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The map label for this gene is purB [H]

Identifier: 189352018

GI number: 189352018

Start: 36443

End: 37798

Strand: Direct

Name: purB [H]

Synonym: BMULJ_03235

Alternate gene names: 189352018

Gene position: 36443-37798 (Clockwise)

Preceding gene: 189352017

Following gene: 189352019

Centisome position: 1.47

GC content: 71.76

Gene sequence:

>1356_bases
ATGCTCGAAGACAGCGCCCGCCTGACCTCCCTCATCTGCGGCATCGAGCCGCTCAACCGGATCTGGTCGCCCCGTGCCAC
CGTCCAGCGGATGCTCGACGTCGAAGCCGCGCTCGCGCGCGCGCTGGCCGCGCAACAGGTCATCCCGGCCGCCGCGGTCG
CGCCGATCGAACGCGCGTGCGTGGCCGACCGGCTCGATGCCGATGCGCTCGCGCGCGATGCGGCGCTCGGCGGCAATCTC
GCGATTCCGCTCGTCAAGCAGCTCACCGCGCAGGTGAAGGCCGACGATGCCGAGGCCGCGAAATTCGTGCACTGGGGCGC
GACGAGCCAGGACATCATCGATACCGCGACCGTCCTTCAGCTGCGCGATACGCTCGACGTGCTCGAACCGATGCTCGACG
ACGCGTGCGCGTCGCTCGCGACGCTCGCGCGCACACATCGCGCGACGCCGATGATCGGCCGCACGTGGCTGCAGCAGGCA
TTGCCGATCACGCTCGGGCTGAAGTTCGCGCAGTGGCTCGACGCGCTGCTGCGTCATCGCACGCGGCTCGCCGAGCTGCG
CGCGCGTGCGCTCGTGCTGCAGTTCGGCGGCGCGGCCGGCACGCTCGCGAGCCTGCGCGATCGCGCGAGCGGCGTGAGCG
CCGCACTGGCCAAAGAGCTCGAGCTCGCCGCACCGGCGGTGCCGTGGCATACGCAGCGCGACCGCATCGCCGAGGCCGCG
GCATGGTTCGGTATGCTGATCGGTACGCTCGGCAAGATCGCGCGCGACATCTCGCTGCAGATGCAGACCGAAGTCGGCGA
ACTCAGCGAACCGGCCGCGGCCGGCAAGGGCGGTTCGTCGACGATGCCGCACAAGCGCAATCCGGTCGGCTGCGCCGCCG
TGCTGACGGCCGCGGTGCGCGCACCGGGCCTCGTCGCGACCGTGTTCGCGGGGATGGTCCAGGAACACGAGCGCGCGCTC
GGCGGCTGGCAGGCCGAGTGGGACGCGCTGCCCGACCTCGCGCGCCTCGCCGGCGGCGCACTCGCGCAGATCGCGCAGAT
CGTTGCGGGCCTCGACGTGAACGCCGAACGCCTCGCGGCGAACCTCGACCTCACGCACGGCTTGATCCTCGGCGAAGCGG
TGATGCTCGCGCTCGGCGACCGGATCGGCCGTCTCGACGCGCATCACGTCGTCGAGCACGCGTCGAAGGAAGCCGTGCGC
ACCGGCGCGACGCTGTTCGACGTGCTCGCCGCCGACGCGACCGTGTCGGCCCACCTGTCGCGCGACGCGCTCGCACAGTT
GCTCGACCCCGCTCAATACGTCGGCGAGGCGCATGCCTACGTCGACGCCGTGCTCGCACTGCACGCGCGCACGTAA

Upstream 100 bases:

>100_bases
CGACGCGCAAGGCGGCCTGAACCGCAAGCGGCCGGCGCGCGCGTGCGCACGCCGGCGCGCTTCGGTGGACAATAGGCCAC
CCGTTTTCCCATCCGACGCC

Downstream 100 bases:

>100_bases
CCACCAGGAGAACACCGATGCCTTTCGCCACCGTCAACGGCGTAAAACTGCATTACCGGATCGATCGTGCCGCGCGCGAC
GACGCGCCGTGGCTCGTCTT

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLEDSARLTSLICGIEPLNRIWSPRATVQRMLDVEAALARALAAQQVIPAAAVAPIERACVADRLDADALARDAALGGNL
AIPLVKQLTAQVKADDAEAAKFVHWGATSQDIIDTATVLQLRDTLDVLEPMLDDACASLATLARTHRATPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRTRLAELRARALVLQFGGAAGTLASLRDRASGVSAALAKELELAAPAVPWHTQRDRIAEAA
AWFGMLIGTLGKIARDISLQMQTEVGELSEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVATVFAGMVQEHERAL
GGWQAEWDALPDLARLAGGALAQIAQIVAGLDVNAERLAANLDLTHGLILGEAVMLALGDRIGRLDAHHVVEHASKEAVR
TGATLFDVLAADATVSAHLSRDALAQLLDPAQYVGEAHAYVDAVLALHART

Sequences:

>Translated_451_residues
MLEDSARLTSLICGIEPLNRIWSPRATVQRMLDVEAALARALAAQQVIPAAAVAPIERACVADRLDADALARDAALGGNL
AIPLVKQLTAQVKADDAEAAKFVHWGATSQDIIDTATVLQLRDTLDVLEPMLDDACASLATLARTHRATPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRTRLAELRARALVLQFGGAAGTLASLRDRASGVSAALAKELELAAPAVPWHTQRDRIAEAA
AWFGMLIGTLGKIARDISLQMQTEVGELSEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVATVFAGMVQEHERAL
GGWQAEWDALPDLARLAGGALAQIAQIVAGLDVNAERLAANLDLTHGLILGEAVMLALGDRIGRLDAHHVVEHASKEAVR
TGATLFDVLAADATVSAHLSRDALAQLLDPAQYVGEAHAYVDAVLALHART
>Mature_451_residues
MLEDSARLTSLICGIEPLNRIWSPRATVQRMLDVEAALARALAAQQVIPAAAVAPIERACVADRLDADALARDAALGGNL
AIPLVKQLTAQVKADDAEAAKFVHWGATSQDIIDTATVLQLRDTLDVLEPMLDDACASLATLARTHRATPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRTRLAELRARALVLQFGGAAGTLASLRDRASGVSAALAKELELAAPAVPWHTQRDRIAEAA
AWFGMLIGTLGKIARDISLQMQTEVGELSEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVATVFAGMVQEHERAL
GGWQAEWDALPDLARLAGGALAQIAQIVAGLDVNAERLAANLDLTHGLILGEAVMLALGDRIGRLDAHHVVEHASKEAVR
TGATLFDVLAADATVSAHLSRDALAQLLDPAQYVGEAHAYVDAVLALHART

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=366, Percent_Identity=25.4098360655738, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=363, Percent_Identity=25.6198347107438, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32564234, Length=247, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=366, Percent_Identity=24.5901639344262, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647570, Length=205, Percent_Identity=33.1707317073171, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47546; Mature: 47546

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDSARLTSLICGIEPLNRIWSPRATVQRMLDVEAALARALAAQQVIPAAAVAPIERAC
CCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VADRLDADALARDAALGGNLAIPLVKQLTAQVKADDAEAAKFVHWGATSQDIIDTATVLQ
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LRDTLDVLEPMLDDACASLATLARTHRATPMIGRTWLQQALPITLGLKFAQWLDALLRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TRLAELRARALVLQFGGAAGTLASLRDRASGVSAALAKELELAAPAVPWHTQRDRIAEAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AWFGMLIGTLGKIARDISLQMQTEVGELSEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLVATVFAGMVQEHERALGGWQAEWDALPDLARLAGGALAQIAQIVAGLDVNAERLAA
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCHHHHHHHHHHHCCCCCHHHHHH
NLDLTHGLILGEAVMLALGDRIGRLDAHHVVEHASKEAVRTGATLFDVLAADATVSAHLS
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDALAQLLDPAQYVGEAHAYVDAVLALHART
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEDSARLTSLICGIEPLNRIWSPRATVQRMLDVEAALARALAAQQVIPAAAVAPIERAC
CCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VADRLDADALARDAALGGNLAIPLVKQLTAQVKADDAEAAKFVHWGATSQDIIDTATVLQ
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LRDTLDVLEPMLDDACASLATLARTHRATPMIGRTWLQQALPITLGLKFAQWLDALLRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TRLAELRARALVLQFGGAAGTLASLRDRASGVSAALAKELELAAPAVPWHTQRDRIAEAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AWFGMLIGTLGKIARDISLQMQTEVGELSEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLVATVFAGMVQEHERALGGWQAEWDALPDLARLAGGALAQIAQIVAGLDVNAERLAA
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHCHHHHHHHHHHHCCCCCHHHHHH
NLDLTHGLILGEAVMLALGDRIGRLDAHHVVEHASKEAVRTGATLFDVLAADATVSAHLS
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDALAQLLDPAQYVGEAHAYVDAVLALHART
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]