Click here to switch to the map view.

The map label for this gene is rnz

Identifier: 18977717

GI number: 18977717

Start: 1264393

End: 1265316

Strand: Direct

Name: rnz

Synonym: PF1345

Alternate gene names: 18977717

Gene position: 1264393-1265316 (Clockwise)

Preceding gene: 18977716

Following gene: 18977718

Centisome position: 66.26

GC content: 46.75

Gene sequence:

>924_bases
ATGATTGAGGTAATCTTCCTGGGAACTGGGGGAATAAAGCCCACTCCCGAGAGAAATGTTCCCTCAATAGCGATTAGGAT
AGGGAGTGAGATAGTCCTCTTCGATGTGGGAGAGGGAACTCTCAGGCAGATGGAAATAGCTGGGCTTAATCCGATGAGGA
TAAAGAGAATTTTCATCACCCACTTCCACGGAGACCACTACTTGGGGTTAGCAGCAATAATCCAAACGATGAACTTGTGG
GATAGGAGAGAGACCCTCCACATCTATGGCCCAGAGAATAGCGGCGAATTCATCTCCAACTTTCTTAAGAGTGGCTACTT
TGCCCCTGGGTTTGATGTTGTTGTCCACGAGATAACCGGAAAGGCTAGGCTTCAGTTCGAAAACTACGAAATTTGGACGT
TTGAAGTTTCTCACGGCATTCCTGCGTTGGGGTATGTTTTCAAGGAGAAAGATCGAAGGGGCAACTTTGACCTTGAAAAA
ATAAAGTCTTTAGGCCTAACCCCTGGGCCTTGGATGAAGGAGCTTGAAAAGAGGAAGATAATAAAAATTGGGGAGAGAGT
TATAAGGCTGAGCGAGGTTACGGGGCCAAAGAAGAGGGGAGCTAAGGTAGTTTACACTGGAGACACTGAGCCAATTGACG
ACATAGTTGAGTTTTCGAAGAGAGCGGATTTGTTAATACACGAGGCAACTTACATATCTAGTGAGCACAGGAAGGACAGC
TATCACACAACCATTGAGGAGGCTTATGAGATGTTCAAGAGCTCTAAGGCGAGGCACTTGGCCTTGTTCCATAGGGCTCC
AAGGTATTCCTATCAAGAGTACGCCGTTGCAGCTAAGGAGTTGTGCCCAGAGGCTTACGTTCCCAGGGACTTCGACAGGG
TTTTTGTGGGAGGTATGGGCAATGTCATATTTAAGGTACGTTAA

Upstream 100 bases:

>100_bases
TTCCAGTGGTGACAAGCAACCAAGCAACGCTTTGGTATGCCCTGAGAGAGATAGACGTTAGGGAGAGCTTACCCCTCGGA
AAGCTCCTAAGGGAGTTCTA

Downstream 100 bases:

>100_bases
GATCATTGGAACAGTTCACGTCTCCCCTGAAAGCGTGAGAGAAGTTAGGGAGACTATAATTAGGGAAAAACCCGATGCTA
TAGCATTAGAGTTAGATTAT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIEVIFLGTGGIKPTPERNVPSIAIRIGSEIVLFDVGEGTLRQMEIAGLNPMRIKRIFITHFHGDHYLGLAAIIQTMNLW
DRRETLHIYGPENSGEFISNFLKSGYFAPGFDVVVHEITGKARLQFENYEIWTFEVSHGIPALGYVFKEKDRRGNFDLEK
IKSLGLTPGPWMKELEKRKIIKIGERVIRLSEVTGPKKRGAKVVYTGDTEPIDDIVEFSKRADLLIHEATYISSEHRKDS
YHTTIEEAYEMFKSSKARHLALFHRAPRYSYQEYAVAAKELCPEAYVPRDFDRVFVGGMGNVIFKVR

Sequences:

>Translated_307_residues
MIEVIFLGTGGIKPTPERNVPSIAIRIGSEIVLFDVGEGTLRQMEIAGLNPMRIKRIFITHFHGDHYLGLAAIIQTMNLW
DRRETLHIYGPENSGEFISNFLKSGYFAPGFDVVVHEITGKARLQFENYEIWTFEVSHGIPALGYVFKEKDRRGNFDLEK
IKSLGLTPGPWMKELEKRKIIKIGERVIRLSEVTGPKKRGAKVVYTGDTEPIDDIVEFSKRADLLIHEATYISSEHRKDS
YHTTIEEAYEMFKSSKARHLALFHRAPRYSYQEYAVAAKELCPEAYVPRDFDRVFVGGMGNVIFKVR
>Mature_307_residues
MIEVIFLGTGGIKPTPERNVPSIAIRIGSEIVLFDVGEGTLRQMEIAGLNPMRIKRIFITHFHGDHYLGLAAIIQTMNLW
DRRETLHIYGPENSGEFISNFLKSGYFAPGFDVVVHEITGKARLQFENYEIWTFEVSHGIPALGYVFKEKDRRGNFDLEK
IKSLGLTPGPWMKELEKRKIIKIGERVIRLSEVTGPKKRGAKVVYTGDTEPIDDIVEFSKRADLLIHEATYISSEHRKDS
YHTTIEEAYEMFKSSKARHLALFHRAPRYSYQEYAVAAKELCPEAYVPRDFDRVFVGGMGNVIFKVR

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=317, Percent_Identity=31.2302839116719, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI87082078, Length=300, Percent_Identity=28.6666666666667, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_PYRFU (Q8U182)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579074.1
- ProteinModelPortal:   Q8U182
- SMR:   Q8U182
- EnsemblBacteria:   EBPYRT00000004109
- GeneID:   1469220
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1345
- NMPDR:   fig|186497.1.peg.1391
- GeneTree:   EBGT00050000023080
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- BRENDA:   3.1.26.11
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 35128; Mature: 35128

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVIFLGTGGIKPTPERNVPSIAIRIGSEIVLFDVGEGTLRQMEIAGLNPMRIKRIFIT
CEEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEEEECCCCHHEEEEEEEE
HFHGDHYLGLAAIIQTMNLWDRRETLHIYGPENSGEFISNFLKSGYFAPGFDVVVHEITG
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHEEEEEECC
KARLQFENYEIWTFEVSHGIPALGYVFKEKDRRGNFDLEKIKSLGLTPGPWMKELEKRKI
CEEEEEECEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
IKIGERVIRLSEVTGPKKRGAKVVYTGDTEPIDDIVEFSKRADLLIHEATYISSEHRKDS
HHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHCCEEEEHHHHHCCHHCCCH
YHTTIEEAYEMFKSSKARHLALFHRAPRYSYQEYAVAAKELCPEAYVPRDFDRVFVGGMG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NVIFKVR
CEEEEEC
>Mature Secondary Structure
MIEVIFLGTGGIKPTPERNVPSIAIRIGSEIVLFDVGEGTLRQMEIAGLNPMRIKRIFIT
CEEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEEEECCCCHHEEEEEEEE
HFHGDHYLGLAAIIQTMNLWDRRETLHIYGPENSGEFISNFLKSGYFAPGFDVVVHEITG
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHEEEEEECC
KARLQFENYEIWTFEVSHGIPALGYVFKEKDRRGNFDLEKIKSLGLTPGPWMKELEKRKI
CEEEEEECEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
IKIGERVIRLSEVTGPKKRGAKVVYTGDTEPIDDIVEFSKRADLLIHEATYISSEHRKDS
HHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHCCEEEEHHHHHCCHHCCCH
YHTTIEEAYEMFKSSKARHLALFHRAPRYSYQEYAVAAKELCPEAYVPRDFDRVFVGGMG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NVIFKVR
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA