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The map label for this gene is benB [H]

Identifier: 189354033

GI number: 189354033

Start: 2361615

End: 2362103

Strand: Reverse

Name: benB [H]

Synonym: BMULJ_05286

Alternate gene names: 189354033

Gene position: 2362103-2361615 (Counterclockwise)

Preceding gene: 189354034

Following gene: 189354032

Centisome position: 95.51

GC content: 61.96

Gene sequence:

>489_bases
ATGAGCTTCGATTACCACACCATCTGCGCGACGCTGTACCGCGAGGCGCGCCTGCTCGACGATCGTCAGTGGGACGAGTG
GCTGACCTGCTACACCGACGACGTCACGTACTGGATGCCCGCGTGGGACGACGACGATCGCCTGACCGACGATCACGAAA
GCCAGATCTCGCTGATGTACTACCCCGATCGCGGCGGCCTCGAGGATCGCGTGTTCCGCATCAAGACCGAGCGCAGCGGC
GCATCGACGCCGGAGCCGCGCACGAGCCACAGCGTGACCAACGTCGAGGTGCTGGCCGAACGCGGCGACGAAGTGGACGT
GCGTTACAACTTCCACACGCTCAATCATCGCTACCGCGTGACCGACCATTTCTTCGGCACGATGTTCGTTACGCTGCGCA
AGGTCGGCGACGCGCTGCTGATCTCGTACAAGAAGATCGTGCTGAAGAACGACTACATCCGCCAGGTGCTCGACGTCTAT
CACGTCTGA

Upstream 100 bases:

>100_bases
GCGAGCGCAGCGAAGACGAAGGGCTGTTCGTGTGCCAGCACGAATACTGGGTGCGCGTGATGCGTGACGCGCTGAAGCAG
GAACGCGAGGAGGCGCTGTC

Downstream 100 bases:

>100_bases
TGCCTGATATCGAAAACGGAATGGAGGAGCGTTCCATGTCCAGCTACAAGATTGCACTGAACTTCGAGGACGGCGTCACG
CGCTTCATCGAATGCAAGGC

Product: 2-chlorobenzoate 1,2-dioxygenase

Products: 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSFDYHTICATLYREARLLDDRQWDEWLTCYTDDVTYWMPAWDDDDRLTDDHESQISLMYYPDRGGLEDRVFRIKTERSG
ASTPEPRTSHSVTNVEVLAERGDEVDVRYNFHTLNHRYRVTDHFFGTMFVTLRKVGDALLISYKKIVLKNDYIRQVLDVY
HV

Sequences:

>Translated_162_residues
MSFDYHTICATLYREARLLDDRQWDEWLTCYTDDVTYWMPAWDDDDRLTDDHESQISLMYYPDRGGLEDRVFRIKTERSG
ASTPEPRTSHSVTNVEVLAERGDEVDVRYNFHTLNHRYRVTDHFFGTMFVTLRKVGDALLISYKKIVLKNDYIRQVLDVY
HV
>Mature_161_residues
SFDYHTICATLYREARLLDDRQWDEWLTCYTDDVTYWMPAWDDDDRLTDDHESQISLMYYPDRGGLEDRVFRIKTERSGA
STPEPRTSHSVTNVEVLAERGDEVDVRYNFHTLNHRYRVTDHFFGTMFVTLRKVGDALLISYKKIVLKNDYIRQVLDVYH
V

Specific function: Unknown

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: 1.14.12.10

Molecular weight: Translated: 19341; Mature: 19210

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDYHTICATLYREARLLDDRQWDEWLTCYTDDVTYWMPAWDDDDRLTDDHESQISLMY
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEE
YPDRGGLEDRVFRIKTERSGASTPEPRTSHSVTNVEVLAERGDEVDVRYNFHTLNHRYRV
ECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHEEEECCCCEEEEEEEEEECCCEEEE
TDHFFGTMFVTLRKVGDALLISYKKIVLKNDYIRQVLDVYHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SFDYHTICATLYREARLLDDRQWDEWLTCYTDDVTYWMPAWDDDDRLTDDHESQISLMY
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEE
YPDRGGLEDRVFRIKTERSGASTPEPRTSHSVTNVEVLAERGDEVDVRYNFHTLNHRYRV
ECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHEEEECCCCEEEEEEEEEECCCEEEE
TDHFFGTMFVTLRKVGDALLISYKKIVLKNDYIRQVLDVYHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzoate; NADH; H+; O2

Specific reaction: benzoate + NADH + H+ + O2 = 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938949 [H]