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The map label for this gene is benA [H]

Identifier: 189354034

GI number: 189354034

Start: 2362100

End: 2363458

Strand: Reverse

Name: benA [H]

Synonym: BMULJ_05287

Alternate gene names: 189354034

Gene position: 2363458-2362100 (Counterclockwise)

Preceding gene: 189354035

Following gene: 189354033

Centisome position: 95.56

GC content: 63.43

Gene sequence:

>1359_bases
ATGTCCGCCAATTTCGACCAAGCGAACGCACTCGACCAACTGCTGGCGTCCGCCGTACAGGACGACAAGGCCGCCGGCCT
GTACCGCTGCCGCCGCGACATCTTCACGAACGACGCGCTGTTCGAGCTCGAGATGAAGCACATCTTCGAGCGCAACTGGG
TGTATCTCGCGCACGAAAGCCAGATCCCGAACAACAACGACTACTACACGACATGGATCGGCCGCCAGCCGATCGTGATC
ACGCGCGACAAGAACGGCGAGCTGCATGCCGTGATCAACGCGTGCGCGCACAAGGGCGCGATGCTGTGCCGCCGCAAGCA
CGGCAACAAGGGCAGCTTCACGTGCCCGTTCCACGGCTGGACGTTCTCGAACAGCGGCAAGCTGCTGAAGGTGAAGGACG
AGAAGACGACCGAGTATCCGGTGCAGTTCAACACGAACGGCTCGCACGACCTGAAGAAGGTCGCGCGCTTCGAGAACTAT
CGCGGCTTCCTGTTCGGCAGCCTCAATGCCGACGTGCTGCCGCTCGAAGACTATCTCGGCGAAGCGCGCGTGATCATCGA
CCAGATCGTCGACCAGGCGCCCAACGGCCTCGAAGTGCTGCGCGGCAATTCGTCCTACATCTACGAGGGCAACTGGAAGA
TGCAGATGGAGAACGGCTGCGACGGCTATCACGTCAGCACCGTTCACTGGAACTACGCGGCGACGATGGGCCGGCGCAAG
GAAGACGGCACGAAGGCGGTCGACGCGAACAGCTGGAGCCGCTCGGTGGCCGGCGTGTACGGCTTCGACCACGGCCACAT
CCTGCTGTGGACGAAGACGATGAACCCGGAAGTGCGTCCCGTCTACCGGCACCGCGACGAGATTCGTGCGCGCGTCGGCG
AGGTGCGCGCCGATTTCATCGTGAACCAGACGCGCAACCTGTGCGTGTATCCGAACGTGTTCCTGATGGACCAGTTCAGC
ACGCAGATTCGCGTCGTGCGGCCGCTCGCCGTCGACAAGACCGAAGTGACGATCTTCTGCTTCGCGCCGAAGGGCGAAAG
CGCGGAAGACCGCGCGCTGCGCATCCGCCAGTACGAGGACTTCTTCAACGTGTCGGGCATGGGCACCGCGGACGATCTCG
AGGAGTTTCGCGCATGCCAGGCCGGCTATGCGGGCATCACGTCGCTGTGGAACGACCTGTCGCGCGGCGCGCCGCTCTGG
ATCGAAGGGCCGGACGAGAACGCGACGAAGATGGGGCTGAAGCCGCTGATCTCGGGCGAGCGCAGCGAAGACGAAGGGCT
GTTCGTGTGCCAGCACGAATACTGGGTGCGCGTGATGCGTGACGCGCTGAAGCAGGAACGCGAGGAGGCGCTGTCATGA

Upstream 100 bases:

>100_bases
CGCGTAACGCCCCGGCGCCGCGGCGGCGCTGCGCCGCGCGGCTCCCGCGCCCCCTCTTTCGACACCGATGCACGCCGGCG
CGTGCATGGGAGGAACCACC

Downstream 100 bases:

>100_bases
GCTTCGATTACCACACCATCTGCGCGACGCTGTACCGCGAGGCGCGCCTGCTCGACGATCGTCAGTGGGACGAGTGGCTG
ACCTGCTACACCGACGACGT

Product: benzoate 1,2-dioxygenase alpha subunit

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSANFDQANALDQLLASAVQDDKAAGLYRCRRDIFTNDALFELEMKHIFERNWVYLAHESQIPNNNDYYTTWIGRQPIVI
TRDKNGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVQFNTNGSHDLKKVARFENY
RGFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
EDGTKAVDANSWSRSVAGVYGFDHGHILLWTKTMNPEVRPVYRHRDEIRARVGEVRADFIVNQTRNLCVYPNVFLMDQFS
TQIRVVRPLAVDKTEVTIFCFAPKGESAEDRALRIRQYEDFFNVSGMGTADDLEEFRACQAGYAGITSLWNDLSRGAPLW
IEGPDENATKMGLKPLISGERSEDEGLFVCQHEYWVRVMRDALKQEREEALS

Sequences:

>Translated_452_residues
MSANFDQANALDQLLASAVQDDKAAGLYRCRRDIFTNDALFELEMKHIFERNWVYLAHESQIPNNNDYYTTWIGRQPIVI
TRDKNGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVQFNTNGSHDLKKVARFENY
RGFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
EDGTKAVDANSWSRSVAGVYGFDHGHILLWTKTMNPEVRPVYRHRDEIRARVGEVRADFIVNQTRNLCVYPNVFLMDQFS
TQIRVVRPLAVDKTEVTIFCFAPKGESAEDRALRIRQYEDFFNVSGMGTADDLEEFRACQAGYAGITSLWNDLSRGAPLW
IEGPDENATKMGLKPLISGERSEDEGLFVCQHEYWVRVMRDALKQEREEALS
>Mature_451_residues
SANFDQANALDQLLASAVQDDKAAGLYRCRRDIFTNDALFELEMKHIFERNWVYLAHESQIPNNNDYYTTWIGRQPIVIT
RDKNGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVQFNTNGSHDLKKVARFENYR
GFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRKE
DGTKAVDANSWSRSVAGVYGFDHGHILLWTKTMNPEVRPVYRHRDEIRARVGEVRADFIVNQTRNLCVYPNVFLMDQFST
QIRVVRPLAVDKTEVTIFCFAPKGESAEDRALRIRQYEDFFNVSGMGTADDLEEFRACQAGYAGITSLWNDLSRGAPLWI
EGPDENATKMGLKPLISGERSEDEGLFVCQHEYWVRVMRDALKQEREEALS

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=362, Percent_Identity=29.8342541436464, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788103, Length=204, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 51599; Mature: 51467

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANFDQANALDQLLASAVQDDKAAGLYRCRRDIFTNDALFELEMKHIFERNWVYLAHES
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEEECC
QIPNNNDYYTTWIGRQPIVITRDKNGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGW
CCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCE
TFSNSGKLLKVKDEKTTEYPVQFNTNGSHDLKKVARFENYRGFLFGSLNADVLPLEDYLG
EECCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHC
EARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCEEEEECCCCCCEEEEEEEEEHHHHCCCCC
EDGTKAVDANSWSRSVAGVYGFDHGHILLWTKTMNPEVRPVYRHRDEIRARVGEVRADFI
CCCCCEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VNQTRNLCVYPNVFLMDQFSTQIRVVRPLAVDKTEVTIFCFAPKGESAEDRALRIRQYED
CCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHH
FFNVSGMGTADDLEEFRACQAGYAGITSLWNDLSRGAPLWIEGPDENATKMGLKPLISGE
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHCCHHHCCCC
RSEDEGLFVCQHEYWVRVMRDALKQEREEALS
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SANFDQANALDQLLASAVQDDKAAGLYRCRRDIFTNDALFELEMKHIFERNWVYLAHES
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEEECC
QIPNNNDYYTTWIGRQPIVITRDKNGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGW
CCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCE
TFSNSGKLLKVKDEKTTEYPVQFNTNGSHDLKKVARFENYRGFLFGSLNADVLPLEDYLG
EECCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHC
EARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCEEEEECCCCCCEEEEEEEEEHHHHCCCCC
EDGTKAVDANSWSRSVAGVYGFDHGHILLWTKTMNPEVRPVYRHRDEIRARVGEVRADFI
CCCCCEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VNQTRNLCVYPNVFLMDQFSTQIRVVRPLAVDKTEVTIFCFAPKGESAEDRALRIRQYED
CCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHH
FFNVSGMGTADDLEEFRACQAGYAGITSLWNDLSRGAPLWIEGPDENATKMGLKPLISGE
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHCCHHHCCCC
RSEDEGLFVCQHEYWVRVMRDALKQEREEALS
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]