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The map label for this gene is pgaB [H]

Identifier: 189352795

GI number: 189352795

Start: 909753

End: 911840

Strand: Reverse

Name: pgaB [H]

Synonym: BMULJ_04027

Alternate gene names: 189352795

Gene position: 911840-909753 (Counterclockwise)

Preceding gene: 189352796

Following gene: 189352794

Centisome position: 36.87

GC content: 64.51

Gene sequence:

>2088_bases
ATGCAATCCAGACGGACCTTCATGTGCGGATGCCTCGGGGCATTCGCCGCGTGCTCGTTGTTTCCCGGCGTGACGAAGGC
CAAGATGATCGACCTGCTGCCGCCGTCCGACCCGGCCGACGGCAAGACGTTTCGCGTGATTTGCCTGCACGATGTCCGCG
ACAATCTGCTGTCGACGTTTTCGTCGTCGACGATGGTCGACCCGTTCGCGGTCGACACCGGTACGCTGACCGCGATCTTC
TCGTGGCTCGAGACGAACAACTACCACCCGATCACGGTCAAGCAGATCGAGGCGTCGCGCCACGGCGGGAAGCCGCTGCC
GCCGCGCGCCGTGCTGCTCACGTTCGACGACGGCTACCGCAGCCACTACACGAAGGTGCTGCCGCTGCTCGAACGCTTCC
GCTATCCGGCCGTGATGGGCATCGTCACGTCGTGGATCGATGCGCCGCCCGACGTGCCGATCAAGATCAGCGACAAGGTG
TCGGTGCCGCGCGACTACTTCATGTCGTGGGACGACGTGCGCAAGCTCGGTTCCTCCGATCTGATCGAGCTCGCGTGCCA
CACGCACAATCTGCATCACGGCGCGATCGCGAATCCTCAGGGTAACGAACTGCCGGCCACCACGTCGCACCTGTATCTGC
CTGACGAGAAGCGGTACGAAACCGACGAGGAATTCCACGCACGCGTGCGCAACGACCTGCAGACCTGCATCCGCCAGATG
CGCGAACGCACCGGCATCACCATTCGCTCGATGGTATGGCCGTACGGCGCAGAGAACCAGCCCGTGCGCGAAATCTCGAC
GTCGCTCGGCATGGACATCCAGTTCAGCCTCGACGCAGGCCCGAACACGCCCGATGTGCCGCTCGATCGCCTGCGCCGGA
TTCTGATGATGTACGACGTCGACATCGGCGGCTTCGAGCGGTCGATGCGCGAGCCGGCGACGAACCGCGGCGCCGTCGAT
GCGGTCGAGCGTGTCGTGCAGGTCGATCTCGACCAGGTCTACGACCCGAACCCCGCGCAGCAGGAAAAGAACCTCGGTGT
GCTGATCGAGCGCATCTACCGGATGCAGCCGAAATCGGTCTACCTGCAGGCGTATGCGGATCCGAAAGGCACGGGCGTCG
CCGAGGCCGTGTACTTCCCCAACCGCCATCTACCGATGCGTTCGGACCTGTTCTCGCGCGCCGCGTGGCAGCTCAACACG
CGTTCGAACGTGCAGGTCTATGCGTGGATGCCGGTACTCGCGTTCCGGCCGCCGGCGGACAAGCATCGCGGGCTCGAAGC
GGTCCGCGCGCTCGACGGCGCGCCGGCCCGCGAAAACGGCTCGCGCGTGTTCCGGCTCAGCCCGTTCGATCCCGATGCGC
GGCTGATAATCGAGGAGATCTACGAGGATCTGAGCAAGTACGCGTCGTTCAGCGGCATCCTGTTCAGCGACGATGCGGTG
CTCGACGACTACGAGGACGCAAGCCGCCACGCGCTGCGTGCGTACGCGGAGTGGGGGTTGCCGGCCGACGTCGCGAAGAT
CCGCGGCAATCCCGACCTGATGGCGCGCTGGACGCGGCGCAAGACGCGTCATCTGATCGACTTCACGCTGCAGCTCGAAA
AGATCGTGCGCGCGCACCAGGACGCGGGCGACGTGCTGACCGCGCGCAACATCTTCGCGATGCCGGTGCTGAAGCCCGAG
TCCGAAGCGTGGTACGCGCAGAACTATGCGGACTTCCTCACCACCTACGACTACGTCGCGCTGATGGCGATGCCGTACAT
GGAACAGGCCGACGATCCCGACCGCTGGCTCGACCGGCTCGTGCGCACCGTGCGCGCGAAACCGAACGGGCTGTCGCGCA
CCGTGTTCGAGCTGCAGTCGTACGACTGGCACGCGAACAAGAGCATCGGTGCCGACACGCTGCTTGCGCAAATGCGACGG
CTGCGCAGCGAAGGCGCGGTGAACTTCGGGTACTACCCGGACAACTTCCTCAACAACCAGCCGAATCTCGACACGGTACG
CGACGTGATGTCGCTGAAATCGCGTCTCGATCCGACCTCGATCAATGCACTGATGCAGATGCACCGCAACGGGACCAAGA
CACCATGA

Upstream 100 bases:

>100_bases
CGGCATCGAAGTCAGCAGCCATGCATACGACGGCCAGCGGGAACGCTCGGAAACGGGCTTCCTGTCGGTCAACCTGCCGT
TCTAGTAACAGGAGCTCTCC

Downstream 100 bases:

>100_bases
CCACGCACGGCCTCATTACGAAGCTGCAGGATTTCGTTTTCTACTATCCGTTCTTCATGTCGTACCTGTGGATGATCGGC
GGCGTCGTGCACTACTTCCT

Product: polysaccharide deacetylase family protein

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MQSRRTFMCGCLGAFAACSLFPGVTKAKMIDLLPPSDPADGKTFRVICLHDVRDNLLSTFSSSTMVDPFAVDTGTLTAIF
SWLETNNYHPITVKQIEASRHGGKPLPPRAVLLTFDDGYRSHYTKVLPLLERFRYPAVMGIVTSWIDAPPDVPIKISDKV
SVPRDYFMSWDDVRKLGSSDLIELACHTHNLHHGAIANPQGNELPATTSHLYLPDEKRYETDEEFHARVRNDLQTCIRQM
RERTGITIRSMVWPYGAENQPVREISTSLGMDIQFSLDAGPNTPDVPLDRLRRILMMYDVDIGGFERSMREPATNRGAVD
AVERVVQVDLDQVYDPNPAQQEKNLGVLIERIYRMQPKSVYLQAYADPKGTGVAEAVYFPNRHLPMRSDLFSRAAWQLNT
RSNVQVYAWMPVLAFRPPADKHRGLEAVRALDGAPARENGSRVFRLSPFDPDARLIIEEIYEDLSKYASFSGILFSDDAV
LDDYEDASRHALRAYAEWGLPADVAKIRGNPDLMARWTRRKTRHLIDFTLQLEKIVRAHQDAGDVLTARNIFAMPVLKPE
SEAWYAQNYADFLTTYDYVALMAMPYMEQADDPDRWLDRLVRTVRAKPNGLSRTVFELQSYDWHANKSIGADTLLAQMRR
LRSEGAVNFGYYPDNFLNNQPNLDTVRDVMSLKSRLDPTSINALMQMHRNGTKTP

Sequences:

>Translated_695_residues
MQSRRTFMCGCLGAFAACSLFPGVTKAKMIDLLPPSDPADGKTFRVICLHDVRDNLLSTFSSSTMVDPFAVDTGTLTAIF
SWLETNNYHPITVKQIEASRHGGKPLPPRAVLLTFDDGYRSHYTKVLPLLERFRYPAVMGIVTSWIDAPPDVPIKISDKV
SVPRDYFMSWDDVRKLGSSDLIELACHTHNLHHGAIANPQGNELPATTSHLYLPDEKRYETDEEFHARVRNDLQTCIRQM
RERTGITIRSMVWPYGAENQPVREISTSLGMDIQFSLDAGPNTPDVPLDRLRRILMMYDVDIGGFERSMREPATNRGAVD
AVERVVQVDLDQVYDPNPAQQEKNLGVLIERIYRMQPKSVYLQAYADPKGTGVAEAVYFPNRHLPMRSDLFSRAAWQLNT
RSNVQVYAWMPVLAFRPPADKHRGLEAVRALDGAPARENGSRVFRLSPFDPDARLIIEEIYEDLSKYASFSGILFSDDAV
LDDYEDASRHALRAYAEWGLPADVAKIRGNPDLMARWTRRKTRHLIDFTLQLEKIVRAHQDAGDVLTARNIFAMPVLKPE
SEAWYAQNYADFLTTYDYVALMAMPYMEQADDPDRWLDRLVRTVRAKPNGLSRTVFELQSYDWHANKSIGADTLLAQMRR
LRSEGAVNFGYYPDNFLNNQPNLDTVRDVMSLKSRLDPTSINALMQMHRNGTKTP
>Mature_695_residues
MQSRRTFMCGCLGAFAACSLFPGVTKAKMIDLLPPSDPADGKTFRVICLHDVRDNLLSTFSSSTMVDPFAVDTGTLTAIF
SWLETNNYHPITVKQIEASRHGGKPLPPRAVLLTFDDGYRSHYTKVLPLLERFRYPAVMGIVTSWIDAPPDVPIKISDKV
SVPRDYFMSWDDVRKLGSSDLIELACHTHNLHHGAIANPQGNELPATTSHLYLPDEKRYETDEEFHARVRNDLQTCIRQM
RERTGITIRSMVWPYGAENQPVREISTSLGMDIQFSLDAGPNTPDVPLDRLRRILMMYDVDIGGFERSMREPATNRGAVD
AVERVVQVDLDQVYDPNPAQQEKNLGVLIERIYRMQPKSVYLQAYADPKGTGVAEAVYFPNRHLPMRSDLFSRAAWQLNT
RSNVQVYAWMPVLAFRPPADKHRGLEAVRALDGAPARENGSRVFRLSPFDPDARLIIEEIYEDLSKYASFSGILFSDDAV
LDDYEDASRHALRAYAEWGLPADVAKIRGNPDLMARWTRRKTRHLIDFTLQLEKIVRAHQDAGDVLTARNIFAMPVLKPE
SEAWYAQNYADFLTTYDYVALMAMPYMEQADDPDRWLDRLVRTVRAKPNGLSRTVFELQSYDWHANKSIGADTLLAQMRR
LRSEGAVNFGYYPDNFLNNQPNLDTVRDVMSLKSRLDPTSINALMQMHRNGTKTP

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=654, Percent_Identity=41.5902140672783, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 78944; Mature: 78944

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRRTFMCGCLGAFAACSLFPGVTKAKMIDLLPPSDPADGKTFRVICLHDVRDNLLSTF
CCCCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
SSSTMVDPFAVDTGTLTAIFSWLETNNYHPITVKQIEASRHGGKPLPPRAVLLTFDDGYR
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCEEEEEECCCHH
SHYTKVLPLLERFRYPAVMGIVTSWIDAPPDVPIKISDKVSVPRDYFMSWDDVRKLGSSD
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHHHHHCCCCH
LIELACHTHNLHHGAIANPQGNELPATTSHLYLPDEKRYETDEEFHARVRNDLQTCIRQM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RERTGITIRSMVWPYGAENQPVREISTSLGMDIQFSLDAGPNTPDVPLDRLRRILMMYDV
HHHHCCEEEHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
DIGGFERSMREPATNRGAVDAVERVVQVDLDQVYDPNPAQQEKNLGVLIERIYRMQPKSV
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCEE
YLQAYADPKGTGVAEAVYFPNRHLPMRSDLFSRAAWQLNTRSNVQVYAWMPVLAFRPPAD
EEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCEEEEEEECEEEECCCCH
KHRGLEAVRALDGAPARENGSRVFRLSPFDPDARLIIEEIYEDLSKYASFSGILFSDDAV
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
LDDYEDASRHALRAYAEWGLPADVAKIRGNPDLMARWTRRKTRHLIDFTLQLEKIVRAHQ
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAGDVLTARNIFAMPVLKPESEAWYAQNYADFLTTYDYVALMAMPYMEQADDPDRWLDRL
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VRTVRAKPNGLSRTVFELQSYDWHANKSIGADTLLAQMRRLRSEGAVNFGYYPDNFLNNQ
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHCCCC
PNLDTVRDVMSLKSRLDPTSINALMQMHRNGTKTP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQSRRTFMCGCLGAFAACSLFPGVTKAKMIDLLPPSDPADGKTFRVICLHDVRDNLLSTF
CCCCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
SSSTMVDPFAVDTGTLTAIFSWLETNNYHPITVKQIEASRHGGKPLPPRAVLLTFDDGYR
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCEEEEEECCCHH
SHYTKVLPLLERFRYPAVMGIVTSWIDAPPDVPIKISDKVSVPRDYFMSWDDVRKLGSSD
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHHHHHCCCCH
LIELACHTHNLHHGAIANPQGNELPATTSHLYLPDEKRYETDEEFHARVRNDLQTCIRQM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RERTGITIRSMVWPYGAENQPVREISTSLGMDIQFSLDAGPNTPDVPLDRLRRILMMYDV
HHHHCCEEEHHCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
DIGGFERSMREPATNRGAVDAVERVVQVDLDQVYDPNPAQQEKNLGVLIERIYRMQPKSV
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCEE
YLQAYADPKGTGVAEAVYFPNRHLPMRSDLFSRAAWQLNTRSNVQVYAWMPVLAFRPPAD
EEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCEEEEEEECEEEECCCCH
KHRGLEAVRALDGAPARENGSRVFRLSPFDPDARLIIEEIYEDLSKYASFSGILFSDDAV
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
LDDYEDASRHALRAYAEWGLPADVAKIRGNPDLMARWTRRKTRHLIDFTLQLEKIVRAHQ
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAGDVLTARNIFAMPVLKPESEAWYAQNYADFLTTYDYVALMAMPYMEQADDPDRWLDRL
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VRTVRAKPNGLSRTVFELQSYDWHANKSIGADTLLAQMRRLRSEGAVNFGYYPDNFLNNQ
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHCCCC
PNLDTVRDVMSLKSRLDPTSINALMQMHRNGTKTP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]