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The map label for this gene is pcaQ [H]

Identifier: 189352856

GI number: 189352856

Start: 982268

End: 983245

Strand: Direct

Name: pcaQ [H]

Synonym: BMULJ_04090

Alternate gene names: 189352856

Gene position: 982268-983245 (Clockwise)

Preceding gene: 189352853

Following gene: 189352860

Centisome position: 39.72

GC content: 72.7

Gene sequence:

>978_bases
ATGAATAATCGAATCGCGGACGGCCGCGTCAAGTTCCGGCATCTGCAGTGCTTTCTCGCGGTCGCGCAGCTGGGCGGCGT
GCAGAAGGCGGCGGAAAGCCTATCGATCACGCAGCCGGCGGTGTCGAAGACGATCGCCGAGCTGGAGGGCATTCTCGGCG
TGAAGCTGTTCGAGCGCGGCCGGCACGGCGCGCAGCCGACGCGCGAAGCGCAACTCTTCATGCCGCATGCGAACGCGTGC
GTGCTCGCATTGCGGCAGGGCGTCGGCTTGCTCGCGCGCGAGGGCGGCGCGGCCGCCGCGACGCTCGAGATCGGCATGCT
GCCGACCGTGGCCGCGTCGCTCGCGCCGGCACTGATGAAGGCGCTGGCCGCACGCTGGCCGCGCGTCGTCGTGCGGATCG
CGACGGCGGCCAATACGGAGCTGCTCGAACGGCTGAAGGCCGGGGCGATCGAATGCGCCATCGGGCGCTTGTCGGAGCCC
GAGCGCATGGTCGGGCTCGCGTTCGAGCAGCTGTACAACGAGCCGCTCGTCGCGGTCGTGCGCGCCGGCCATCCGCTGCT
CGCAAGCGCCGCGCCGGCCGCCGAGCTCGCGCGCTATCCGGTCGTGCTGCCGCCGTACGGCACGCTGATCCGCCAGTCGG
CCGAGCAACTGCTCGGCGCGTGCGGTGCGCCGCCGCTCGATGCGTTCATCGAGGTGCTGTCGGTGTCGGTTGCGCGTGCG
CTCGCGCTCGAGAACGATGCGGTGTGGTTCGTGCCGCTGTATGCGGCCGAGTACGATCTGTCGGCCGGCGCGCTGGCGCG
GCTGCCGCTGCCGGCCGCCGGCACCGACGAGCCGGTCGGGCTGGTGCTGCGCACCGACGCGCAGCCGACGCCCGTCGCGC
GCACGCTGATCGACGCGGTGCGCGATATTGCGCGTGCGCGCTTCGGCGACGCGCGGCCGCGCCGCGCGGCGCGCAAACCC
GCGCGTCGTGGTGGCTGA

Upstream 100 bases:

>100_bases
TGTTTCCGCCACGTGGTCGATCCCGGACCTCGGGCCGCTTCGGTTTTGCCGAAAAGTTATGCAAAATGGCACTTTATCGC
CCATCGCATAACGATCTGTT

Downstream 100 bases:

>100_bases
CGCGCGAGGGTGTCGCGGTCGAGGTTGGATGCGTGGGCGAGGGTGATTGCGTCGCGGAGCCGCGAGTCGTCGGCCGCGAG
CCACGATCTACGAGCCACGA

Product: LysR family pca operon transcriptional activator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNNRIADGRVKFRHLQCFLAVAQLGGVQKAAESLSITQPAVSKTIAELEGILGVKLFERGRHGAQPTREAQLFMPHANAC
VLALRQGVGLLAREGGAAAATLEIGMLPTVAASLAPALMKALAARWPRVVVRIATAANTELLERLKAGAIECAIGRLSEP
ERMVGLAFEQLYNEPLVAVVRAGHPLLASAAPAAELARYPVVLPPYGTLIRQSAEQLLGACGAPPLDAFIEVLSVSVARA
LALENDAVWFVPLYAAEYDLSAGALARLPLPAAGTDEPVGLVLRTDAQPTPVARTLIDAVRDIARARFGDARPRRAARKP
ARRGG

Sequences:

>Translated_325_residues
MNNRIADGRVKFRHLQCFLAVAQLGGVQKAAESLSITQPAVSKTIAELEGILGVKLFERGRHGAQPTREAQLFMPHANAC
VLALRQGVGLLAREGGAAAATLEIGMLPTVAASLAPALMKALAARWPRVVVRIATAANTELLERLKAGAIECAIGRLSEP
ERMVGLAFEQLYNEPLVAVVRAGHPLLASAAPAAELARYPVVLPPYGTLIRQSAEQLLGACGAPPLDAFIEVLSVSVARA
LALENDAVWFVPLYAAEYDLSAGALARLPLPAAGTDEPVGLVLRTDAQPTPVARTLIDAVRDIARARFGDARPRRAARKP
ARRGG
>Mature_325_residues
MNNRIADGRVKFRHLQCFLAVAQLGGVQKAAESLSITQPAVSKTIAELEGILGVKLFERGRHGAQPTREAQLFMPHANAC
VLALRQGVGLLAREGGAAAATLEIGMLPTVAASLAPALMKALAARWPRVVVRIATAANTELLERLKAGAIECAIGRLSEP
ERMVGLAFEQLYNEPLVAVVRAGHPLLASAAPAAELARYPVVLPPYGTLIRQSAEQLLGACGAPPLDAFIEVLSVSVARA
LALENDAVWFVPLYAAEYDLSAGALARLPLPAAGTDEPVGLVLRTDAQPTPVARTLIDAVRDIARARFGDARPRRAARKP
ARRGG

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=299, Percent_Identity=39.1304347826087, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1787601, Length=294, Percent_Identity=26.8707482993197, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790399, Length=277, Percent_Identity=24.5487364620939, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788887, Length=177, Percent_Identity=29.9435028248588, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788297, Length=171, Percent_Identity=29.8245614035088, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=26.0355029585799, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI157672245, Length=206, Percent_Identity=27.1844660194175, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=181, Percent_Identity=29.8342541436464, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34319; Mature: 34319

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRIADGRVKFRHLQCFLAVAQLGGVQKAAESLSITQPAVSKTIAELEGILGVKLFERG
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
RHGAQPTREAQLFMPHANACVLALRQGVGLLAREGGAAAATLEIGMLPTVAASLAPALMK
CCCCCCCCCCEEECCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHH
ALAARWPRVVVRIATAANTELLERLKAGAIECAIGRLSEPERMVGLAFEQLYNEPLVAVV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
RAGHPLLASAAPAAELARYPVVLPPYGTLIRQSAEQLLGACGAPPLDAFIEVLSVSVARA
HCCCCHHHCCCCHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALENDAVWFVPLYAAEYDLSAGALARLPLPAAGTDEPVGLVLRTDAQPTPVARTLIDAV
HHHCCCCEEEEEEEEECCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
RDIARARFGDARPRRAARKPARRGG
HHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MNNRIADGRVKFRHLQCFLAVAQLGGVQKAAESLSITQPAVSKTIAELEGILGVKLFERG
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
RHGAQPTREAQLFMPHANACVLALRQGVGLLAREGGAAAATLEIGMLPTVAASLAPALMK
CCCCCCCCCCEEECCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHH
ALAARWPRVVVRIATAANTELLERLKAGAIECAIGRLSEPERMVGLAFEQLYNEPLVAVV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
RAGHPLLASAAPAAELARYPVVLPPYGTLIRQSAEQLLGACGAPPLDAFIEVLSVSVARA
HCCCCHHHCCCCHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALENDAVWFVPLYAAEYDLSAGALARLPLPAAGTDEPVGLVLRTDAQPTPVARTLIDAV
HHHCCCCEEEEEEEEECCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
RDIARARFGDARPRRAARKPARRGG
HHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]