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The map label for this gene is pgaA [H]

Identifier: 189352796

GI number: 189352796

Start: 911856

End: 914249

Strand: Reverse

Name: pgaA [H]

Synonym: BMULJ_04028

Alternate gene names: 189352796

Gene position: 914249-911856 (Counterclockwise)

Preceding gene: 189352797

Following gene: 189352795

Centisome position: 36.97

GC content: 69.05

Gene sequence:

>2394_bases
GTGGCCAACAATCTGAAACAACAATGCACCACCGTCGCTCCGCCGAACCGGCGGATGCTCGTGCACGCGCCGCTCGTCTT
GCTGCTCGTCGCCTCCGGCTCCTGTTTCGCCGCGGACAGCGATGCGGACGCCGTCGGCGCAAGCGGCACCGCCGGCACGC
AGGGCGCGTCCGAAACCATGCCGGCCGACGTGCCGACCGCCGGCGCAACGGCGCAGGACGCGACGGCGCCCGATACAACG
GCGCCTGACGCGACCGCACCCGGCGCGACGGCGCCCGGCACATCGGCGCCCCACGCAACGCCACCATCGGCACAGCCGCC
TGCCGCACCGACGGCGATCGCGCAAGTGTCGGGCACGGCGATGCCGACCGCACCGAGCCCAACACCGGCCGGCGCGGCGC
CGATGATCGGCGCGCCGTCGATCGAGCGCCCCGACGTGACACCGCCGCCGCAGCAGCAGCCGGAGGCGCAGGCGCCGAAC
GCCCAGGCACCGAATCTGCGCACCGCCGACCCCACGACCGTCCGCAACGACGTGTTCGGGCTCGCGGTCAGCGGCGGCGC
CGTCAAGGCGCTCGACGAAGCGAAGGCGCGGCCCGATGCGTTCTCGCCGGTCGATCTCGCGCAACTCGAGGAACTGTCGA
TCCGTCAGCAGGTACGCGGCGGCCGCGACAAGGCGCGCTCGATGACGAGCGCCGACCGCTTCGACGGCATCGACCAGGCA
CTGAAGGCCGCCGACGATCTCGACTCGCGCATGCCGGTCACACCCGAGTATTCGTCGGTGCGCACGGCGCTCGCGGGCGA
CCGCACGATCGCCTACGCAGCGCGCGGCGACATGAACAAGGCGATCGCAACGTTCGAGACGATTCCGCCCGACGCGGACA
TCTCGATCGACGCGCTGGCCGCGGTGGGCGACGCGTATCTCTACGTCAGCGAACCGGGCAAGGCGAGCGCCGTCTACGAG
CGCGCGCTGAAACAGGCGAGCGCGCCGGCGGGCGACCGCGCGACGCGCGGCTTCCAGTACGGTTCGCGCACGCGGCCGAT
CGAGCTGCGCGAGGGACTGTTCTGGTCGTATATCGATCAGGCACGCGCCGCCGATGCGGGCCGCGTGCTGAACGACATCG
ACACGGCCTTGCCCCCGGCGAGCCAGGTACATCCGTGGGATCCGGCAAACGAGGATTACCTGCGTTATTACCGGCTGCGC
GCGCAGTACCTGATCTACTCGGGCCGTGTCGACGAAGGCATCGCGGAGCTCGAGCGGCTCGAAAAGGAGGTGCCGTTCAA
TGCCGAGGTGCGCTCCGCGCATGCCGACGCGGTGTCGGGGCAGTCGCATCCGCGTCAGGCGATGGCGATGTACAACGCGA
CGCTGACCGATCACCCGGACAACGTCGAAATGCGTGCGGGCCTCGGCCGCGCAGCGTTGACGGCGGACGACTACGCGACC
GCGCGCAACGTCGACGAAACGCTCGACAACCTGTTTCCGGACAGCGCCGCGGTGCGCTCGTTCAAGCGCGACTACAACGC
CTACCGCAGCCCGGTGTTTACGACCGACATCAGCTACGAACACGGCAACAGCGCGCTGGCCGACAACAGCTTCTCGTCGG
ACAGCTATATCTATTCGCAGCCGTTCGGCGACAACTGGCGCGTGTTCTCGCACACGTTCTACGGCTTCGCGCAGACCGAC
ACGGGCCGCATCAGCCGCACGCGCACGGGCGTCGGGGGCGACTACCGGCACGGGCCGCTGACGGTCCAGGCGGAAGCGAC
GCGCTCGTTCGGCGGCGACGGCCGCACCGGCGGACGCGGCACCGTCACCTTCAACGTCGACGACTACTGGACGCTGAGCG
GCGCGGTCGACAGCAACGACAACTCGCTGCCGTGGAAAGCGTACGTCGCGCACATCTGGGGCCGTTCGGCCAACGTCGAG
ATCGCGTACCGGCAGAACGACCGGCGCGAGGTGAAGCTCACCTACGGCGTGAGCCGCTTCAGCGATTCGAACTTCCACCA
GGAAATCGCCGCGACCGGCACGCAGCGCGTGTACACGTCCGCGAACCAGCTCGTCAACGTGTCGCTCGGCCTCGGCACCG
ACAGCAACACGCGCCAGGACACGCCCTACTTCAGTCCGAGACGCGACTACGCGGCGGCCGTCACCGCGATGCATCAAATG
ACGCTGTGGAAGAAAGGCGACATGTCGCTGCAGCAGCGCCTGTACGCGTCGGGCGGCGCGTTCAACCAGCGCGGCTTCGG
CACGAGCGCGCTGTGGAGCGCGCGCCTCGAGCACGCGTGGACGTTCAAGCGCGACATCACGCTCAGCTACGGCATCGAAG
TCAGCAGCCATGCATACGACGGCCAGCGGGAACGCTCGGAAACGGGCTTCCTGTCGGTCAACCTGCCGTTCTAG

Upstream 100 bases:

>100_bases
ACCTAGCGCAGCCGGGGCCCTAAACCCGGCCGCACGGCAACCGGCGACTCGTGTCGCCGGGTATGCGATCGACGTGATCG
TCTCGAAGTGAGGACACACC

Downstream 100 bases:

>100_bases
TAACAGGAGCTCTCCATGCAATCCAGACGGACCTTCATGTGCGGATGCCTCGGGGCATTCGCCGCGTGCTCGTTGTTTCC
CGGCGTGACGAAGGCCAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MANNLKQQCTTVAPPNRRMLVHAPLVLLLVASGSCFAADSDADAVGASGTAGTQGASETMPADVPTAGATAQDATAPDTT
APDATAPGATAPGTSAPHATPPSAQPPAAPTAIAQVSGTAMPTAPSPTPAGAAPMIGAPSIERPDVTPPPQQQPEAQAPN
AQAPNLRTADPTTVRNDVFGLAVSGGAVKALDEAKARPDAFSPVDLAQLEELSIRQQVRGGRDKARSMTSADRFDGIDQA
LKAADDLDSRMPVTPEYSSVRTALAGDRTIAYAARGDMNKAIATFETIPPDADISIDALAAVGDAYLYVSEPGKASAVYE
RALKQASAPAGDRATRGFQYGSRTRPIELREGLFWSYIDQARAADAGRVLNDIDTALPPASQVHPWDPANEDYLRYYRLR
AQYLIYSGRVDEGIAELERLEKEVPFNAEVRSAHADAVSGQSHPRQAMAMYNATLTDHPDNVEMRAGLGRAALTADDYAT
ARNVDETLDNLFPDSAAVRSFKRDYNAYRSPVFTTDISYEHGNSALADNSFSSDSYIYSQPFGDNWRVFSHTFYGFAQTD
TGRISRTRTGVGGDYRHGPLTVQAEATRSFGGDGRTGGRGTVTFNVDDYWTLSGAVDSNDNSLPWKAYVAHIWGRSANVE
IAYRQNDRREVKLTYGVSRFSDSNFHQEIAATGTQRVYTSANQLVNVSLGLGTDSNTRQDTPYFSPRRDYAAAVTAMHQM
TLWKKGDMSLQQRLYASGGAFNQRGFGTSALWSARLEHAWTFKRDITLSYGIEVSSHAYDGQRERSETGFLSVNLPF

Sequences:

>Translated_797_residues
MANNLKQQCTTVAPPNRRMLVHAPLVLLLVASGSCFAADSDADAVGASGTAGTQGASETMPADVPTAGATAQDATAPDTT
APDATAPGATAPGTSAPHATPPSAQPPAAPTAIAQVSGTAMPTAPSPTPAGAAPMIGAPSIERPDVTPPPQQQPEAQAPN
AQAPNLRTADPTTVRNDVFGLAVSGGAVKALDEAKARPDAFSPVDLAQLEELSIRQQVRGGRDKARSMTSADRFDGIDQA
LKAADDLDSRMPVTPEYSSVRTALAGDRTIAYAARGDMNKAIATFETIPPDADISIDALAAVGDAYLYVSEPGKASAVYE
RALKQASAPAGDRATRGFQYGSRTRPIELREGLFWSYIDQARAADAGRVLNDIDTALPPASQVHPWDPANEDYLRYYRLR
AQYLIYSGRVDEGIAELERLEKEVPFNAEVRSAHADAVSGQSHPRQAMAMYNATLTDHPDNVEMRAGLGRAALTADDYAT
ARNVDETLDNLFPDSAAVRSFKRDYNAYRSPVFTTDISYEHGNSALADNSFSSDSYIYSQPFGDNWRVFSHTFYGFAQTD
TGRISRTRTGVGGDYRHGPLTVQAEATRSFGGDGRTGGRGTVTFNVDDYWTLSGAVDSNDNSLPWKAYVAHIWGRSANVE
IAYRQNDRREVKLTYGVSRFSDSNFHQEIAATGTQRVYTSANQLVNVSLGLGTDSNTRQDTPYFSPRRDYAAAVTAMHQM
TLWKKGDMSLQQRLYASGGAFNQRGFGTSALWSARLEHAWTFKRDITLSYGIEVSSHAYDGQRERSETGFLSVNLPF
>Mature_796_residues
ANNLKQQCTTVAPPNRRMLVHAPLVLLLVASGSCFAADSDADAVGASGTAGTQGASETMPADVPTAGATAQDATAPDTTA
PDATAPGATAPGTSAPHATPPSAQPPAAPTAIAQVSGTAMPTAPSPTPAGAAPMIGAPSIERPDVTPPPQQQPEAQAPNA
QAPNLRTADPTTVRNDVFGLAVSGGAVKALDEAKARPDAFSPVDLAQLEELSIRQQVRGGRDKARSMTSADRFDGIDQAL
KAADDLDSRMPVTPEYSSVRTALAGDRTIAYAARGDMNKAIATFETIPPDADISIDALAAVGDAYLYVSEPGKASAVYER
ALKQASAPAGDRATRGFQYGSRTRPIELREGLFWSYIDQARAADAGRVLNDIDTALPPASQVHPWDPANEDYLRYYRLRA
QYLIYSGRVDEGIAELERLEKEVPFNAEVRSAHADAVSGQSHPRQAMAMYNATLTDHPDNVEMRAGLGRAALTADDYATA
RNVDETLDNLFPDSAAVRSFKRDYNAYRSPVFTTDISYEHGNSALADNSFSSDSYIYSQPFGDNWRVFSHTFYGFAQTDT
GRISRTRTGVGGDYRHGPLTVQAEATRSFGGDGRTGGRGTVTFNVDDYWTLSGAVDSNDNSLPWKAYVAHIWGRSANVEI
AYRQNDRREVKLTYGVSRFSDSNFHQEIAATGTQRVYTSANQLVNVSLGLGTDSNTRQDTPYFSPRRDYAAAVTAMHQMT
LWKKGDMSLQQRLYASGGAFNQRGFGTSALWSARLEHAWTFKRDITLSYGIEVSSHAYDGQRERSETGFLSVNLPF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=578, Percent_Identity=22.6643598615917, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 85617; Mature: 85486

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNLKQQCTTVAPPNRRMLVHAPLVLLLVASGSCFAADSDADAVGASGTAGTQGASETM
CCCCHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
PADVPTAGATAQDATAPDTTAPDATAPGATAPGTSAPHATPPSAQPPAAPTAIAQVSGTA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
MPTAPSPTPAGAAPMIGAPSIERPDVTPPPQQQPEAQAPNAQAPNLRTADPTTVRNDVFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEE
LAVSGGAVKALDEAKARPDAFSPVDLAQLEELSIRQQVRGGRDKARSMTSADRFDGIDQA
EEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
LKAADDLDSRMPVTPEYSSVRTALAGDRTIAYAARGDMNKAIATFETIPPDADISIDALA
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHH
AVGDAYLYVSEPGKASAVYERALKQASAPAGDRATRGFQYGSRTRPIELREGLFWSYIDQ
HCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHCCHHHHHHHH
ARAADAGRVLNDIDTALPPASQVHPWDPANEDYLRYYRLRAQYLIYSGRVDEGIAELERL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHHHH
EKEVPFNAEVRSAHADAVSGQSHPRQAMAMYNATLTDHPDNVEMRAGLGRAALTADDYAT
HHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCEECCCCCCCHHHCCCCCCEECCCHHHH
ARNVDETLDNLFPDSAAVRSFKRDYNAYRSPVFTTDISYEHGNSALADNSFSSDSYIYSQ
HCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEC
PFGDNWRVFSHTFYGFAQTDTGRISRTRTGVGGDYRHGPLTVQAEATRSFGGDGRTGGRG
CCCCCCEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCE
TVTFNVDDYWTLSGAVDSNDNSLPWKAYVAHIWGRSANVEIAYRQNDRREVKLTYGVSRF
EEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHC
SDSNFHQEIAATGTQRVYTSANQLVNVSLGLGTDSNTRQDTPYFSPRRDYAAAVTAMHQM
CCCCHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLWKKGDMSLQQRLYASGGAFNQRGFGTSALWSARLEHAWTFKRDITLSYGIEVSSHAYD
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEECEEEEECCEECCCCCC
GQRERSETGFLSVNLPF
CCHHCCCCCEEEEECCC
>Mature Secondary Structure 
ANNLKQQCTTVAPPNRRMLVHAPLVLLLVASGSCFAADSDADAVGASGTAGTQGASETM
CCCHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
PADVPTAGATAQDATAPDTTAPDATAPGATAPGTSAPHATPPSAQPPAAPTAIAQVSGTA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
MPTAPSPTPAGAAPMIGAPSIERPDVTPPPQQQPEAQAPNAQAPNLRTADPTTVRNDVFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEE
LAVSGGAVKALDEAKARPDAFSPVDLAQLEELSIRQQVRGGRDKARSMTSADRFDGIDQA
EEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
LKAADDLDSRMPVTPEYSSVRTALAGDRTIAYAARGDMNKAIATFETIPPDADISIDALA
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHH
AVGDAYLYVSEPGKASAVYERALKQASAPAGDRATRGFQYGSRTRPIELREGLFWSYIDQ
HCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHCCHHHHHHHH
ARAADAGRVLNDIDTALPPASQVHPWDPANEDYLRYYRLRAQYLIYSGRVDEGIAELERL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHHHH
EKEVPFNAEVRSAHADAVSGQSHPRQAMAMYNATLTDHPDNVEMRAGLGRAALTADDYAT
HHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCEECCCCCCCHHHCCCCCCEECCCHHHH
ARNVDETLDNLFPDSAAVRSFKRDYNAYRSPVFTTDISYEHGNSALADNSFSSDSYIYSQ
HCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEC
PFGDNWRVFSHTFYGFAQTDTGRISRTRTGVGGDYRHGPLTVQAEATRSFGGDGRTGGRG
CCCCCCEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCE
TVTFNVDDYWTLSGAVDSNDNSLPWKAYVAHIWGRSANVEIAYRQNDRREVKLTYGVSRF
EEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHC
SDSNFHQEIAATGTQRVYTSANQLVNVSLGLGTDSNTRQDTPYFSPRRDYAAAVTAMHQM
CCCCHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLWKKGDMSLQQRLYASGGAFNQRGFGTSALWSARLEHAWTFKRDITLSYGIEVSSHAYD
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEECEEEEECCEECCCCCC
GQRERSETGFLSVNLPF
CCHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]