End: 1417230

Strand: Direct

Name: thiB [H]

Synonym: PF1518

Alternate gene names: 18977890

Gene position: 1416190-1417230 (Clockwise)

Preceding gene: 18977886

Following gene: 18977903

Centisome position: 74.21

GC content: 42.36

Gene sequence:

>1041_bases
ATGAACAAGATTGTTACTGGAATCTTGCTTGTCTTAGTTCTAATGGCAAGTGGGTGTATAACCTCCCCAGGTGAGCAGGA
GAAGGTTCTGACAGTATATGCCTATGACAGCCTAGAATACTGGCTAAAGGAGGTAATTCCAGAGTTCGAGGAAGAGTACA
ACGTAAAGGTGAACTTGGTCCTAGTTGGAAGTACGGGAGAACTTGTTAACAGGCTAATTATAGAAAAGGACAACCCAAAG
GCGGATGTTGTAGTTGGTATTGACAACACCTTCATGAGCAAAGCAATAAAGGCGGGTGTTCTAGAAAAATACAAGCCCAG
GAATGTTGATGTTATACCTCAGTGGATAATAGAAAAGTTCGACCCCGAATTTTACTTAACACCATTCGACTATGGCTACA
TAGCATTCAACTACAGAACTGACTTAATCCAAGACTATCCAAAGAGCCTGGACGATTTAACAAAGCCAGAGTGGAAGGGC
AAGATTATAATTGAAGACCCAAGAACGAGTTCTCCAGGATTAGCATTCATGCTCTGGACCATTGCGGTATATAAAGACCA
ATGGCTTGATTATTGGGCAAAGTTGAGGGAAAACGTCCAGATAGTAAAGGGATGGAGTGCAGCCTGGGAAGCTTTTAGTA
AGGGAGAGTATCCAATAGTTCTTAGTTATGCCACTTCACCAGCAGCTTCTGTGTATTATGAAAATAACACGAACATCAGG
GCTATTGAGTTCGAAGAAGGAAACTTTCTCCAGATAGAAGGTGTCGGAATAGTGAAGGGAACTAAAAACAGAGAGCTGGC
AGAGAAGTTTGTAGAGTTCCTAATCAGCGAAAAGGCCCAAGAAAAGTTACCAACAACTCAGTGGATGTACCCCGTAAATA
AGAACGTTAAGCTACCAGAAGTTTACAAGTACGCTCTGGAGGTCAAGAAACCCGTTACCATCGATCCAGCTCTTGTGGCA
GAAAATCTTGATAAGTGGCTAAAGCAGTGGACAGAGGTGGTTGTAGAAGGAAAGAGTCCTGAAGAAGTTAAAGGACAATA
A

Upstream 100 bases:

>100_bases
TTTTATGTAAAAATTCGAGTTTTTTAAAGATTTTTTTGACATTTTTATGTAAATAAAAAGCTTAAACTTCGAACCAGAAA
AGTTTAAGGGGTGAAAGAAA

Downstream 100 bases:

>100_bases
ACCTCCTTATCTTAATCCCTACCTTTGCCCCCTTTCTTATTTTCTCTATCTCCTCTAGGGGTCTTTCAGCTAATATCGTT
ATGTTATCAACCTCTAAATA

Product: thiamin-binding periplasmic protein precursor

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNKIVTGILLVLVLMASGCITSPGEQEKVLTVYAYDSLEYWLKEVIPEFEEEYNVKVNLVLVGSTGELVNRLIIEKDNPK
ADVVVGIDNTFMSKAIKAGVLEKYKPRNVDVIPQWIIEKFDPEFYLTPFDYGYIAFNYRTDLIQDYPKSLDDLTKPEWKG
KIIIEDPRTSSPGLAFMLWTIAVYKDQWLDYWAKLRENVQIVKGWSAAWEAFSKGEYPIVLSYATSPAASVYYENNTNIR
AIEFEEGNFLQIEGVGIVKGTKNRELAEKFVEFLISEKAQEKLPTTQWMYPVNKNVKLPEVYKYALEVKKPVTIDPALVA
ENLDKWLKQWTEVVVEGKSPEEVKGQ

Sequences:

>Translated_346_residues
MNKIVTGILLVLVLMASGCITSPGEQEKVLTVYAYDSLEYWLKEVIPEFEEEYNVKVNLVLVGSTGELVNRLIIEKDNPK
ADVVVGIDNTFMSKAIKAGVLEKYKPRNVDVIPQWIIEKFDPEFYLTPFDYGYIAFNYRTDLIQDYPKSLDDLTKPEWKG
KIIIEDPRTSSPGLAFMLWTIAVYKDQWLDYWAKLRENVQIVKGWSAAWEAFSKGEYPIVLSYATSPAASVYYENNTNIR
AIEFEEGNFLQIEGVGIVKGTKNRELAEKFVEFLISEKAQEKLPTTQWMYPVNKNVKLPEVYKYALEVKKPVTIDPALVA
ENLDKWLKQWTEVVVEGKSPEEVKGQ
>Mature_346_residues
MNKIVTGILLVLVLMASGCITSPGEQEKVLTVYAYDSLEYWLKEVIPEFEEEYNVKVNLVLVGSTGELVNRLIIEKDNPK
ADVVVGIDNTFMSKAIKAGVLEKYKPRNVDVIPQWIIEKFDPEFYLTPFDYGYIAFNYRTDLIQDYPKSLDDLTKPEWKG
KIIIEDPRTSSPGLAFMLWTIAVYKDQWLDYWAKLRENVQIVKGWSAAWEAFSKGEYPIVLSYATSPAASVYYENNTNIR
AIEFEEGNFLQIEGVGIVKGTKNRELAEKFVEFLISEKAQEKLPTTQWMYPVNKNVKLPEVYKYALEVKKPVTIDPALVA
ENLDKWLKQWTEVVVEGKSPEEVKGQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=320, Percent_Identity=38.125, Blast_Score=204, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39680; Mature: 39680

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIVTGILLVLVLMASGCITSPGEQEKVLTVYAYDSLEYWLKEVIPEFEEEYNVKVNLV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
LVGSTGELVNRLIIEKDNPKADVVVGIDNTFMSKAIKAGVLEKYKPRNVDVIPQWIIEKF
EECCCHHHHHHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
DPEFYLTPFDYGYIAFNYRTDLIQDYPKSLDDLTKPEWKGKIIIEDPRTSSPGLAFMLWT
CCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
IAVYKDQWLDYWAKLRENVQIVKGWSAAWEAFSKGEYPIVLSYATSPAASVYYENNTNIR
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEE
AIEFEEGNFLQIEGVGIVKGTKNRELAEKFVEFLISEKAQEKLPTTQWMYPVNKNVKLPE
EEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
VYKYALEVKKPVTIDPALVAENLDKWLKQWTEVVVEGKSPEEVKGQ
HHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
>Mature Secondary Structure
MNKIVTGILLVLVLMASGCITSPGEQEKVLTVYAYDSLEYWLKEVIPEFEEEYNVKVNLV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
LVGSTGELVNRLIIEKDNPKADVVVGIDNTFMSKAIKAGVLEKYKPRNVDVIPQWIIEKF
EECCCHHHHHHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
DPEFYLTPFDYGYIAFNYRTDLIQDYPKSLDDLTKPEWKGKIIIEDPRTSSPGLAFMLWT
CCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
IAVYKDQWLDYWAKLRENVQIVKGWSAAWEAFSKGEYPIVLSYATSPAASVYYENNTNIR
HHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEE
AIEFEEGNFLQIEGVGIVKGTKNRELAEKFVEFLISEKAQEKLPTTQWMYPVNKNVKLPE
EEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
VYKYALEVKKPVTIDPALVAENLDKWLKQWTEVVVEGKSPEEVKGQ
HHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]