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The map label for this gene is pgaC [H]

Identifier: 189352794

GI number: 189352794

Start: 908485

End: 909756

Strand: Reverse

Name: pgaC [H]

Synonym: BMULJ_04026

Alternate gene names: 189352794

Gene position: 909756-908485 (Counterclockwise)

Preceding gene: 189352795

Following gene: 189352793

Centisome position: 36.79

GC content: 62.26

Gene sequence:

>1272_bases
ATGACCACGCACGGCCTCATTACGAAGCTGCAGGATTTCGTTTTCTACTATCCGTTCTTCATGTCGTACCTGTGGATGAT
CGGCGGCGTCGTGCACTACTTCCTGCTCGAAGAGGGTCGCGTGCTGTCGAAGCGCAAGATCGCCGCGAGCGGCTTCCCGA
AGGTGTCGATCGTCGTGCCGTGCTTCAACGAGGAGGCGAACGCACGCAGCGTGATCGAGCATCTGAACGCGATGGACTAT
CCGAACTACGACATCATCGCGGTCAACGACGGCAGCAAGGACCGCACCGGCGAAATCCTGAACCAGCTCGCGGTGGAGAT
CCCGCGGCTGCTCGTGATTCATCACGCGCGCAACGAAGGCAAGGCGGTCGGGCTCACGACCGCGGCCGCGATGTCGAACG
CCGAGTATCTGCTCTGCATCGACGGCGACTCGCTGCTCGGCCACGACGCGATCGAATGGATGCTCGACCATTTCCTGACC
GATCCGGGCGTCGGCGCGGTGACCGGCAATCCGCGCATCCGCACGCGCACGTCGCTGCTCGGCCGCATGCAGGTCGGCGA
GTTCTCGTCGATCGTCGGGCTGATCAAGCGCACGCAGCAGGTGTACGGCCGCATCTTCACGGTATCGGGCGTGATCACGA
TGTTCCGCAAGACCGCGCTCGCCGACGTCGGATTCTGGAGCACCGACATGCTGACCGAGGACATCGACATCAGCTGGAAG
CTGCAGTGCAGCGACTGGCGCGTCGTGTACGAACCGCATGCGCTGAGCTGGATCCTGATGCCGGAAACCGTGAAGGGCCT
GTACCGGCAGCGCCTGCGCTGGGCGAAAGGCGGCATCCAGGTGCTGATGAAGTATGCCGGCGCGCTCGCGCGGCCGACGC
GGATGATGATGTGGCCGCTGTTCATCGAGTATCTGATCGGCATCGCCTGGGCCTACGCGATGTCGTTCATTCTGCTGCTC
GCGCTCGTGGATGTCATCTATCCGCTGCCGCCGAGCTGGCATGTTTCCGTCGTACCGCATTGGCACGGGATGCTGCTGGT
CGCCACCTGCATCCTGCAGCTGATCATCGGCAGCATGATCGATCGTCACTACGACGAGAAGCTGCTGATGTATTTCCTTG
ACACCGTCTGGTACCCCGTCGCTTTCTGGCTGATCAGCATGATCACTACCGTCGTCGCGCTGCCGGCGGTCGTGCTGCGA
GGCCGCGGCAAGCGAGCCGTGTGGGTCAGTCCCGACCGAGGCATTCAACATGAACAACGCACCGATTATTGA

Upstream 100 bases:

>100_bases
ATCTCGACACGGTACGCGACGTGATGTCGCTGAAATCGCGTCTCGATCCGACCTCGATCAATGCACTGATGCAGATGCAC
CGCAACGGGACCAAGACACC

Downstream 100 bases:

>100_bases
CCTCTCCCTGCGCACTCCGCGCGAAATGATCGCCGAACGAGGCGGCGTCGTCGGCCCGCTGCTGGTCGCCTGGTTCCGCT
TCGGCCGACCCGCGCTGGTC

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTTHGLITKLQDFVFYYPFFMSYLWMIGGVVHYFLLEEGRVLSKRKIAASGFPKVSIVVPCFNEEANARSVIEHLNAMDY
PNYDIIAVNDGSKDRTGEILNQLAVEIPRLLVIHHARNEGKAVGLTTAAAMSNAEYLLCIDGDSLLGHDAIEWMLDHFLT
DPGVGAVTGNPRIRTRTSLLGRMQVGEFSSIVGLIKRTQQVYGRIFTVSGVITMFRKTALADVGFWSTDMLTEDIDISWK
LQCSDWRVVYEPHALSWILMPETVKGLYRQRLRWAKGGIQVLMKYAGALARPTRMMMWPLFIEYLIGIAWAYAMSFILLL
ALVDVIYPLPPSWHVSVVPHWHGMLLVATCILQLIIGSMIDRHYDEKLLMYFLDTVWYPVAFWLISMITTVVALPAVVLR
GRGKRAVWVSPDRGIQHEQRTDY

Sequences:

>Translated_423_residues
MTTHGLITKLQDFVFYYPFFMSYLWMIGGVVHYFLLEEGRVLSKRKIAASGFPKVSIVVPCFNEEANARSVIEHLNAMDY
PNYDIIAVNDGSKDRTGEILNQLAVEIPRLLVIHHARNEGKAVGLTTAAAMSNAEYLLCIDGDSLLGHDAIEWMLDHFLT
DPGVGAVTGNPRIRTRTSLLGRMQVGEFSSIVGLIKRTQQVYGRIFTVSGVITMFRKTALADVGFWSTDMLTEDIDISWK
LQCSDWRVVYEPHALSWILMPETVKGLYRQRLRWAKGGIQVLMKYAGALARPTRMMMWPLFIEYLIGIAWAYAMSFILLL
ALVDVIYPLPPSWHVSVVPHWHGMLLVATCILQLIIGSMIDRHYDEKLLMYFLDTVWYPVAFWLISMITTVVALPAVVLR
GRGKRAVWVSPDRGIQHEQRTDY
>Mature_422_residues
TTHGLITKLQDFVFYYPFFMSYLWMIGGVVHYFLLEEGRVLSKRKIAASGFPKVSIVVPCFNEEANARSVIEHLNAMDYP
NYDIIAVNDGSKDRTGEILNQLAVEIPRLLVIHHARNEGKAVGLTTAAAMSNAEYLLCIDGDSLLGHDAIEWMLDHFLTD
PGVGAVTGNPRIRTRTSLLGRMQVGEFSSIVGLIKRTQQVYGRIFTVSGVITMFRKTALADVGFWSTDMLTEDIDISWKL
QCSDWRVVYEPHALSWILMPETVKGLYRQRLRWAKGGIQVLMKYAGALARPTRMMMWPLFIEYLIGIAWAYAMSFILLLA
LVDVIYPLPPSWHVSVVPHWHGMLLVATCILQLIIGSMIDRHYDEKLLMYFLDTVWYPVAFWLISMITTVVALPAVVLRG
RGKRAVWVSPDRGIQHEQRTDY

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=409, Percent_Identity=49.3887530562347, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48077; Mature: 47946

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHGLITKLQDFVFYYPFFMSYLWMIGGVVHYFLLEEGRVLSKRKIAASGFPKVSIVVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
CFNEEANARSVIEHLNAMDYPNYDIIAVNDGSKDRTGEILNQLAVEIPRLLVIHHARNEG
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCCC
KAVGLTTAAAMSNAEYLLCIDGDSLLGHDAIEWMLDHFLTDPGVGAVTGNPRIRTRTSLL
CEEEEEHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHH
GRMQVGEFSSIVGLIKRTQQVYGRIFTVSGVITMFRKTALADVGFWSTDMLTEDIDISWK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEE
LQCSDWRVVYEPHALSWILMPETVKGLYRQRLRWAKGGIQVLMKYAGALARPTRMMMWPL
EEECCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FIEYLIGIAWAYAMSFILLLALVDVIYPLPPSWHVSVVPHWHGMLLVATCILQLIIGSMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
DRHYDEKLLMYFLDTVWYPVAFWLISMITTVVALPAVVLRGRGKRAVWVSPDRGIQHEQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
TDY
CCC
>Mature Secondary Structure 
TTHGLITKLQDFVFYYPFFMSYLWMIGGVVHYFLLEEGRVLSKRKIAASGFPKVSIVVP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
CFNEEANARSVIEHLNAMDYPNYDIIAVNDGSKDRTGEILNQLAVEIPRLLVIHHARNEG
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCCC
KAVGLTTAAAMSNAEYLLCIDGDSLLGHDAIEWMLDHFLTDPGVGAVTGNPRIRTRTSLL
CEEEEEHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHH
GRMQVGEFSSIVGLIKRTQQVYGRIFTVSGVITMFRKTALADVGFWSTDMLTEDIDISWK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEE
LQCSDWRVVYEPHALSWILMPETVKGLYRQRLRWAKGGIQVLMKYAGALARPTRMMMWPL
EEECCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FIEYLIGIAWAYAMSFILLLALVDVIYPLPPSWHVSVVPHWHGMLLVATCILQLIIGSMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
DRHYDEKLLMYFLDTVWYPVAFWLISMITTVVALPAVVLRGRGKRAVWVSPDRGIQHEQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
TDY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]