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The map label for this gene is fadA [H]

Identifier: 189353913

GI number: 189353913

Start: 2226601

End: 2227803

Strand: Reverse

Name: fadA [H]

Synonym: BMULJ_05165

Alternate gene names: 189353913

Gene position: 2227803-2226601 (Counterclockwise)

Preceding gene: 189353915

Following gene: 189353912

Centisome position: 90.08

GC content: 69.08

Gene sequence:

>1203_bases
ATGACCGAAGCTTTTCTGTGTGATGCGATTCGTACGCCGATCGGCCGTTACGGCGGCGCGTTGTCCGGCGTGCGCGCCGA
CGATCTGGGCGCGGTGCCGCTGAAGGCGCTCGTCGAACGCAACCGCGACGTCGACTGGAACGCGATCGACGACGTGATCT
ACGGCTGCGCGAACCAGGCCGGCGAAGACAACCGCAACGTCGCGCGCATGTCGCTGCTGCTCGCAGGGCTGCCGCAAGGC
GTGCCCGGTTCGACGATCAACCGCCTTTGCGGTTCCGGGATGGACGCGGTCGGCGTTGCCGCGCGCGCGATCAAGTCGGG
CGAGGCCGCGCTGATGGTGGCCGGCGGCGTCGAAAGCATGACGCGCGCGCCGTTCGTGATGGGCAAGGCCGCGAGCGCAT
TCGCGCGTCAGGCCGACATCTACGACACGACGATCGGCTGGCGTTTCGTCAATCCGCTGATGAAGCAGCTGCATGGCGTC
GACTCGATGCCGGAGACGGCCGAGAACGTCGCGGTCGACTACAACGTGAGCCGCGCCGATCAGGATCTGTTCGCGCTGCG
CAGCCAGCAGAAGGCTGCGCGCGCGCAGCAGGACGGCACGCTCGCCGAGGAAATCGTATCGGTCACGATTCCGCAGAAGA
AGGGCGACCCGCTCGTCGTGTCGCGCGACGAGCATCCGCGCGAGACCTCGCTCGAAGCGCTCGCGAAGCTGAAGGGCGTC
GTGCGCCCGGACGGTACGGTGACGGCCGGCAACGCGTCGGGCGTCAACGACGGCGCGTGCGCGCTGCTGCTCGCCAACGA
AGCGAGCGCTAAGCGCTTCGGGCTCACGCCGCGTGCGCGCGTGCTCGGCATCGCGACGGCAGGCGTCGCGCCGCGCGTGA
TGGGCATCGGCCCGGCGCCGGCGACGCAGAAGCTGCTCGCGCGCCTCGGGATGACGATCGATCAGTTCGACGTGATCGAA
CTGAACGAAGCGTTCGCGTCGCAGGGCCTCGCCGTGCTGCGCATGCTCGGTGTTGCCGACGACGATCCGCGCGTGAACCC
GAACGGCGGCGCGATCGCGCTCGGCCATCCGCTCGGCGCGTCGGGCGCGCGGCTCGTGACGACGGCGATGTATCAGCTGC
ATCGCACGAACGGCCGCTTCGCGCTGTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATCGCGATCGAACGCGTG
TGA

Upstream 100 bases:

>100_bases
CGCATCCCGGCGGGCAATGCAAGGGGCGCGCCCTGTTCATTTCCCAGCCTTGATGAAGCCGGCGTTCGCGCAGCGCGCTG
CCCGGCGACAGGAGACACTG

Downstream 100 bases:

>100_bases
TCGCGGCGCGCGTCGAAGCGGTTTCAGCAGCCGCTTTGACGCTGCGCGACAGGCAGGGCCGTGGGCATTACGTTGGGTAT
GCCTTCGTCCTTGCCGATAG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFLCDAIRTPIGRYGGALSGVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSLLLAGLPQG
VPGSTINRLCGSGMDAVGVAARAIKSGEAALMVAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLHGV
DSMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVSVTIPQKKGDPLVVSRDEHPRETSLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARVLGIATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIAIAIERV

Sequences:

>Translated_400_residues
MTEAFLCDAIRTPIGRYGGALSGVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSLLLAGLPQG
VPGSTINRLCGSGMDAVGVAARAIKSGEAALMVAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLHGV
DSMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVSVTIPQKKGDPLVVSRDEHPRETSLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARVLGIATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIAIAIERV
>Mature_399_residues
TEAFLCDAIRTPIGRYGGALSGVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSLLLAGLPQGV
PGSTINRLCGSGMDAVGVAARAIKSGEAALMVAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLHGVD
SMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVSVTIPQKKGDPLVVSRDEHPRETSLEALAKLKGVV
RPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARVLGIATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIEL
NEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIAIAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=41.0126582278481, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.5390428211587, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=34.2431761786601, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=38.6934673366834, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.615925058548, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=29.6954314720812, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.8279301745636, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.9057071960298, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.139650872818, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=41.7283950617284, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=32.6086956521739, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=44.2211055276382, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=408, Percent_Identity=32.843137254902, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.0049261083744, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=31.6279069767442, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.3827160493827, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=416, Percent_Identity=36.0576923076923, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=35.4838709677419, Blast_Score=203, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.8902743142145, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=37.2208436724566, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.320987654321, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24585051, Length=358, Percent_Identity=24.8603351955307, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41809; Mature: 41678

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFLCDAIRTPIGRYGGALSGVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQA
CCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
GEDNRNVARMSLLLAGLPQGVPGSTINRLCGSGMDAVGVAARAIKSGEAALMVAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLHGVDSMPETAENVAVDYNVSRAD
HHCCCHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCH
QDLFALRSQQKAARAQQDGTLAEEIVSVTIPQKKGDPLVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARVLGIATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHEEEEEECCCCCCEEECCCCC
ATQKLLARLGMTIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIAIAIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TEAFLCDAIRTPIGRYGGALSGVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQA
CCHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
GEDNRNVARMSLLLAGLPQGVPGSTINRLCGSGMDAVGVAARAIKSGEAALMVAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLHGVDSMPETAENVAVDYNVSRAD
HHCCCHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCH
QDLFALRSQQKAARAQQDGTLAEEIVSVTIPQKKGDPLVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARVLGIATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHEEEEEECCCCCCEEECCCCC
ATQKLLARLGMTIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIAIAIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]