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The map label for this gene is pcaJ [H]

Identifier: 189352017

GI number: 189352017

Start: 35709

End: 36362

Strand: Direct

Name: pcaJ [H]

Synonym: BMULJ_03234

Alternate gene names: 189352017

Gene position: 35709-36362 (Clockwise)

Preceding gene: 189352016

Following gene: 189352018

Centisome position: 1.44

GC content: 66.06

Gene sequence:

>654_bases
ATGAAACGACTGACCCGCGATGAAATGGCCAAGCGCGTCGCCCAGGACATCCCCGAAGGCGCGTATGTGAACCTCGGCAT
CGGCGTGCCGACGCTGGTGGCGAACCACCTCGATCCGAGCAAGGAAATCTTCCTGCACAGCGAGAACGGCCTGCTCGGCA
TGGGCCCGGCGCCCGCCCCCGGCGAGGAAGACGACGAGCTGATCAACGCCGGCAAGCAGCACGTGACGCTGCTCACGGGC
GGCGCCTACTTCCACCATGCCGATTCGTTCGCGATGATGCGCGGCGGCCACCTCGACTACTGCGTGCTCGGCGCGTTTCA
GGTGTCCGCGAACGGCGACCTCGCGAACTGGCATACGGGTGCGCCCGATGCGATTCCGGCGGTCGGCGGCGCGATGGATC
TCGCGATCGGCGCGAAGCAGGTGTTCGTGATGATGGAGCACCTGACGAAGCAGGGCGAGAGCAAGATCGTCGCGCAGTGC
TCGTATCCGGTGACGGGCGTGCAGTGCGTGAGCCGCATCTATACCGATCTCGCCGTGCTCGACGTGACGTCCGACGGCCT
CGCGGTGAGCGAGATCTTCACCGACCTGTCGTTCGACGAACTGCAGAAGCTGACCGGCGTGCCGCTGATCGACGCGACGC
GCAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TTCGTGCAGCGCATCGTCGAAGTGCCGCAGGCCGCGCATGCGGCCGAGCTGGCCGCAGAACGCGCATCGCAAGCCGCCTG
ACCGCCCGAGGAGATCCGAC

Downstream 100 bases:

>100_bases
ACCGCAAGCGGCCGGCGCGCGCGTGCGCACGCCGGCGCGCTTCGGTGGACAATAGGCCACCCGTTTTCCCATCCGACGCC
ATGCTCGAAGACAGCGCCCG

Product: 3-oxoadipate CoA-transferase beta subunit

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKRLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLDPSKEIFLHSENGLLGMGPAPAPGEEDDELINAGKQHVTLLTG
GAYFHHADSFAMMRGGHLDYCVLGAFQVSANGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAQC
SYPVTGVQCVSRIYTDLAVLDVTSDGLAVSEIFTDLSFDELQKLTGVPLIDATRKAA

Sequences:

>Translated_217_residues
MKRLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLDPSKEIFLHSENGLLGMGPAPAPGEEDDELINAGKQHVTLLTG
GAYFHHADSFAMMRGGHLDYCVLGAFQVSANGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAQC
SYPVTGVQCVSRIYTDLAVLDVTSDGLAVSEIFTDLSFDELQKLTGVPLIDATRKAA
>Mature_217_residues
MKRLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLDPSKEIFLHSENGLLGMGPAPAPGEEDDELINAGKQHVTLLTG
GAYFHHADSFAMMRGGHLDYCVLGAFQVSANGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAQC
SYPVTGVQCVSRIYTDLAVLDVTSDGLAVSEIFTDLSFDELQKLTGVPLIDATRKAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=48.2758620689655, Blast_Score=189, Evalue=1e-48,
Organism=Homo sapiens, GI11545841, Length=203, Percent_Identity=47.7832512315271, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1788552, Length=187, Percent_Identity=43.8502673796791, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531517, Length=204, Percent_Identity=48.5294117647059, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21358325, Length=206, Percent_Identity=42.7184466019417, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24655794, Length=206, Percent_Identity=42.7184466019417, Blast_Score=167, Evalue=4e-42,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23062; Mature: 23062

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLDPSKEIFLHSENGLLGMGPAPAP
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCC
GEEDDELINAGKQHVTLLTGGAYFHHADSFAMMRGGHLDYCVLGAFQVSANGDLANWHTG
CCCCHHHHHCCCCEEEEEECCEEEECCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAQCSYPVTGVQCVSRIYTDLAVL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHEEE
DVTSDGLAVSEIFTDLSFDELQKLTGVPLIDATRKAA
EECCCCEEHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRLTRDEMAKRVAQDIPEGAYVNLGIGVPTLVANHLDPSKEIFLHSENGLLGMGPAPAP
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCC
GEEDDELINAGKQHVTLLTGGAYFHHADSFAMMRGGHLDYCVLGAFQVSANGDLANWHTG
CCCCHHHHHCCCCEEEEEECCEEEECCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAQCSYPVTGVQCVSRIYTDLAVL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHEEE
DVTSDGLAVSEIFTDLSFDELQKLTGVPLIDATRKAA
EECCCCEEHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]